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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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Quick help in setting a tub.
#15709791 - 01/24/12 12:08 AM (12 years, 11 months ago) |
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How long does colonization usually take when 4pnt bags of rye grain are inoculated with a straight spore syringe. (I didn't make liquid cultures) I used a 10ml spore syringe per bag and I'm worried that growth is stalled for some reason. It has only been a week but I expected something by now. I had four other previous bags that never grew and now smell sour. Probably bacterial contamination. I am not using an incubator, just a heater keeping the room warm. What do you think?
Edited by agathawiltings (01/31/12 12:53 PM)
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kansastornado
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Registered: 11/28/11
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Re: Bag Colonization taking way too long [Re: agathawiltings]
#15709839 - 01/24/12 12:21 AM (12 years, 11 months ago) |
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First way to much solution per bag. Never done them that big, but 2ml I think should be fine. Where did you get your syringes for microscopic. Who knows really, we don't know anything about your procedure. You could get a 100 replies, and they would all be a different answer. More info and pics would help out.
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RogerRabbit
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Re: Bag Colonization taking way too long [Re: kansastornado]
#15710467 - 01/24/12 06:33 AM (12 years, 11 months ago) |
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Quote:
It has only been a week
Learn patience. It takes about a week or so to see the very first patches of mycelium. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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Re: Bag Colonization taking way too long [Re: RogerRabbit]
#15721795 - 01/26/12 05:43 PM (12 years, 11 months ago) |
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we tossed out a few bags that did not colonize. Out of 5 we had one that fully colonized, another that started then stalled. Only small clumps that never seemed to spread. The 3 others never did anything except eventually start to smell sour. That was hawiian. Then we made some more grain bags, sterilized them and inoculated 6. we used a lot of spore solution to try to get a fast colonization. So far its been a week and no signs.
We started a few jars of liquid culture to hopefully speed up the time on a new set of bags we have ready.
My concern is that there just isnt enough moisture in the grain and that is where my problems are stemming from. Originally we ordered a dozen small grain bags and inoculated them with hawaiian as well those seemmed to grow slowly and then stall. Its been almost 2 months and they are still shy of being ready to use as spawn. The one bag that is ready is producing a lot of byproduct so id like to get it out of the bag and into some substrate soon.
We got a tub and coir and verm today to be ready. Im going to line the tub with panda paper, do i have the white facing out or in?
sorry if this seems scattered. Im just confuzzled.
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agathawiltings
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Registered: 11/21/11
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Re: Bag Colonization taking way too long [Re: agathawiltings]
#15721918 - 01/26/12 06:09 PM (12 years, 11 months ago) |
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at what ratio do i mix the verm and coir? should i mix it and PC in a bag?
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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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Ok so we are setting a tub today. Out of like 5 grain bags one survived and fully colonized. Plus the small pound bag we ordered and inoculated months ago, again only one fully colonized,out of 12. The others seem to be stunted and not progressing past a certain point. I dont know why only one out of each made it.
So we have a large bag and a small bag, meybe 4-5lbs of spawn.
We sterilized our 70/30 coir verm mix last night and its almost done draining.
We completed the build on our tub and its waiting. the plan is now to shower disinfect all the materials and then set it.
Is there anything we are missing?
and on a side note we have a bunch of grain bags that we inoculated with a buch of solution and now 2 weeks later no visible growth. We have been having a very tough time getting the spawn to grow for some reason.
Edited by agathawiltings (01/31/12 12:51 PM)
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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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Ok so here is the low down. We must be doing something wrong.
We have been trying to build up our spawn for about 4 months now and at then end of it only have about 3 quarts of colonized spawn. We started with pre-made sterilized 1 pound bags of rye grain and injected those with spores from our spore syringes(about a quarter of a syringe each). Those grew for a while and we were optomistic but they failed to totally colonate and became stagnant about half way through. Meanwhile we had steralized our own 2 quart bags of rye grains and injected them with similar amounts of spores. Only one of the 5 bags that we made colonized. I thought perhaps our problem was the temperature of our grow room. First we used a water heater but had to stop because of the electricity it was using. I think that this tactic dried out the spawn bags. After that we used a heating pad under our bags for a while but this also was drying out the substrate. We went back to using a water heater and it seems to be working better now on low heat. The bags have all been exposed to moderate sunlight (not much directly as we keep the curtains closed). Now we are trying to use our remaining syringes to make some liquid cultures and those seem to be working, if slowly. It has been about 2 weeks and only a little growth has occurred in the liquid cultures but we are still optomistic.
Today we are going to take the 3 quarts of spawn that did colonize and transfer it into a tub. We are using coir and vermiculite as a substrate and we intend to layer the substrate, then the spawn then a surface layer of the substrate. After that we plan to let it grow in a closet with a hanging light. If anyone has any experience they could share we would appreciate it, at this point we are quite lost as to where we are going wrong!
Edited by agathawiltings (01/31/12 01:32 PM)
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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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Ok so we set our tub. Hopefully everything works out.
We also had 7 bags of grain that we hadnt inoculated yet. I assumed the grain was too dry as the other six bags we had done before following the same method show no growth at all after a couple weeks. Because of this, we spent today cutting open the bags and boiling to try to get the moisture content right. We made 5 quart jars as well as 5 1/2 Half gallonish bags. Now we are PCing them again.
The grain looks better this time. Hopefully it works!
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MYSTIQUE
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Registered: 04/28/07
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The bags don't need any heat if you use heat on them the the middle will get to hot and it lead to bactrial infection. Also don't use spore syringes to inject bags you want to use either g2g or large amounts of L.C. to speed up colonization and increase your chances of success.
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Sippin lots of mushroom tea in a tye-dye shirt
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shroomerite
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Registered: 06/09/06
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Stick with doing your own rye jars, this way you don't have to guess the water content.
I put money on your failure was caused by the heaters. Heaters dry out grains quickly. They promote bacterial growth and slow myc growth. The colonizing temps of the myc in the jars are optimal at 75-83 degrees farenhieght. Since the colonizing rye creates heat of it's own (apx 7 to 9 degrees warmer) you need to keep the ambient room temperature around 68-72 which is common room temps.
Adding more spore solution can cause slower growth as well. You only need a few spores inside. Once they germinate and mate then grow a bit, massage the bag to redistribute the colonized grain throughout the bag.
-------------------- "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR
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agathawiltings
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Registered: 11/21/11
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Last seen: 12 years, 7 months
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I thought the heating pad was what the problem was with those bags. We did get one to fully colonize though and the tub is going now. The myc seems to be growing nicely.
I am stumped as to why the next batch that we inoculated like 2 weeks ago hasnt shown any signs of germinating.
Our liquid culture seems to be going slow, but there is some clusters of growth in them. The growth in all the jars seems to be the same thing so i assume its myc. I inoculated some re hydrated grain on the first and I think I have the water content right now.
Im just bummed I wasted all that GT spore. Its been like 2 weeks and no signs in any of them.
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