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OfflineWannakungfu
Khepri
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Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Introductions and spawn bags.
    #15724557 - 01/27/12 10:55 AM (12 years, 11 months ago)

Hello!
I'm Kungfu
or just fu.
I've been a lurker for a looooooong time.

My wife is tired of me just talking about mushrooms and has bought me an oyster mushroom growboxthingbagstuffawesomeness.
I have been brewing beer for years now, and always just composted, yarded or otherwise discarded my semi-spent grains(i can only achieve 60-70% starch conversion).  With this oyster colony I intend to re-purpose this grain as fungifood.  But being my first grow op, with sub optimal equipment and sub optimal skills I thought I'd better warn the community that an amateur is about to ruin a completely healthy spawn bag.

My technique is roughly outline as;
Drink Beer
Brew Beer
After sparging is complete;
place contents of grain bag(17lbs dry grain ~23-25 wet) into multiple ziploks.
drink beer
Using a method similar to that nicely informative MADE IN INDIAAAA video.  layer of grain, thinner layer of broken up spawn, layer of grain, layer of thinner broken up spawn, ?????, cake, layer of spawn, poke poke poke poke poke, and store.

As I suspect this house has never been used for such a purpose I'm not too concerned about cross contam of other fungi, I am worried about bacteria.  The grain is coming out of the mashing process at about 165F after about ~2.5 hours and is going into a still air chamber while i finish yeast pitch and bungin'.  Should be out of liquid and in chamber for about 30 minutes tops.  Whereupon I have to remove it to pour into the ziploks, exposing it to my dingy house's atmo.

Here's my request/ion I need first timer advice.  I don't expect amazing yeilds, perfect medical grade sterile conditions.  but should i attack my house with lysol before I do this? coat myself in bleach?  Sacrifice my Veinerdog to the old gods?  Shave my head? Add all my supplemental gypsum to the batch pee on it and do the hokey-pokey? Or is my tech just doomed from the start?  Please disregard that last bit.  I have read and watched alot about pftek, this process is 'similar' but i can't reproduce the handy 'glovebox' just yet am I doomed???

Love,
Kungfu

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Invisibleslapphappypill
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Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15724651 - 01/27/12 11:22 AM (12 years, 11 months ago)

Get a cheap pressure cooker from walmart, then load your spent grain into qt jars and PC for 90-120 minutes.

Inject your oyster culture

wait for 100%

Spawn to straw baskets (or other bulk sub/container)

You'll be fine without a GB if you keep area clean, wash with alcohol and spray air with lysol


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: slapphappypill]
    #15724735 - 01/27/12 11:50 AM (12 years, 11 months ago)

Thankyou for the advice, tho I have a spawn bag, no syringe.  And the bag arrived with a small hole in the very top of the bag, and an aborted fruit dehydrated in its open space inside the bag inside the box.  Having been expose to air without the benefit of the filter thats on the bag should i not us it, there looks to be an amount of metabolites and maybe a black mold.... wait I HAS CAMERA
AND NOW
to learn how to attach a picture....test test?


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Invisibleslapphappypill
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Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15724991 - 01/27/12 12:57 PM (12 years, 11 months ago)

that doesn't look good.... I'd send those pics to the company you bought that from and ask for a refund... or a new bag...


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: slapphappypill]
    #15725063 - 01/27/12 01:21 PM (12 years, 11 months ago)

Well thats just poopy then.  I was really stoked about this, I called the producers of the bag, described my issue, they told me it was perfectly healthy and to dunk it for 24 hours in cold water then let it fruit... when i told them i intended to break it up and use it to spawn on my beer grain the lady says " well we can't guarantee anything but we'd sure like to try your mushroom beer! " *facepalm.
I super appreciate your help.  any third opinions?

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Invisibleslapphappypill
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Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15725081 - 01/27/12 01:27 PM (12 years, 11 months ago)

Did you say you have a bulk substrate to spawn to?


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: slapphappypill]
    #15725229 - 01/27/12 01:56 PM (12 years, 11 months ago)

Kind of, I brew beer from all grain recipe's been brewing for a few years, always just toss the semispent grain like i said

I was going to inoculate the ziplocks full of my used grain with chunks of the oyster spawn bag and let them recolonize

EDIT:
Also I've contacted the company twice, once they told me it was perfectly fine, tho my first impression was one of infection.

I have since sent them a letter complaining about the 1/2inch opening at the top of the bag and the black growth on top of the brick but no response.

Edited by Wannakungfu (01/27/12 01:58 PM)

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Invisibleslapphappypill
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Registered: 11/07/10
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Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15725568 - 01/27/12 03:41 PM (12 years, 11 months ago)

Ok well I think i've overlooked the fact that this is a brick ready to fruit... not for spawning to more spawn...
Do this:

Fruit the brick as is (dunk etc as per instructions)

as you get oysters growing, pressure cook spent grains in a qt jar (or multiple jars).

In clean area with mask/gloves/oust, take a clean knife (I use papertowel soaked in alcohol 91% to wrap a knife/scalpel) and cut the oyster stem in half, then take a piece out (size of 1/2 a small fingernail) and drop into jar of grain.

You have to do it carefully to not get contams, but you'll be cloning your oyster and will have your own spawn.

After that jar colonizes, you'll be able to spawn to straw and really get your myco dreams to come true :bigjoint:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineDrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 5 years, 2 months
Re: Introductions and spawn bags. [Re: slapphappypill]
    #15725819 - 01/27/12 04:35 PM (12 years, 11 months ago)

That bag looks good to me. That company uses coffee grinds as the "bulk spawn" so that could explain the weirdness.

Give it a go or break it up into a bigger mono tub it'll do well.

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: DrCrumbs]
    #15725905 - 01/27/12 04:54 PM (12 years, 11 months ago)

I ABSOLUTELY <3 YOU GUYS.

I had arrived to the fact that the orangish liquid is likely due to the coffee grounds, and the black top also to the coffee grounds.

And its really that easy to clone mushrooms from pileus flesh??

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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 5 years, 2 months
Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15725935 - 01/27/12 05:02 PM (12 years, 11 months ago)

Yeah, I dunno if its really a clone unless its taken from the fruiting part and then you probably have a few strains anyway. But yeah the myc has a strong will to live and consume.

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: DrCrumbs]
    #15728647 - 01/28/12 09:41 AM (12 years, 11 months ago)

How much more successful is pileus/stape/mycelium cloning?  Can i just take some of the oysters cottony matt and just clone that or am i going to have more success using the fruit flesh(stape/pileus?)

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InvisibleJavadog
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Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15728770 - 01/28/12 10:27 AM (12 years, 11 months ago)

There is stuff to study on this.

There are recommended areas of the fruit.

Certain areas are more likely to grow aggressively.

Inside the stipe, near the base, is often recommended.

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineWannakungfu
Khepri
Male

Registered: 01/17/12
Posts: 12
Loc: American SW
Last seen: 12 years, 9 months
Re: Introductions and spawn bags. [Re: Wannakungfu]
    #15745323 - 01/31/12 11:43 PM (12 years, 11 months ago)

I went ahead with the spawn bag and already I can see the beginnings of the fruit, this bag was really ready to go.

WAAAAhhhh... here I go trying to post pictures for over an hour before I find the post about the not loading pictures bit.

Either way insert image of tiny fruits above that last line, and another pic of my brick floating in a bowl of water, turning it brown, under this bit.

Question that was raised was, this brick was exposed to the water for ~24 hours would the water left in the bowl be able to colonize?  Is there any  floating mycelium in any viable quantity in this water??

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates


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