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OfflineJw6457
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Day 10 of trays little colonization please help
    #15745046 - 01/31/12 10:27 PM (12 years, 11 months ago)

Hey guys, I have 4 small trays each with a Brf cake grated with a couple bulk sub mixes. The problem is, I checked them 5 days in to see if they were even colonizing cause it was first time and though they'd contam for sure. I have clear trays so I just looked on the sides and didn't take the foil lid off. Bits it's been over 5 days since I last checked them and there hasn't been any myc progression at all and fearing they are done. They still look white but no growth in 5 days. I thought its only suppose to take 10-11 days to re-colonize the bulk sub. What should I do? Toss them, as far as I can tell the myc still looks white and no contams from the side but is it to late for them.


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WHAT UP GUYS< JUST HERE TRYING TO BECOME A SCIENTIST AND GROW SOME MOOSHIES

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OfflineSwwert
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Re: Day 10 of trays little colonization please help [Re: Jw6457]
    #15745244 - 01/31/12 11:15 PM (12 years, 11 months ago)

Just wait it out 5-10 more days. How was the moisture in your substrate? What is your temperature?
Need more info on your substrate.


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Whole Brown Rice Cakes TEK
   
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InvisibleJavadog
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Re: Day 10 of trays little colonization please help [Re: Swwert]
    #15745295 - 01/31/12 11:34 PM (12 years, 11 months ago)

When you spawn a cake (or grains for that matter) to a bulk sub, some
bits of spawn will inevitably end up nearer the surface than others.
When this happens you will see white spots on the surface of the sub
that will fuzz up but not immediately spread.  This is the spawn
recovering.

I wonder if this is what you are seeing.

In any case, do not dump the tray until you see a problem.

Let us know what you see at 15 days.

Good luck!

JD


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OfflineJw6457
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Re: Day 10 of trays little colonization please help [Re: Javadog]
    #15748761 - 02/01/12 07:29 PM (12 years, 11 months ago)

well, i just looked through the sides of the trays and did not lift the tinfoil lid off the trays yet so i dont know what the top looks like. from the sides its white blotchy but defintaely does not look anywhere near colonized. When i spawned to the bulk sub, i could take a handful and squeeze as hard as i could and only a few drops fell out. I can tell though the the substrate looks much wetter then when i spawn by having a lid over the tray. Could that be the problem?


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WHAT UP GUYS< JUST HERE TRYING TO BECOME A SCIENTIST AND GROW SOME MOOSHIES

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InvisibleJavadog
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Re: Day 10 of trays little colonization please help [Re: Jw6457]
    #15749094 - 02/01/12 08:31 PM (12 years, 11 months ago)

Yes, a wet sub can slow colonization.


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InvisibleWilly Wonka
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Re: Day 10 of trays little colonization please help [Re: Jw6457]
    #15749256 - 02/01/12 09:03 PM (12 years, 11 months ago)

You said you squeezed your bulk substrate as hard as you could and only a little bit of water dripped out? If I'm reading your post right and that is what you mean then this could be your problem.  If this is the case then your substrate is to dry.  When you are adding water to your bulk substrate you are trying to bring the substrate moisture to "Field Capacity."  Feild capacity is when you've added enough water to the substrate that when you pick a handful up and just hold it without squeezing no water drips out.  But then when you squeeze lightly only a few drops drip out.  And then when you squeeze the bulk substrate hard a small rivulet pours out the bottom of your hand for a few seconds then it dries up, a few more drips come out and then the water flow stops on it's own. 

If you squeezed your substrate real hard and nothing came out but a few drops then there is where your problem lie


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OfflineJw6457
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Re: Day 10 of trays little colonization please help [Re: Willy Wonka]
    #15749270 - 02/01/12 09:07 PM (12 years, 11 months ago)

alright the only thing is, is that the space between the tinfoil lid and the substrate looks dam wet compared to what it was, thats the only thing im worrying about


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WHAT UP GUYS< JUST HERE TRYING TO BECOME A SCIENTIST AND GROW SOME MOOSHIES

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OfflineWing
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Re: Day 10 of trays little colonization please help [Re: Willy Wonka]
    #15749714 - 02/01/12 10:37 PM (12 years, 11 months ago)

Quote:

Willy Wonka said:
If you squeezed your substrate real hard and nothing came out but a few drops then there is where your problem lie




Squeeze as hard as you can and only a few drops should come out. You want your substrate damp, not wet. There should be no rivulet. That's too wet.

If your substrate is too wet it will slow down colonization and encourage bacterial breeding.

A good rule of thumb is to err on the side of too dry. You can always sterilize water and add it. It's damn near impossible to take water out if it's too wet.


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OfflineKizzle
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Re: Day 10 of trays little colonization please help [Re: Wing]
    #15749862 - 02/01/12 11:15 PM (12 years, 11 months ago)

Yeah they will colonize fine a little dry but a little wet will slow things way down. Far too wet can prevent colonization completely if not the whole tub at least parts of it.

You should also consider that the condensation may make it appear more wet than it really is. 5 days is a bit early to be worrying though I would expect to see some significant growth. 10 days though... I don't know.


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InvisibleWilly Wonka
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Re: Day 10 of trays little colonization please help [Re: Wing]
    #15754016 - 02/02/12 10:41 PM (12 years, 11 months ago)

Quote:

winged_1 said:
Squeeze as hard as you can and only a few drops should come out. You want your substrate damp, not wet. There should be no rivulet. That's too wet.




That information is wrong.  Sorry winged_1 but it is just plain wrong.  I am going to quote Roger Rabbit on this one.  In his video, "Let's Grow Mushrooms"  RR tells us that your bulk substrate needs to be at "Field Capacity."  Then he goes on to define field capacity as,

1) Pick up a small handful. No water should drip.
2) Squeeze gently.  One or two drops will fall. 
3) Squeeze hard. A small stream will flow then stop after a second or two.


You can check for yourself here.  50 seconds into the preview RR says it himself.  I would recommend you check out the video winged_1.


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AMU


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InvisibleJavadog
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Re: Day 10 of trays little colonization please help [Re: Willy Wonka]
    #15754080 - 02/02/12 10:58 PM (12 years, 11 months ago)

The weird thing is that I have tended toward the few drips that
winged refers to...suffice it to say that it is far better to have
a sub on the dry side than the wet.

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

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OfflineWing
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Re: Day 10 of trays little colonization please help [Re: Javadog]
    #15755524 - 02/03/12 11:20 AM (12 years, 11 months ago)

:whathesaid:

I know the video. I've seen a lot bacterial contamination from overly wet substrates. You will have more than enough moisture from making it drier than that video says.

Bacterial contamination is very common. An experienced eye will notice it in a lot of grows on these forums. It mostly goes unnoticed and the substrate usually fruits but it's there and it can hinder a grow.

By all means use as much water as you feel you can, I'm not saying its a bad thing. But to err on the safer side/drier side you'll still get great results, if not better :shrug:


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InvisibleWilly Wonka
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Re: Day 10 of trays little colonization please help [Re: Wing]
    #15757367 - 02/03/12 07:05 PM (12 years, 11 months ago)

The OP didn't state if  the bulk substrate is coir and verm or manure.  But if the OP is using coir and the coir is properly sterilized or if the OP is using manure and the manure is properly pasteurized then there shouldn't be any bacterial contamination.  That video is tried tested and true.  But as you said:shrug:


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OfflineDiesel_Dawg
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Re: Day 10 of trays little colonization please help [Re: Willy Wonka]
    #15758771 - 02/04/12 01:46 AM (12 years, 11 months ago)

Coir is supposed to be pasteurized too, not sterilized, but I agree with your water content remarks. If you squeeze as hard as you can and only get a couple drops, thats too dry. I squeeze mine firmly and see 8-10 droplets maybe. and if I squeeze as hard as I can quite a bit more will come out.. And that gives me 7-10 day colonization on my mono tubs in most cases.

You guys are correct in stating that it will still colonize, but if you want it to fruit and expect your substrate to support a solid flush it needs more moisture.

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OfflineWing
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Re: Day 10 of trays little colonization please help [Re: Diesel_Dawg]
    #15765250 - 02/05/12 05:28 PM (12 years, 11 months ago)

Different strokes for different folks. I know region and supplies can make a big difference in mycology. Forbme, my way works but it might not work as well when someone else does it.

I don't think the OP has issues with it being too dry since in his second post he mentions it being wet in there.


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