Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineKissmegxxdnite
Breath in Electronic Fumes
 User Gallery
Registered: 12/15/11
Posts: 190
Loc: Oregon Flag
Last seen: 12 years, 15 days
Curious about GE with lc.
    #15745884 - 02/01/12 03:21 AM (12 years, 11 months ago)

I am doing the self healing injection port baby bottle lc tek. With this method there is no gas exchange. I had an idea and would enjoy some input. I was going to stuff an old syringe with polyfill pc for a bit 15-20 minutes with it wrapped in tin foil. Inoculate these and afterwords stick the syringe with polyfill in the injection port an let it sit til it's done growing pulling it out to swirl. Is this an alright idea or a horrible one.

Extras: Filter Print Post Top
Offlinekrum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 11 years, 5 months
Re: Curious about GE with lc. [Re: Kissmegxxdnite]
    #15745895 - 02/01/12 03:32 AM (12 years, 11 months ago)

thats what you wanna do.  If you have micropore that would work probs a little better than the polyfill, but you got the idea.  Here is the thing, when those baby bottles cool down after pcing, there will be a vacuum in there, thats when you wanna hit it with the polyfil syringe to alleviate the pressure, NOT AFTER.  LC's don't really need much GE.  I know some guys keep it in, i find it unnecessary and its also another possible contamination vector

Extras: Filter Print Post Top
OfflineKissmegxxdnite
Breath in Electronic Fumes
 User Gallery
Registered: 12/15/11
Posts: 190
Loc: Oregon Flag
Last seen: 12 years, 15 days
Re: Curious about GE with lc. [Re: krum]
    #15745899 - 02/01/12 03:36 AM (12 years, 11 months ago)

I'll just leave it out I have had success without GE so I'll stick with what works. Thanks for the input.

Extras: Filter Print Post Top
Offlinekrum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 11 years, 5 months
Re: Curious about GE with lc. [Re: Kissmegxxdnite]
    #15745903 - 02/01/12 03:38 AM (12 years, 11 months ago)

so what do you do about the vacuum

Extras: Filter Print Post Top
OfflineKissmegxxdnite
Breath in Electronic Fumes
 User Gallery
Registered: 12/15/11
Posts: 190
Loc: Oregon Flag
Last seen: 12 years, 15 days
Re: Curious about GE with lc. [Re: krum]
    #15745909 - 02/01/12 03:42 AM (12 years, 11 months ago)

There's two methods the polyfill Syringe method and pc'ing with loose lids.

I'll alleviated the vacuum and then noc up.

Edited by Kissmegxxdnite (02/01/12 03:43 AM)

Extras: Filter Print Post Top
Offlinekrum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 11 years, 5 months
Re: Curious about GE with lc. [Re: krum]
    #15745912 - 02/01/12 03:43 AM (12 years, 11 months ago)

cuz if you're like "what vacuum" and the negative pressure isn't drawing in the syringe you're inoculating with, you've got all the GE you need for an LC.  But, that also means you've got a hole somewhere raw air can re-enter...thats another contamination vector, unless its taped around the threads.

Extras: Filter Print Post Top
Offlinekrum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 11 years, 5 months
Re: Curious about GE with lc. [Re: krum]
    #15745925 - 02/01/12 03:48 AM (12 years, 11 months ago)

yeah, thats fine, just remember PCing with loose lids should probably be re tightened once you transfer it from the PC when its cool, preferably inside a glove box or flow hood. 

My trick now for doing open air inoculations, is having a really fucking hot alcohol burning stove where I'm working.  Not sure why it works or the physics behind it, but proofs in the pudding.

Extras: Filter Print Post Top
OfflineKissmegxxdnite
Breath in Electronic Fumes
 User Gallery
Registered: 12/15/11
Posts: 190
Loc: Oregon Flag
Last seen: 12 years, 15 days
Re: Curious about GE with lc. [Re: krum]
    #15745937 - 02/01/12 03:55 AM (12 years, 11 months ago)

I have 3 tight and 3 loose. I was going to tighten the loose ones in the pc right after opening. Unless a transfer to my glove box would be better.

Extras: Filter Print Post Top
Offlinekrum
la muerte peluda


Registered: 07/30/10
Posts: 557
Last seen: 11 years, 5 months
Re: Curious about GE with lc. [Re: Kissmegxxdnite]
    #15752694 - 02/02/12 05:50 PM (12 years, 11 months ago)

nope, that'll do it.  Gloved/sanitized hands, your myc will thank you

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


Similar ThreadsPosterViewsRepliesLast post
* LC and polyfill davesj1 594 1 06/09/06 05:08 PM
by Citric
* Sporeprint to Agar to LC to Innoc wiggles 5,189 6 07/01/11 07:53 PM
by ely2121
* How's my LC lid? (pic) Alprazolom 1,053 8 10/27/06 04:14 PM
by nuk1m
* stalled LC questions eclipse 1,382 6 08/17/06 06:45 PM
by StinkFist13
* LC lids that don't leak
( 1 2 all )
agar 9,422 23 02/20/06 09:31 AM
by musher_420
* LC lids faceyneck 4,798 11 02/11/07 09:09 AM
by agar
* Vacuum in LC jar modrans 1,407 7 08/03/06 03:39 PM
by kristen
* Vacuum in LC Jar etam 2,897 11 09/27/11 11:41 AM
by Trypset

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
381 topic views. 11 members, 70 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 12 queries.