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Offlinezathan
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Registered: 08/22/06
Posts: 902
Loc: So long stinktown
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Re: Low temperature colonization? [Re: LayYouIn]
    #6562945 - 02/13/07 01:15 PM (17 years, 7 days ago)

Quote:

LayYouIn said:
do they really colonize that much faster at 83f compared to 75f? how much of a difference could i expect? thanks.





This is what to expect.



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Offlinefourstringbass
Canadian Chemist
Male

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Registered: 12/19/06
Posts: 140
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Re: Low temperature colonization? [Re: RogerRabbit]
    #6563083 - 02/13/07 02:01 PM (17 years, 7 days ago)

Quote:

RogerRabbit said:
The key to remember is jars colonize fine at 'normal room temperature' which 66F is not. I have some fully colonized pf jars that were inoculated 8 days ago and kept at 75F on an open shelf. I'd suggest a heater for your room over an incubator, so they don't sit in stale air. As Roadkill said, 66F is too low and will take longer to colonize.
RR




And was that a LC or a multi-spore injection?


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
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Re: Low temperature colonization? [Re: fourstringbass] * 1
    #6563295 - 02/13/07 03:20 PM (17 years, 7 days ago)

I have found little to no difference in colonization speeds between 75 and 81F.  Growth falls off rapidly at 83F and above, not 87F.

That chart above is bogus, period.  I have tried dozens of times to duplicate it and it can't be done.  It was apparently made by someone who did ONE grow with sloppy note taking, and sent the results to paul.  Growth is much slower in cold temperatures until you hit 69F, where it speeds up quite a bit until about 75F, where it remains 'flat' until 81, then is flat again until 83, where it falls off fast beginning at 84.  By 'flat' I mean there is no discernible increase or decrease in rate of growth within those ranges.  Jars will colonize as fast at 75F as they will at 80F.  I've proved this time and time again with every strain in my collection.  Growth also falls off rapidly above 84, and this is why so many new folks have problems with incubators set at 86F, and jars that 'won't colonize'.  The figures I give are substrate temperatures, not air temperatures.  The temp inside the jar is 1 to 5 degrees higher than the surrounding air, depending on where in the colonization cycle the jar is.  The heat produced falls off fast as the jar approaches full colonization.

If you live in an igloo,(or near the waterfront) by all means build an incubator, but keep it in the normal room temperature ranges for best results.  I see no reason to set one above 80F, and lots of reasons not to.  Here's a picture of one of my shelves for colonizing jars.  The substrate bags are there because I ran out of room on the other shelf.  These are in a room at normal room temperature, and exposed to light nearly all day.  I don't even put the pf jars on a top shelf where it's warmer. Of course, I had a good teacher on how to make them up, as everyone will soon know. :wink:
RR


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InvisibleLayYouIn
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Registered: 09/28/06
Posts: 4,402
Loc: Organ
Re: Low temperature colonization? [Re: RogerRabbit]
    #6563510 - 02/13/07 04:23 PM (17 years, 6 days ago)

Quote:

RogerRabbit said:
I have found little to no difference in colonization speeds between 75 and 81F.  Growth falls off rapidly at 83F and above, not 87F.

That chart above is bogus, period.  I have tried dozens of times to duplicate it and it can't be done.  It was apparently made by someone who did ONE grow with sloppy note taking, and sent the results to paul.  Growth is much slower in cold temperatures until you hit 69F, where it speeds up quite a bit until about 75F, where it remains 'flat' until 81, then is flat again until 83, where it falls off fast beginning at 84.  By 'flat' I mean there is no discernible increase or decrease in rate of growth within those ranges.  Jars will colonize as fast at 75F as they will at 80F.  I've proved this time and time again with every strain in my collection.  Growth also falls off rapidly above 84, and this is why so many new folks have problems with incubators set at 86F, and jars that 'won't colonize'.  The figures I give are substrate temperatures, not air temperatures.  The temp inside the jar is 1 to 5 degrees higher than the surrounding air, depending on where in the colonization cycle the jar is.  The heat produced falls off fast as the jar approaches full colonization.

If you live in an igloo,(or near the waterfront) by all means build an incubator, but keep it in the normal room temperature ranges for best results.  I see no reason to set one above 80F, and lots of reasons not to.  Here's a picture of one of my shelves for colonizing jars.  The substrate bags are there because I ran out of room on the other shelf.  These are in a room at normal room temperature, and exposed to light nearly all day.  I don't even put the pf jars on a top shelf where it's warmer. Of course, I had a good teacher on how to make them up, as everyone will soon know. :wink:
RR





well, in that case, my room is the perfect incubator(70f-75f).

why so many jars?  :wink:

and what's this about knowledge coming soon?


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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: Low temperature colonization? [Re: LayYouIn]
    #6563560 - 02/13/07 04:36 PM (17 years, 6 days ago)

I miss the speed my tub in tub gave me but I still use room temperature,I'm not even sure of the exact temp in there but it is a little on the chilly side and they do take their sweet time to fully colonize but I don't mind,Maybe I will set a tit up again one day when I get a better heater or a thermostat to shut it off when it hits 80 but for now I would rather wait the extra time than have that awful thing shoot to 100 degrees every time I'm not looking at it for 5 min:thumbdown:


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Offlinetypicalgangsta
Stranger

Registered: 10/10/11
Posts: 23
Last seen: 10 years, 6 months
Re: Low temperature colonization? [Re: RogerRabbit]
    #15694664 - 01/20/12 07:35 PM (12 years, 29 days ago)

Hey Roger,

Im a noob and didnt know how else to ask this question so im replying so i can hopefully get an answer.

For P.Cubensis, my temp in the terrarium is 65-67F. Ive heard that it will still fruit but will take a longer time to do so and because it takes a long time it will be more POTENT and bigger and thicker than if grown in high temps.

Is this true and will my fully colonized half pint cakes be o.k to fruit at these temps?

Thank You


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