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OfflineFriendofyours
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a little help plz
    #15738635 - 01/30/12 03:20 PM (12 years, 11 months ago)

OK so 2 weeks ago I inoculated some wbs jars with a multi spore syringe from Freespores.com.

The mycelium that grew appeared at the innoculation points on each jar and appeared to be off white.

At the 2 week mark the jars were maybe 40% colonized so I went ahead and shook them. Its been two days since then and they appear to be recovering slowly.

I froze the grain for a few weeks before PCing them. After PC I did notice condensation inside the jars but the grain isn't soaking wet or anything.

My jars are colonizing on the top shelf of my closet. The temp inside my house is 68 degrees.

Also when I got my syringe there was mycelium already germinated inside it.

Sorry this is kinda long but I want to get all the info out there so that maybe someone can tell me why they are growing so slow.

I don't believe its a contamination Because there would be mycelium piss right?

Plz help ill get some pic up if I can but like I say they've been shook already.

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OfflineSteezMe
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Re: a little help plz [Re: Friendofyours]
    #15738700 - 01/30/12 03:35 PM (12 years, 11 months ago)

When you shake the jars your myc gets damaged and you have to give it time to recover.. Usually takes a few days but then it takes off and takes off quick.. Give it time.. Patience is king in this trade..


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Offlinecrimsondrac
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Re: a little help plz [Re: Friendofyours]
    #15738711 - 01/30/12 03:36 PM (12 years, 11 months ago)

Are you sure you ordered a Spore syringe?  Some vendors sell Liquid Culture (LC) syringes that do have mycelium in them already. You can actually grow the mycelium in a sugar/water solution and inoculate your substrate using that. This method helps speed up colonization.

No one on this site will be able to help you with contamination issues without any pictures. Myc piss does not need contamination to occur. Not sure why you froze your grain and I am not sure if that will hurt anything, but as long as you have myc growth and no discoloration, you are generally ok.

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Invisibleslapphappypill
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Re: a little help plz [Re: crimsondrac]
    #15738738 - 01/30/12 03:41 PM (12 years, 11 months ago)

so far you sound ok, give those jars another 2-7 days and they could be close to 100%.

as stated above, Patience is key, just watch the jars and see what happens (there's nothing you can do any further now anyway, so play the waiting game and do some more jars in the meantime :hamsterdance:)


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We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineFriendofyours
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Re: a little help plz [Re: crimsondrac]
    #15738771 - 01/30/12 03:46 PM (12 years, 11 months ago)

I froze my grain Because I couldnt pressure cook it at the time.
Yes I ordered a spore syringe.
I know what a liquid culture is I curriently have 3 of them.
My mycelium is indeed discolored.
And there is no sign of any other mold growing and no mycelium pee.

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Offlinecrimsondrac
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Re: a little help plz [Re: Friendofyours]
    #15738797 - 01/30/12 03:53 PM (12 years, 11 months ago)

I would check with freespores then to see if they even sent you the right stuff. It is possible they sent you a contaminated syringe. As hard as every one tries to stay sterile, contamination does occur from time to time. Again, pics or no one will be able to help much.

Did you follow tek for Rye grain?  Soak, rinse, simmer, Pressure cook, wait a few days, then innoc?  Maybe you did not get all the endospores.

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OfflineFriendofyours
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Re: a little help plz [Re: crimsondrac]
    #15738904 - 01/30/12 04:18 PM (12 years, 11 months ago)

I prepared my wbs by submerging the wbs in water, removed the floaters, then soaked for 36 hours changing the water every 12 hours along with rinsing each time.

After that I froze the wbs for a lil over 2 weeks. When I was ready I thawed, rinsed, and loaded up jars. Put foil and rubber bands on them and PC for one hour. Then I let them sit over night before innoculating them.

I made self healing injection ports for all of my jars and polyfil filters for gas exchange.

When I inoculated them I shut off my AC, sealed off the door, sprayed lysaw like a mad man LOL, cleaned my work surface with alcohol,  then I sprayed again then removed foil and began innoculating jars ,wiping and wrapping the needle in a alcohol soaked syringe each time I used it.

I did not flame steralize the syringe Because it came in what I believed to be a presteralized wrapper. It was new.

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Invisibleshroomerite
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Re: a little help plz [Re: Friendofyours]
    #15738944 - 01/30/12 04:27 PM (12 years, 11 months ago)

You should still flame sterilize in between each jar. It will keep you safe in the long run. Freezing it didn't hurt it unless it busted the grains. If not you should be ok. Two days isn't that long for a recovery. After day three things should be speeding along.


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  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR


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OfflineFriendofyours
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Re: a little help plz [Re: shroomerite]
    #15738980 - 01/30/12 04:35 PM (12 years, 11 months ago)

You may have just answered my question. I did notice quite a few Poped grains. Not like 50% or anything but deffinetly arround 10 to 15 % 

Can you tell me why poped grains are no good I would appreciate it a lot. :smile:

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Invisibleslapphappypill
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Re: a little help plz [Re: Friendofyours]
    #15739137 - 01/30/12 05:12 PM (12 years, 11 months ago)

Quote:

shroomerite said:
You should still flame sterilize in between each jar. It will keep you safe in the long run. Freezing it didn't hurt it unless it busted the grains. If not you should be ok. Two days isn't that long for a recovery. After day three things should be speeding along.




Quote:

Friendofyours said:
You may have just answered my question. I did notice quite a few Poped grains. Not like 50% or anything but deffinetly arround 10 to 15 % 

Can you tell me why poped grains are no good I would appreciate it a lot. :smile:





Just to throw my 2 cents in.... I've used WBS spawn that consisted entirely of busted and burnt kernels... All 14+ jars that I knocked up grew perfect myc that was spawned successfully and fruited perfectly

I've done the same thing with burnt/busted sunflower seeds... (I also never remove floaters)

As long as you PC for 90 minutes, (120 if you have larger bags etc) you're fine with not worrying about contams.

IF you indeed do have contams, it's most likely due to your syringe sterility, or only PCing your grain for 90 minutes.


HOWEVER, you seem to have skipped over my original post completely.. Give your jars more time to see if there are indeed any contams.

But hey, what do I know? :shrug: I've only spawned upwards of 1000+ qt jars in the last year to hundreds of bags/tubs etc without a single contamination in my grains...


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineFriendofyours
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Re: a little help plz [Re: slapphappypill]
    #15739249 - 01/30/12 05:35 PM (12 years, 11 months ago)

My appologies I did not overlook your post. I agree patients is like 75% of this hobby. Its just I love to learn and find answers to interesting questions. And I really want to know what causes mycelium
to be discolored (greyish blue)

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OfflineSteezMe
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Re: a little help plz [Re: Friendofyours]
    #15740015 - 01/30/12 08:00 PM (12 years, 11 months ago)

Bruising is what makes it that grayish blue color..


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OfflineFriendofyours
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Re: a little help plz [Re: SteezMe]
    #15744247 - 01/31/12 07:20 PM (12 years, 11 months ago)

Well last night I descided to shoot up my jars with live tissue from one of my LCs of pf classic.

Will the mycelia even take hold? The other strain seems to have at least a speck of growth on each curnal of grain but so far isn't forming huge masses ( just some little ones )

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OfflineSteezMe
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Re: a little help plz [Re: Friendofyours]
    #15744449 - 01/31/12 08:04 PM (12 years, 11 months ago)

Did you shoot up already colonizing jars with an lc...?


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OfflineFriendofyours
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Re: a little help plz [Re: SteezMe]
    #15744457 - 01/31/12 08:07 PM (12 years, 11 months ago)

Yulp

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InvisibleWilly Wonka
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Re: a little help plz [Re: Friendofyours]
    #15744485 - 01/31/12 08:13 PM (12 years, 11 months ago)

To answer your original question, (cubensis right?) I would guess temp.  I've always seen a slower growth when the temperatures drop down below 70f.  I've yet to see the rate of growth of jars in a good solid high 70s low 80s so I can't comment on that, but I am thinking about buying an aquarium heater so I can set my jars in a tote filled with water and place the heater in the tote to help maintain a good warm temp during the colder months.  You might want to try that?


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AMU


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OfflineSteezMe
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Re: a little help plz [Re: Willy Wonka]
    #15744673 - 01/31/12 09:00 PM (12 years, 11 months ago)

Why in the world would you shoot lc into already colonizing jars....?


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OfflineFriendofyours
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Re: a little help plz [Re: SteezMe]
    #15745582 - 02/01/12 01:00 AM (12 years, 11 months ago)

To see what would happen.

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OfflineSteezMe
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Re: a little help plz [Re: Friendofyours]
    #15745860 - 02/01/12 03:03 AM (12 years, 11 months ago)

Well.. I would suggest against it next time.. It can mess with your moisture content and raise your chances of contams ten fold.. There is a reason people don't inncoluate throughout the colonization period... Don't you think if it were smart to wait until 50% colonization and then inoculate it again someone would have told you about it by now..
I'm sorry if I come off as an asshole here, but the best chances of success you will have is finding and simple and proven tek and following it to a T... Be patient brother and let your myc do it's work :laugh:
Good luck with your future grows and enjoy your shroomies :P


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OfflineFriendofyours
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Re: a little help plz [Re: SteezMe]
    #15745879 - 02/01/12 03:18 AM (12 years, 11 months ago)

Yea I did notice an increase in the amount of condensation inside the jars but with my highest possable room temperature being 72 degrees I wasn't to conserned.

But wait I know contaminations like cobweb mold favior high humidity but can it colonize in a place with a low temp but  high humidity?

And thanks for all the help by the way I appreciate it a lot :smile:

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