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Offlineklopshroom
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Registered: 04/29/03
Posts: 9
Last seen: 13 years, 1 month
No sterilization experiment
    #1569619 - 05/22/03 04:45 PM (13 years, 6 months ago)

I bet ur wondering y i would even try this but im gonna give it a shot. too start off with im useing a malabar culture and am slightly new yet ive read most of the shroomery. i used 1 1/3 cup verm and 1/2 cup BRF and 1/2 cup h2o and put them in a 1pint jar then i took quite a lot of Mycelium cultured (honney h2o2 bird seed water) and pored it in, the culture was allmost white :wink: soo it should go fast :grin:
so far i havent PC any materals and i have 0 contams.

been 2 days 0000.0000% contams and i can see it going allready :grin: 


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OfflineRustik
Where am I?

Registered: 04/18/03
Posts: 289
Last seen: 2 years, 17 days
Re: No sterilization experiment [Re: klopshroom]
    #1569662 - 05/22/03 05:01 PM (13 years, 6 months ago)

Um.. I don't believe this belongs in advanced, but maybe..

I don't think this will work. Been 2 days and nothing you can see as far as contamination, but it's probably there.

Sorry to break the news, that's gonna fail.


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Offlineklopshroom
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Registered: 04/29/03
Posts: 9
Last seen: 13 years, 1 month
Re: No sterilization experiment [Re: Rustik]
    #1569678 - 05/22/03 05:08 PM (13 years, 6 months ago)

might not work........ but i put a lot of h2o2 in there and its still going, it doesn't matter if it works i just had to use the culture figured it might work.....your thoughts are most welcome :grin: 


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: No sterilization experiment [Re: klopshroom]
    #1570948 - 05/23/03 01:49 AM (13 years, 6 months ago)

Who knows?  Mycomasters talked about doing thigs like this, without a pressure cooker, but I'm not sure the specifics; I never read the book.  Might be something to check out, though.

How much H2O2 did you add?  2 days is way too early to tell anything, but post your results once you find some data.  Might not work, but hey -- we all learn from stuff like this, regardless, and you never know -- it may work, too.  :wink:

--
Micro   


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Offlineklopshroom
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Registered: 04/29/03
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Last seen: 13 years, 1 month
Re: No sterilization experiment [Re: micro]
    #1574177 - 05/24/03 09:04 AM (13 years, 6 months ago)

day 4 no bad stuff that i can see the myc is going a lil


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OfflineRustik
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Registered: 04/18/03
Posts: 289
Last seen: 2 years, 17 days
Re: No sterilization experiment [Re: klopshroom]
    #1575369 - 05/24/03 11:49 PM (13 years, 6 months ago)

Quote:

myc is going a lil




You mean it's stalled? cuz if so, that's no surprise. There's probably all sorts of bacteria in there that can take hold much quicker than myc. given the proper conditions.


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Offlineklopshroom
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Re: No sterilization experiment [Re: Rustik]
    #1576047 - 05/25/03 10:44 AM (13 years, 6 months ago)

come on rustik ........ its an experiment and 5 days nothing i can see, and myc isnt stalled realy jus slower the normal.


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Invisiblejojoba
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Registered: 07/07/02
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Re: No sterilization experiment [Re: klopshroom]
    #1576067 - 05/25/03 11:18 AM (13 years, 6 months ago)

LIme mixed with the substrate would give you 2 weeks b4 contams kicked in... maybe enough time to colonize fully..


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Offlineklopshroom
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Registered: 04/29/03
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Last seen: 13 years, 1 month
Re: No sterilization experiment [Re: jojoba]
    #1578056 - 05/26/03 11:50 AM (13 years, 6 months ago)

thanks guys but i f***ed it up somehow i guess the myc recoiled and went dorment sooo i guess ill toss it........... ummm im in a bad spot i made 6 jars 5 with the mmgg and one millet the mmgg ones dont look like they have enough water??? will puting in myc honny water in to comp work? i know this belongs in beginers but i figured it would work hear.....anny help some one?


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Offlinepsilo9com
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Registered: 05/20/03
Posts: 267
Last seen: 13 years, 2 months
Re: No sterilization experiment [Re: klopshroom]
    #1579845 - 05/27/03 12:02 AM (13 years, 6 months ago)

STERILIZE MY FRIEND.


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Offlineklopshroom
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Last seen: 13 years, 1 month
Re: No sterilization experiment [Re: psilo9com]
    #1581501 - 05/27/03 02:50 PM (13 years, 6 months ago)

anny one have an answer for me?


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OfflineRustik
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Registered: 04/18/03
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Re: No sterilization experiment [Re: klopshroom]
    #1581764 - 05/27/03 04:23 PM (13 years, 6 months ago)

Quote:

thanks guys but i f***ed it up somehow i guess the myc recoiled and went dorment sooo i guess ill toss it




Umm.. yah, you didn't sterilize. :wink: The mycelium did not recoil and go dormant - a competitive bacteria or mold had already taken over and eaten out the middle of your cake, and there was no where left for the mycelium to grow.

As far as your questions are concerned, your english is very bad and you didn't supply enough details. Are the MMGG and millet jars also unsterilized? If so, toss them too, they will not colonize completely if at all.

If they ARE sterilized: If they don't 'look' like they have enough water, then wait. If the myceilum growth stalls, add a small amount of sterile water to the jars. I'd say around 5-8CCs.

Quote:

will puting in myc honny water in to comp work?




No clue what the hell you are talking about. If you mean you want to put honey water into your computer, then no, that would probably cause problems. What do you mean by comp? Compete? If the jars weren't sterilized, then they will fail and you would be wasting inoculant. If they were sterilized, and they are stalled, they could still be contaminated, in which case you would be wasting inoculant. If they stall completely for a period of time, take a tiny (TINY) smell of the jar. If there's any odd smells, toss it.

I hope you learned from this 'experiment.' You simply cannot grow cubensis without sterile technique unless it is done outside, and even then you must be sterile in order to generate spawn.


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The blue... the blue!!!


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OfflineAnnoA
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Re: No sterilization experiment [Re: Rustik]
    #1581775 - 05/27/03 04:28 PM (13 years, 6 months ago)

>If you mean you want to put honey water into your computer, then no, that
>would probably cause problems

ROFL!!!


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OfflineRaadt
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Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 8 years, 10 months
Re: No sterilization experiment [Re: klopshroom]
    #1581820 - 05/27/03 04:46 PM (13 years, 6 months ago)

well, since your experiment is done, and i highly doubt anyone will have any suggestions other than sterilize next time. I'm going to close this.


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