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Offlinenumnum59
Pro-Am Mycologist

Registered: 12/07/11
Posts: 1,189
Last seen: 1 year, 5 months
liquid culture inoculation
    #15720179 - 01/26/12 10:16 AM (12 years, 11 months ago)

Is it possible to use too much lc when inoculating. I've used 1cc on each inoculation site before but what about 1.5

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Invisibleoysterizer
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Registered: 12/20/11
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Re: liquid culture inoculation [Re: numnum59]
    #15720243 - 01/26/12 10:31 AM (12 years, 11 months ago)

Using to much lc can throw off the moisture content. 1.5 cc should be fine though. Try it and if it goes slower it got to wet.

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OfflineIcesyn
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Re: liquid culture inoculation [Re: oysterizer]
    #15720277 - 01/26/12 10:40 AM (12 years, 11 months ago)

It is understandable that you believe more LC would speed things up.

As the last dude said, moisture is where it could go wrong. Allot of people who prep grain fail to remember that before loading them up, they should be soaked and then dried correctly. Otherwise, you'll face the most common slap in the face, bacteria.

Remember, you don't need much LC to get it started. To much can ruin it and waste LC if the LC is even good at that.

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InvisibleShroom_Goon
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Registered: 08/11/11
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Re: liquid culture inoculation [Re: Icesyn]
    #15720333 - 01/26/12 10:57 AM (12 years, 11 months ago)

I recently used 5cc of LC on each grain jar. Haha I had too much of the shit and was wondering how fast it would colonize. Took about 2 weeks, including the shake, for full colonization. I was stoked. :dancer:


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:peace: :heart: :mushroom2:

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OfflineIcesyn
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Re: liquid culture inoculation [Re: Shroom_Goon]
    #15720362 - 01/26/12 11:05 AM (12 years, 11 months ago)

You were stoked, while your grains were soaked.

I'm not a gambling man. Gradz on a good LC though.

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Offlinenumnum59
Pro-Am Mycologist

Registered: 12/07/11
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Re: liquid culture inoculation [Re: Icesyn]
    #15723198 - 01/26/12 11:27 PM (12 years, 11 months ago)

one of my liquid cultures froze and the other two got really cold, do u think they are still viable?

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Invisibleoysterizer
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Re: liquid culture inoculation [Re: numnum59]
    #15723892 - 01/27/12 06:06 AM (12 years, 11 months ago)

Most cultures do not freeze well, but the ones that did not freeze should be fine. Its a good idea to do a test jar anyway.

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OfflineGalaxie500
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Re: liquid culture inoculation [Re: oysterizer]
    #15724215 - 01/27/12 09:13 AM (12 years, 11 months ago)

I keep my LC's covered with foil in the fridge. It slows 'em down (but they're definitely still growing, or my eyes are tricking me). I don't even let it warm up when I use it, straight from the fridge to the jar. I use TranscendingLife's ELME LC's and they work fantastic, DESPITE the fact I used too much ELME AND they caramelized.

As far as amounts...

I use A LOT (now, I didn't to start with). I usually used about 4cc's making BRF cakes, and I use 10cc's in my WBS jars now. My WBS colonizes in 8 days, like clockwork. It definitely makes a difference in time; I inoculate down the sides of the jars and then distribute however much is left after that all over the top of the grain, so instead of a small splotch germinating, a huge portion germinates all at once. I usually hit ~20% for the shake in about 3 days. I don't use the textbook recipe and methodology, however. I make my grains/cakes intentionally drier than specified to compensate for the extra moisture I'm adding. With BRF cakes it was fairly easy, I would essentially just subtract out at least 4cc's from the recipe. It's a little less exacting for WBS, I spread it all out over a table and let it sit for a couple hours versus letting it just drain out of the colander.

It's always a nice surprise waking up a couple days after inoculation to discover that half the sides of the jars are colonized. There definitely was a difference when I made/make BRF cakes, but there's a HUGE difference in the WBS jars.

Edited by Galaxie500 (01/27/12 09:16 AM)

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Offlinenumnum59
Pro-Am Mycologist

Registered: 12/07/11
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Re: liquid culture inoculation [Re: Galaxie500]
    #15725536 - 01/27/12 03:34 PM (12 years, 11 months ago)

Thanks galaxy

And what's the best way to make a test jar

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InvisibleInfinitys Minute
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Registered: 11/02/11
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x [Re: numnum59]
    #15726065 - 01/27/12 05:31 PM (12 years, 11 months ago)

x


--------------------
All my posts are completely fictional and are for entertainment purposes only, images I use, post or reference on this account are sourced from the internet and are not my own.

Edited by Infinitys Minute (10/04/16 01:27 AM)

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Offlinerony-pony
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Registered: 03/17/11
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Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: numnum59]
    #15726743 - 01/27/12 07:50 PM (12 years, 11 months ago)

Morning all,

I was wondering if anyone could point me in the direction of a step by step tek for making  LC from colonized rye grain? Is it correct to assume that LC's go a lot further? - how long do they last... as in how long can you take from one LC (Time-wise I mean, does the LC regenerate?)
Also is it commonly agreed that straw logs are a safer bet than laundry baskets? Cobweb seems to be a recurring problem. How can this be prevented?

Will the same LC tek work for spore syringe?

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Invisibleoysterizer
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Re: liquid culture inoculation [Re: rony-pony] * 1
    #15728312 - 01/28/12 06:42 AM (12 years, 11 months ago)

To make lc from colonized grain, simply inject jar with sterile water and shake the hell out of it. Then suck out the liquid and your ready to noc up jars. I try to use lc made like this within a week. You could squirt a little into some premade lc and grow more. My lc's that are grown in malt water will last about a year in the fridge, but many contaminate after pulling from them a couple times. I like to make several jars and plan to use all of one jar at a time. Contamination can be very hard to see in lc. The myc will regenerate in lc as long as there is still nutrition to feed it,which there wont be if pulled from a jar of grain with sterile water.
Lc can stretch a long way, you dont need much as long as you have patience. In my opinion patience is key to growing mushrooms.
Cobweb can be prevented with enough fae and lower humidity, or sprayed  with hydrogen peroxide.

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Offlinerony-pony
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Re: liquid culture inoculation [Re: oysterizer]
    #15729254 - 01/28/12 01:00 PM (12 years, 11 months ago)

That's great, thanks.

To prevent cobweb and other contams do you think using a hair dryer round to dry the edges of the straw log would help if there is no H202 at hand, until H2O2 is bought
Two weeks in and the mycellium hasn't taken full control, perhaps a not enough grain was used.

I agree with you, patience and attention to details go a very long way.

An expert is someone who has made all the mistake already! You really do learn from your mistakes

Thanks

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InvisiblePinsWellWithOthers
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Re: liquid culture inoculation [Re: rony-pony]
    #15729304 - 01/28/12 01:18 PM (12 years, 11 months ago)

.

Edited by PinsWellWithOthers (03/06/12 05:44 PM)

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Invisibleoysterizer
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Re: liquid culture inoculation [Re: rony-pony]
    #15730234 - 01/28/12 05:13 PM (12 years, 11 months ago)

Quote:

rony-pony said:
That's great, thanks.

To prevent cobweb and other contams do you think using a hair dryer round to dry the edges of the straw log would help if there is no H202 at hand, until H2O2 is bought
Two weeks in and the mycellium hasn't taken full control, perhaps a not enough grain was used.

I agree with you, patience and attention to details go a very long way.

An expert is someone who has made all the mistake already! You really do learn from your mistakes

Thanks



Not sure about the hair dryer, havent tried that before.  H2o2 just melts cobweb right before your eyes, so i never tried anything else after that.

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Invisiblegizmo1
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Re: liquid culture inoculation [Re: numnum59]
    #15731008 - 01/28/12 08:07 PM (12 years, 11 months ago)

Quote:

numnum59 said:
Thanks galaxy

And what's the best way to make a test jar



This is probably gonna be your best bet bro.
http://www.shroomery.org/forums/showflat.php/Number/15713099#15713099


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6 hole Mini Monos

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Offlinerony-pony
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Registered: 03/17/11
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Last seen: 12 years, 8 months
Re: liquid culture inoculation [Re: rony-pony]
    #15731701 - 01/28/12 11:03 PM (12 years, 11 months ago)

Where would you typically get H202, ie is it something commonly found in shops/chemists?, and what % is best to use?

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Invisibleoysterizer
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Re: liquid culture inoculation [Re: rony-pony]
    #15732556 - 01/29/12 06:36 AM (12 years, 11 months ago)

Any pharmacy or store that sells band aids. In with the first aid by rubbing alcohol. Its sold at 3% and that is a good strength for cobweb.
Usually costs about a dollar. It is sold at a higher concentrations at hydro shops but that stuff is stronger usually 30 %

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Offlinerony-pony
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Re: liquid culture inoculation [Re: oysterizer]
    #15732671 - 01/29/12 08:05 AM (12 years, 11 months ago)

Excellent, thanks very much, I'll dander to the shop now and see what I can find, does it come in liquid form? If so how is best to apply evenly? Thanks for your patience in answering my questions, I just want to try and keep mistakes to a minimum

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Invisibleoysterizer
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Re: liquid culture inoculation [Re: rony-pony]
    #15732759 - 01/29/12 08:45 AM (12 years, 11 months ago)

It comes in liquid form in a brown bottle, so as not to let any light in. Light breaks it down into water and oxygen. Put some in a spray bottle and mist affected areas. You dont want to use it anymore than is necessary to control it as it slows down mushroom myc growth as well. I have completely soak sub in h2o2 in the past when the cobweb was really bad, and it did eventually recover and fruit but it would have been faster to throw it out and make another.

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