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Offlinerony-pony
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Registered: 03/17/11
Posts: 14
Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: rony-pony]
    #15732797 - 01/29/12 09:02 AM (12 years, 11 months ago)

I went to three different pharmacies, none had any, one just didn't have any in stock which is good news for future but for now it leaves me in trouble - I picked up cream peroxide 6%, a hair product but I'm not too sure if this will have the desired effect. Obviously if there's H2O2 in it that's great but it will also have other things in it. What do you think? give it a bash?
I'm going away for a few days first thing in the morning so would rather do something now before it's too late

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Invisibleoysterizer
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Registered: 12/20/11
Posts: 183
Re: liquid culture inoculation [Re: rony-pony]
    #15732865 - 01/29/12 09:30 AM (12 years, 11 months ago)

Hmm, as far as i know benzoyl peroxide is used for hair bleaching/dye.
Benzoyl peroxide is usually prepared by treating hydrogen peroxide with benzoyl chloride, and i have no idea how that will affect the sub in the long run. It leaves behind benzoic acid as it breaks down,which its salts are a food preservative with antifungal properties. Although it inhibits mold yeast and bacteria i havent found anything that says it inhibits mushroom myc.
It could be an interesting experiment, but risky. If your situation is bad it may be worth a try diluted to 2% or 3%. Dilute some with  water and try on a small test area.

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Offlinerony-pony
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Registered: 03/17/11
Posts: 14
Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: rony-pony]
    #15732928 - 01/29/12 09:53 AM (12 years, 11 months ago)

I diluted the solution - added the same volume again in water - and gave a light spray to the vertical edges of the basket, and a couple of drops on the top. Situation isn't bad but if untreated it would go bad, just a couple of little wisps had appeared. Prevention is better than cure and all that... hopefully it will work out fine, either way, an experiment and chance to learn!

When I mentioned the hair dryer earlier, my thinking was that if the edges were dry then they'd be a lot less likely to host contams, like a kind of dry buffer area an inch thick all round, leaving the core to keep growing, it could also be worth a go next time. Or move on to the logs though from if they are less prone to contams.

With regards to LCs, can you effectively go on infinitely once up and running. ie LC from colonised grain(1), innoc more grain(2), LC from fruit of first grain, innoc more grain(3), LC from fruit of second set of grain, innoc.... and so on. What are common pitfalls?

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Invisibleoysterizer
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Registered: 12/20/11
Posts: 183
Re: liquid culture inoculation [Re: rony-pony]
    #15733146 - 01/29/12 11:19 AM (12 years, 11 months ago)

For the most part fresh air and control of humidity is the best way to prevent the cobweb from ever happening.
If you create dry areas with hair dryer your myc wont colonize well, and  potentially harmful mold can grow even if moisture content is low. Best to treat or cut away infected areas.
How are you providing fresh air exchange?

As cultures grow they essentially age. The more times a culture has been grown out and cloned the older and weaker it will become. You can do this for a while but eventually it will be to weak to colonize or fruit well. If you search senescence you should find out more. The closer the culture is to the original spores the younger it is. Agar in slants or petri dishes are good for making a master culture from a good clone, then using those to grow out new lc. You can use lc as a master culture and squirt a little into a fresh jar to become the next master, but eventually it will decline.It is advisable to learn to take spore prints of whatever your growing,so you can start over if cultures are lost to senescence or contam. One print should have enough spore to go the rest of your life.
  Contams are near impossible to see in liquid unless it goes completely cloudy. You always want to inoculate some test spawn first just to verify that there arent any unwelcome guests so you dont waste time and material growing a dozen jars of mold. I prefer agar to lc because contams are visible and you can rescue you cultures by transferring to a clean dish. One dish can make more spawn(or lc) than you know what to do with. Its pretty easy to do even with just a glovebox.

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Offlinerony-pony
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Registered: 03/17/11
Posts: 14
Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: rony-pony]
    #15733263 - 01/29/12 11:49 AM (12 years, 11 months ago)

I've been fanning the fruiting chamber 4-5 times per day, or basically every time I walk past it. I have been doing spore prints already and have had good success, the last one I done was huge and got me 30 syringes, each with visible spores, however each set of jars from these became contaminated, so I've been trying to learn about varying methods to see which works best for me. That's not to say I'll not do spore prints because of one failed batch, just trying to learn more and see which ways minimise the chance of contams. That way I am not relying soley on one method and can have a better all round understanding.
What is the best way to store a print?

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InvisiblePinsWellWithOthers
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Registered: 10/15/10
Posts: 1,834
Re: liquid culture inoculation [Re: rony-pony]
    #15733687 - 01/29/12 01:29 PM (12 years, 11 months ago)

.

Edited by PinsWellWithOthers (03/06/12 05:32 PM)

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Offlinerony-pony
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Registered: 03/17/11
Posts: 14
Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: PinsWellWithOthers]
    #15755098 - 02/03/12 09:05 AM (12 years, 11 months ago)

Cheers for the print tip.

Unfortunatley a bit of green mould took hold along with cobweb, it appeared in the last couple of days. The straw log was done three weeks ago today, so I guess there wasn't enough grain to completely colonise it quick enough.

Oh well, onwards and upwards, we learn from our mistakes

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