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GroosBrewz
Fungus Freak



Registered: 01/10/11
Posts: 123
Last seen: 3 years, 10 months
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Does this Shiitake LC look right?
#14076242 - 03/06/11 03:54 PM (12 years, 11 months ago) |
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I inoculated some LC (karo) with a shiitake wedge about 5 days ago.. I have these little fuzzy balls floating around and I am wondering if this is normal for shiitake? My oyster LC looks normal (stringy and mucus like) while these literally look like little balls.. I know the pic aint great but maybe some can tell me if this is normal or not.. Thanks
-------------------- "It's better to have it and not need it than need it and not have it" -- Captain Call "American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle
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cloudwalker
Sonic Tinkerer



Registered: 07/04/10
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Loc: in the studio
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Re: Does this Shiitake LC look right? [Re: GroosBrewz]
#14076392 - 03/06/11 04:32 PM (12 years, 11 months ago) |
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give is another week. My shiitake LCs take 2 weeks to fully bloom. (oysters only 5-7 days by comparison)
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pepper
SawDust Block Junkie



Registered: 10/31/10
Posts: 646
Loc: British Columbia
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Re: Does this Shiitake LC look right? [Re: cloudwalker]
#14077325 - 03/06/11 07:17 PM (12 years, 11 months ago) |
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Here is my Shiitake LC. Its just water with one petri dish of Shiitake ground up.
 
Yeah it does take some time to grow...
I see that you have posted in this thread... https://www.shroomery.org/forums/showflat.php/Number/13696725/fpart/1/vc/1
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Edited by pepper (03/06/11 07:28 PM)
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MonkeyKnifeFight
Stranger


Registered: 06/08/10
Posts: 772
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Re: Does this Shiitake LC look right? [Re: pepper]
#14078120 - 03/06/11 09:23 PM (12 years, 11 months ago) |
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Seems kind of important to recognize the different kinds of LC. Pepper it sounds like you're talking about taking a petri and blending it in a sterile blender with some sterile water. I could be wrong but I don't think most folks around here have an autoclavable blender setup.
A sugar based LC to expand mycelium in is very different and more prone to contams and other issues.
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pepper
SawDust Block Junkie



Registered: 10/31/10
Posts: 646
Loc: British Columbia
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Quote:
MonkeyKnifeFight said: Seems kind of important to recognize the different kinds of LC.talking about taking a petri and blending it in a sterile blender with some sterile water. I could be wrong but I don't think most folks around here have an autoclavable blender setup.
1. Do you have a blender? 2. Do you have regular mouth size jars? Pint would be nice. 3. Unscrew the bottom of the blender so you only have the metal blades and metal disk that the blades are attached to. 4. This should fit into a regular mouth jar ring. 5. Your golden with your cheap 500ml Eberbach Container that didn't cost you 2 big ones...
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MonkeyKnifeFight
Stranger


Registered: 06/08/10
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Re: Does this Shiitake LC look right? [Re: pepper]
#14078508 - 03/06/11 10:29 PM (12 years, 11 months ago) |
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Holy shit pepper I'm totally not getting what you're describing but it sounds cool. What's the process? You take the blades out of the blender and PC them inside the pint jar? With the water? What's driving the blending? I like the promise of simple sterile blending but I'm not getting what you're talking about. Can you share more details?
Thanks!
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pepper
SawDust Block Junkie



Registered: 10/31/10
Posts: 646
Loc: British Columbia
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Re: Does this Shiitake LC look right? [Re: pepper]
#14078530 - 03/06/11 10:32 PM (12 years, 11 months ago) |
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Quote:
pepper said: Here is my Shiitake LC. Its just water with one petri dish of Shiitake ground up.
  Yeah it does take some time to grow... https://www.shroomery.org/forums/showflat.php/Number/13696725/fpart/1/vc/1
It's all in that thread that is posted in the quote above.
Grab a small regular mouth jar with the jar ring. 
Grab your blender blade assembly.
Put it together like the pictures above.
Edited by pepper (03/06/11 11:44 PM)
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themycoman
Mycologist



Registered: 11/13/10
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Re: Does this Shiitake LC look right? [Re: pepper]
#14079900 - 03/07/11 07:41 AM (12 years, 11 months ago) |
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I'm really loving these cheap alternatives to a eberbach.
Will definitely make one for myself when I start working with agar.
It seems it would be much faster than having to grow out a grain LC like I'm doing now.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Does this Shiitake LC look right? [Re: GroosBrewz]
#14080012 - 03/07/11 08:23 AM (12 years, 11 months ago) |
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Make sure to test the LC before moving onto larger projects. I place a drop or two of the LC onto an agar plate and you can easily see within a day or two what exactly is going on.
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pepper
SawDust Block Junkie



Registered: 10/31/10
Posts: 646
Loc: British Columbia
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Quote:
EvilMushroom666 said: Make sure to test the LC before moving onto larger projects. I place a drop or two of the LC onto an agar plate and you can easily see within a day or two what exactly is going on.
That is a good point to remember. I have read threads where people have inoculating large amounts of grain only to find out that their LC was in infected. All the grain and hard work was lost.
So people that make LC's always assume that it is infected until they test it, being on agar or in a grain jar.
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GroosBrewz
Fungus Freak



Registered: 01/10/11
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Re: Does this Shiitake LC look right? [Re: pepper]
#14086214 - 03/08/11 11:44 AM (12 years, 11 months ago) |
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So, does shiitake LC form these little "balls? before growing into strand-like mycelium? I used a wedge from a petri.
Also on a related subject, can I use a LC to start a new LC? I would like to shoot some of my good oyster LC into a new LC- will it grow?
Thanks all!
-------------------- "It's better to have it and not need it than need it and not have it" -- Captain Call "American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle
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Momofo
Stranger than you



Registered: 01/06/10
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Re: Does this Shiitake LC look right? [Re: GroosBrewz]
#14087853 - 03/08/11 05:11 PM (12 years, 11 months ago) |
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I have a shiitake LC that looks similar to yours.... it grew out on agar OK when I tested it......but was somewhat wispy... and did pretty much the same thing when tested on grain
it came from sporeworks
to answer your other question....Yes...you can use a good LC and expand it to another but I personally wouldnt use the second one to start a third(and so on and so on)
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pepper
SawDust Block Junkie



Registered: 10/31/10
Posts: 646
Loc: British Columbia
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Re: Does this Shiitake LC look right? [Re: Momofo]
#14088385 - 03/08/11 07:02 PM (12 years, 11 months ago) |
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Quote:
GroosBrewz said: So, does shiitake LC form these little "balls? before growing into strand-like mycelium? I used a wedge from a petri.
Also on a related subject, can I use a LC to start a new LC? I would like to shoot some of my good oyster LC into a new LC- will it grow?
Thanks all!
Quote:
Momofo said: I have a shiitake LC that looks similar to yours.... it grew out on agar OK when I tested it......but was somewhat wispy... and did pretty much the same thing when tested on grain
it came from sporeworks
to answer your other question....Yes...you can use a good LC and expand it to another but I personally wouldnt use the second one to start a third(and so on and so on)
My little balls grew into bigger balls of mycelium. Use the mother jar to make clones jars.
Edited by pepper (03/08/11 07:05 PM)
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GroosBrewz
Fungus Freak



Registered: 01/10/11
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Last seen: 3 years, 10 months
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Re: Does this Shiitake LC look right? [Re: pepper]
#14088531 - 03/08/11 07:26 PM (12 years, 11 months ago) |
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Hey all,
A quick update- I came home tonight to find the little "balls" starting to vaguely resemble mycelium.. So that's good news! However, the bad news is that the LC is starting to look slightly cloudy.. My oyster LC, which kicked ass, was never cloudy.. Always crystal clear.. Should I be worried?
-------------------- "It's better to have it and not need it than need it and not have it" -- Captain Call "American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Does this Shiitake LC look right? [Re: GroosBrewz]
#14089342 - 03/08/11 09:38 PM (12 years, 11 months ago) |
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In MY experience with LME & Dextrose LC`s cloudyness after inoculation usually means bacterial contamination. Take a drop or two to agar to confirm.
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GroosBrewz
Fungus Freak



Registered: 01/10/11
Posts: 123
Last seen: 3 years, 10 months
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Plan on dropping onto agar tonight..
-------------------- "It's better to have it and not need it than need it and not have it" -- Captain Call "American beer is like making love in a canoe- it's f@#%ing close to water!" Eric Idle
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OICU812
NC Tree Farm owner


Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
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There's a tip I can use...simple too! The Agar petri will proof a LC batch well. Thanks
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