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OfflineModestMouserrrrr
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Registered: 05/24/03
Posts: 10
Last seen: 21 years, 26 days
Temp at which SPORES die (in syringe form) not mycelium
    #1574682 - 05/24/03 02:36 PM (21 years, 2 months ago)

Ahoy!

I am a new member to the group, yet a long time browser of the site. 
I recall reading somewhere, at some time, that spores can take higher temperatures than the 106 degree F temp max that mycelium has, (mycelium death above 106) and it will still be ok to use when some degree of higher temps is reached.

Some guy I know in Japan was inquiring to me about it, and so im just gonna relay his info here.  He apperently injected some TC strain into some straight Rye bags, (nothing but sterelized rye, four of them, at 2cc in each, 3 bags had two injection points, at 1cc each. One bag had 4 injection points at half a cc each), and for the first 3 to 4 days the temperature flucuated from being safe (below 88 F) to possibly as high as 102 to 104 F.  He apperently is praying to his god that they will be allright. 

The higher numbers stated are probly a little higher than it got, but thats what he told me he estimates it at.  There was no thermometer available for the initial few days.  Now he has a nice in door / out door digitial thermometer plus hydometer.  can have temp inside next too bags, and temp outside on desk, weeeee.

He told me that the temps are stable now, right around 88, mostly a bit below.  Apperently he wouldn't have been worried, but its been 5 days since injection, and not a bit of action yet.  Just very light condensation on the sides of the bags.  While it is still very early, he just wanted to make sure that the heat issue at the beggining wasnt going to cauise for alot of needless waiting.


One last question, lets say hypothetically the bags got to 108 F, on day two after injection, would there be no chance, or is there a good chance they are ok since it isn't mycelium yet, and just spores.

Thanks in advance, im not sure what all this means really, I just copied and pasted what he emailed to me.  I think it has to do with cooking tv dinners without a microwave.  :confused:

Thanks again guys!

-ModestMouser 

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OfflineI3lunts42o
Spore Collector
Registered: 07/29/02
Posts: 204
Loc: I don't even know where i...
Last seen: 19 years, 10 months
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: ModestMouserrrrr]
    #1574905 - 05/24/03 05:11 PM (21 years, 2 months ago)

i beleive i read on this site that spores die at 106 F. do a search on "thermal death"


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Note: This post is a work of fiction and for entertainment only.
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I smoke weed to ease my mind,
I eat every tab i find,
I drink shroom tea by the jugs,
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Offlinejehst
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Registered: 05/18/03
Posts: 114
Last seen: 18 years, 10 months
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: I3lunts42o]
    #1574975 - 05/24/03 05:57 PM (21 years, 2 months ago)

i remember 106 too


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All I have in this world are my balls and my word, and I don't break 'em for no one. You understand me?

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OfflineModestMouserrrrr
Stranger
Registered: 05/24/03
Posts: 10
Last seen: 21 years, 26 days
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: jehst]
    #1575040 - 05/24/03 06:36 PM (21 years, 2 months ago)

I did do some reseach. I found a *very* short quote from a ANNO on a post that said the following -

>I read that thermal death occurs at 104F.
ANNO-
"For mycelium, but not for spores.
I guess for spores it?s quite higher. "


This post was located at http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=691974&Forum=Forum2&Words=thermal%20death&Match=Entire%20Phrase&Searchpage=2&Limit=25&Old=allposts&Main=691924&Search=true#Post691974


Anyways, I'll keep lookin, any more info qould be helpfull,

-ModestMouser


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Offlinejakeholman
crotch rocketsrule!

Registered: 02/24/03
Posts: 503
Last seen: 17 years, 4 months
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: ModestMouserrrrr]
    #1575559 - 05/24/03 11:39 PM (21 years, 2 months ago)

if you see no contams or growth in a week than you might be able to get away with knocnking them up for round number two. and by the way, if you plan on using that digital hygro/thermo for your terrarium, be prepared for the temp unit to quit working-the foafs did after a month or two, but the hygro is awesome.


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The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.

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OfflineModestMouserrrrr
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Registered: 05/24/03
Posts: 10
Last seen: 21 years, 26 days
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: jakeholman]
    #1575853 - 05/25/03 02:52 AM (21 years, 2 months ago)

Go out as in read wrong temperatures, or what? Was it bolth the external and internal thermometers? Wonder why that would be... humidity mabey?


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Offlinejakeholman
crotch rocketsrule!

Registered: 02/24/03
Posts: 503
Last seen: 17 years, 4 months
Re: Temp at which SPORES die (in syringe form) not mycelium [Re: ModestMouserrrrr]
    #1576563 - 05/25/03 03:49 PM (21 years, 2 months ago)

the foafs humidity/temp gauge was in a terrarium (above 90% rh) and the temp unit in the gauge froze at where it was, came back once or twice then never fixed itself-i am assuming that the humidity did it, but what a dumbass way to build a 2in1 thing that doesnt work in humid areas.
Jake H


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The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.

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Offlineresin
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Registered: 06/12/02
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: jakeholman]
    #1576698 - 05/25/03 05:22 PM (21 years, 2 months ago)

Yours did that shit too? I thought I stepped on it or somthing. The fucks build it so it will break and you gotta buy a new one.

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OfflineChrisH
Dang ole' dang
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: resin]
    #1577127 - 05/25/03 09:24 PM (21 years, 2 months ago)

Get one of those stick on fish tank thermomaters.I have one on my incubator and another on my terranium.They work great!


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Are you sure you want to eat those?:mushroom2:

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OfflineChrisH
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: ChrisH]
    #1577132 - 05/25/03 09:26 PM (21 years, 2 months ago)

thermometers


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Are you sure you want to eat those?:mushroom2:

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OfflineCorrosiveLiquid
Amature Mycologist
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Registered: 02/05/10
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: ModestMouserrrrr]
    #11981374 - 02/07/10 12:00 PM (14 years, 5 months ago)

whats the lowest temps they can survive? syringe and prints...


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"In order to be a leader, you must FIRST be a follower!"

"Emancipate yourselves from mental slavery, None but ourselves can free our minds."
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InvisibleShea25
Just some guy
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Registered: 01/27/09
Posts: 7,772
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: CorrosiveLiquid]
    #11981394 - 02/07/10 12:04 PM (14 years, 5 months ago)

I have had syringes freeze on me and still work fine

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OfflineCorrosiveLiquid
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: Shea25]
    #11981406 - 02/07/10 12:06 PM (14 years, 5 months ago)

cool, just wondering because we just got over 2' of snow and its like 20F outside..


--------------------
"In order to be a leader, you must FIRST be a follower!"

"Emancipate yourselves from mental slavery, None but ourselves can free our minds."
"Don't gain the world and lose your soul, Wisdom is better than silver and gold"

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InvisibleJonEveryman88
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Registered: 01/18/10
Posts: 1,638
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: CorrosiveLiquid]
    #11981417 - 02/07/10 12:08 PM (14 years, 5 months ago)

I think noobie shroomers successfully froze his spores in the freezer and then used them. I can't remember his success though...

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InvisibleShea25
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: CorrosiveLiquid]
    #11981429 - 02/07/10 12:09 PM (14 years, 5 months ago)

Quote:

CorrosiveLiquid said:
cool, just wondering because we just got over 2' of snow and its like 20F outside..





They should survive 20F/-6C.

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OfflineRogerRabbitM
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Re: Temp at which SPORES die (in syringe form) not mycelium [Re: Shea25]
    #11981728 - 02/07/10 01:03 PM (14 years, 5 months ago)

Normal indoor room temperature is what you want to grow at.  There is no reason to exceed 26C at any time.

Spores die from being exposed to a temperature at or above 140F/60C for an hour.  Some die sooner than that, but the above nukes them all.
RR


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