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Offlinesleepyrz
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Registered: 12/19/09
Posts: 35
Last seen: 3 years, 7 months
yeast.....whats the point ? liquid culture
    #11733532 - 12/28/09 06:53 PM (14 years, 2 months ago)

title says it all

ive been reading for a few months now and i have yet to find a good writeup on yeast

what its used for,

why its beneficial,

in what situations do you use it

what kind is the kind i want,

should it be dead or alive?

is it for agar? can it be used in liquid culture?

if there is anyone who knows a bit about yeast and all the who what where when why and hows of it

please let a noob know

and i searched so please dont tell me to search

maybe im just a retarded searcher

so help me out if ya can


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InvisibleDoc_T
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Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11733579 - 12/28/09 07:01 PM (14 years, 2 months ago)

It's nutritious. I use it in LC all the time.
For our purposes, it's all the same- bread yeast, brewer's yeast, nutritional yeast.

Edit - Here is my LC recipe.


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You make it all possible. Doesn't it feel good?

Edited by Doc_T (12/28/09 07:08 PM)

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Invisiblefeelfunny
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Re: yeast.....whats the point ? liquid culture [Re: Doc_T]
    #11733607 - 12/28/09 07:06 PM (14 years, 2 months ago)

and knowing is half the battle


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
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OfflinerockNrave
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Registered: 08/11/09
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Re: yeast.....whats the point ? liquid culture [Re: feelfunny]
    #11733672 - 12/28/09 07:20 PM (14 years, 2 months ago)

Quote:

feelfunny said:
and knowing is half the battle




G I

JOOOOOEEEEE


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Invisiblefeelfunny
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Re: yeast.....whats the point ? liquid culture [Re: rockNrave]
    #11733677 - 12/28/09 07:20 PM (14 years, 2 months ago)

:rofl: glade im not the only one


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlinesleepyrz
Strangerwithcandy
Registered: 12/19/09
Posts: 35
Last seen: 3 years, 7 months
Re: yeast.....whats the point ? liquid culture [Re: feelfunny]
    #11733863 - 12/28/09 07:54 PM (14 years, 2 months ago)

ok im not exactly a surface thinker

i need the deep shit

like why and what part of it is nutritious

and why yeast and not something else

do ya know what i mean?

i mean i figured it contributed something in the ways of food

but what part of it does the myc like? and why maybe?


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:mushroom2: :mushdance:WHEN IT RAINS IT SHROOMS:mushdance: :mushroom2:

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Offlinesleepyrz
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Registered: 12/19/09
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Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11733873 - 12/28/09 07:55 PM (14 years, 2 months ago)

oh and ty for the replies


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:mushroom2: :mushdance:WHEN IT RAINS IT SHROOMS:mushdance: :mushroom2:

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InvisibleDoc_T
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Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11733945 - 12/28/09 08:07 PM (14 years, 2 months ago)

protein, B vitamins, what's not to like? People eat yeast for the same reasons.


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Invisiblebadman
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Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11733979 - 12/28/09 08:11 PM (14 years, 2 months ago)

Yeast it typically about 40% protein and 30% carbs and the rest various other usable nutrients fats, ions etc etc. You can use other stuff light malt extract (LME), peptone and a mixture of the above to make a liquid media or nutrient agar.

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Invisiblefeelfunny
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Re: yeast.....whats the point ? liquid culture [Re: badman]
    #11734301 - 12/28/09 09:25 PM (14 years, 2 months ago)

im still waiting for someone to try that bulk up powder from GNC its basicly the same stuff


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".

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Offlinesleepyrz
Strangerwithcandy
Registered: 12/19/09
Posts: 35
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Re: yeast.....whats the point ? liquid culture [Re: feelfunny]
    #11734995 - 12/28/09 11:33 PM (14 years, 2 months ago)

so the whole dead or alive thing

it should be dead right? so it doesnt compete with myc??

ive got:

lme
sugar in the raw packets(demerara sugar)
karo
honey
yeast


good yeast?

what do i do with it toss it in the coffee filter with everything else???

not using the karo and only a tad bit of honey  mainly  just a packet of sugar in the raw per quart jar of LC i wanna make (600ml) and a teaspoon of lme for 3 jars

so yea yeast......what do i do with it


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Offlinejc9134
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Registered: 12/05/08
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Last seen: 13 years, 7 months
Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11735235 - 12/29/09 12:40 AM (14 years, 2 months ago)

you can use yeast or not its optional, and it doesn't really matter what kind(nutritional yeast is cheaper)because even if it is alive it will be killed when you sterilize the LC/agar. there are alot of LC recipes if you look up at the top of the page for them. It seems like you are trying to complicate things, try to keep it simple. I make my LCs with just 15g of corn sugar and 15g of malt extract per 1L.

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Offlinesleepyrz
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Registered: 12/19/09
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Re: yeast.....whats the point ? liquid culture [Re: jc9134]
    #11737494 - 12/29/09 01:29 PM (14 years, 2 months ago)

nope not trying to complicate things at all

I'm trying to find what works for me and what i like best

so far Ive tried about 6 different recipes to see what i like the look of and growth characteristics of each recipe and also different methods of making the lc

so far i like
lme tsp for 3 jars ( up to 600 ml line about)
sugar in the raw packet per jar  so 3 packets
+ a dab of honey(that's precise) mine has a little pour spout  and i give it like a mississippi's worth of honey into the coffee pot not into the filter

add all that to a coffee filter in a coffee maker except the honey  add water to coffee maker that you measured in yer jars and press the on button

then i run it through a few more coffee filters placed in the neck of mah quart jars  add a stir bar  pressure cook and voila a nice lc  gets innocced and stuck on mah four plate stirrer

wanted to see if i would like lme + sugar in the raw + dab honey + yeast better or if maybe the mycelium will grow faster and more prolifically

i have two more small ideas to test also

one being a tiny bit of a multivitamin ground and added to the coffee filter

and the other is a caffeine pill just a little crushed and added to the coffee filter

maybe im crazy

maybe someones done it

i really don't care

the point is im going to try it for myself to see with my own eyes done by my own hand

ive got time
ive got money
ive got stuff
ive got space

so....."why not?" is the question, more so than  "why?"


--------------------
:mushroom2: :mushdance:WHEN IT RAINS IT SHROOMS:mushdance: :mushroom2:

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Offlinejc9134
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Re: yeast.....whats the point ? liquid culture [Re: sleepyrz]
    #11742107 - 12/30/09 11:39 AM (14 years, 2 months ago)

I love experimenting too, but there might be one problem with the yeast and that is that it might make the LC cloudy making it harder to see contams. you could try finding some liquid yeast extract and see it that works too. just an idea..let us know how it works

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InvisibleDoc_T
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Re: yeast.....whats the point ? liquid culture [Re: jc9134]
    #11742125 - 12/30/09 11:43 AM (14 years, 2 months ago)

You can't see contams in LC. You can't tell much of anything by looking at LC.


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InvisibleCordyceps
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Re: yeast.....whats the point ? liquid culture [Re: Doc_T]
    #11742358 - 12/30/09 12:27 PM (14 years, 2 months ago)

Hi Doc_t

if you can not see contams in LC how do you check it ?? inject some inside a bottle of grain ??

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InvisibleDoc_T
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Re: yeast.....whats the point ? liquid culture [Re: Cordyceps]
    #11742476 - 12/30/09 12:52 PM (14 years, 2 months ago)

Yes, that is the only way.


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Offlinejohnoutthere
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Re: yeast.....whats the point ? liquid culture [Re: Doc_T]
    #15724892 - 01/27/12 12:33 PM (12 years, 1 month ago)

I have read that sugar is not good (sucrose- complex sugar, hard to break down).

Dextrose (glucose) is a simple sugar and more readily used by the mycelium.

Yeast has B vitaminss and amino acids and peptides

Molasses has sugars, vitamins and minerals

I am experimenting with molasses and yeast extract as additional nutrients for my LC's

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OfflineBulliesAndMycology
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Re: yeast.....whats the point ? liquid culture [Re: Doc_T]
    #28664438 - 02/17/24 07:11 PM (12 days, 19 hours ago)

You can also test you LC for contaminates on agar? Noccing up grain isn't the only way. You noc agar and if the agar comes out good, THEN you noc your grains.

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Invisiblemeta_mmxxii

Registered: 08/03/23
Posts: 712
Loc: PNW
Re: yeast.....whats the point ? liquid culture [Re: BulliesAndMycology] * 1
    #28666002 - 02/18/24 07:22 PM (11 days, 19 hours ago)

why are you responding to something from 13 years ago?
Please mind the date b4 replying these people have moved on from the subject I am sure by now:shrug:


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