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countchocula420



Registered: 09/24/11
Posts: 2,391
Loc: Hawaii
Last seen: 6 months, 24 days
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agar question
#15692034 - 01/20/12 09:47 AM (12 years, 5 months ago) |
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whats up guys!
i'm gonna be trying the grocery store agar tek today, i've got it all down except for the part about how much of the spore syringe i should use with each jar of agar, i'll be using half pints. i'm using re-hydrated spores in a syringe. thanks guys
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penhed
spawniac



Registered: 11/28/10
Posts: 863
Loc: holding the axis
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with spores.....less is more
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countchocula420



Registered: 09/24/11
Posts: 2,391
Loc: Hawaii
Last seen: 6 months, 24 days
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Re: agar question [Re: penhed]
#15692089 - 01/20/12 10:05 AM (12 years, 5 months ago) |
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Quote:
penhed said: with spores.....less is more
sorry dude but do you have a number or something ? .25 mL, .5?
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RogueTrippeR
Peaceful


Registered: 02/27/11
Posts: 551
Loc: PNW
Last seen: 7 years, 3 days
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shake well and add one drop
-------------------- Cluster Headache
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tripdawg420
low life with no life



Registered: 02/02/09
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a few drops
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Wing
The Eye Tyrant



Registered: 04/25/11
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Less is more. A single drop should have a bunch of potential strains.
-------------------- My Old Grow Logs
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Quote:
countchocula420 said: whats up guys!
i'm gonna be trying the grocery store agar tek today, i've got it all down except for the part about how much of the spore syringe i should use with each jar of agar, i'll be using half pints. i'm using re-hydrated spores in a syringe. thanks guys
When you say "i'll be using half pints", I think "LC" where you are talking about agar.
Are you going to put a thin layer of agar on that bottom of each jar? I might recommend the little 1/4 pint jars in this case.
Anyway, good luck!
JD
P.S. Actually, I would recommend disposable petris from Cyanmar.
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
Edited by Javadog (01/20/12 10:28 AM)
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countchocula420



Registered: 09/24/11
Posts: 2,391
Loc: Hawaii
Last seen: 6 months, 24 days
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Re: agar question [Re: Javadog]
#15692225 - 01/20/12 10:37 AM (12 years, 5 months ago) |
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I was going to use the grocery store no pour tek. yeah i know they use 1/4 pint jars in the tek, but i asked in a thread if the 1/2 pint jars will work for this and everyone said yes. yeah i'm gonna leave a thin layer of agar on each jar.
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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I get it. Right on.
Good luck,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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countchocula420



Registered: 09/24/11
Posts: 2,391
Loc: Hawaii
Last seen: 6 months, 24 days
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Re: agar question [Re: Javadog]
#15692258 - 01/20/12 10:44 AM (12 years, 5 months ago) |
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Thanks JD,
hey so just a couple drops from a syringe should be good? maybe .25 mL?
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Yes.
I would streak the inoculant about a tad.
Good luck,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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RogueTrippeR
Peaceful


Registered: 02/27/11
Posts: 551
Loc: PNW
Last seen: 7 years, 3 days
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Re: agar question [Re: Javadog]
#15692346 - 01/20/12 11:05 AM (12 years, 5 months ago) |
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One drop will do it. More and you are going to have extra water in the jar.
Better yet, just go spores to agar.
-------------------- Cluster Headache
Edited by RogueTrippeR (01/20/12 11:06 AM)
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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This is true.
(I kinda brain-farted your specific number)
How much spore syringe solution do I put on an agar dish?
As little as possible, frankly.
There, fixed.
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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countchocula420



Registered: 09/24/11
Posts: 2,391
Loc: Hawaii
Last seen: 6 months, 24 days
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Re: agar question [Re: Javadog]
#15692857 - 01/20/12 01:19 PM (12 years, 5 months ago) |
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I get it. I will PM you JD if i have any questions with it ok? Thanks dude
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Any time.
If we find some break-through, then we will share it here.
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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