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AbbeyRoad



Registered: 12/13/11
Posts: 419
Last seen: 6 months, 17 days
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Ready for GLC\MYC H2O Extraction?
#15684783 - 01/18/12 05:53 PM (12 years, 5 months ago) |
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Have a 1\2 of a 1\2 pint of rye going to produce my myc water for next inoculation. Its been about 1.5 weeks since it was noc'd and everything was 100% white with healthy myc covering every grain. So I figured it was done and got ramped up to do the extraction.
I had read that someone recommended shaking the jar before injected the water was a good idea - which I went ahead and did. But, now that I gave it a good solid shaking to break it all apart much more of the brown from the grain is exposed and it now only looks 15% colonized if you compare brown(rye)to white(myc). Should I just extract now or wait for it to recolonize and get to that 100% post-shake? I swear it looked 100% before I shook it ...
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Ready for GLC\MYC H2O Extraction? [Re: AbbeyRoad]
#15684791 - 01/18/12 05:55 PM (12 years, 5 months ago) |
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You want to inject your water first and then shake, so that all the mycelium that is knocked loose and disturbed gets caught in the water creating your mycelium slurry or grain LC.
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Tangich


Registered: 10/28/09
Posts: 8,723
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When you shake and break it up, the grain looks brown and uncolonized, but is in fact fully colonized and covered with mycelium.
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UnnamedGrower
The AMUiest



Registered: 04/17/11
Posts: 7,146
Loc: I'll be where I'm at
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Quote:
EvilMushroom666 said: You want to inject your water first and then shake, so that all the mycelium that is knocked loose and disturbed gets caught in the water creating your mycelium slurry or grain LC.
QFT
You will still be fine to do the GLC though. Just shoot the water into the jar give it another shake and proceed with the GLC
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Ready for GLC\MYC H2O Extraction? [Re: Tangich]
#15684815 - 01/18/12 06:02 PM (12 years, 5 months ago) |
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Quote:
Tangich said: When you shake and break it up, the grain looks brown and uncolonized, but is in fact fully colonized and covered with mycelium.

I misunderstood what you were saying it appears.
I am sure if you inject your water now, shake and pull you will still get a mycelium slurry, but I would think that it would not be as concentrated as if you did it the other way around.
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AbbeyRoad



Registered: 12/13/11
Posts: 419
Last seen: 6 months, 17 days
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Re: Ready for GLC\MYC H2O Extraction? [Re: AbbeyRoad]
#15684874 - 01/18/12 06:14 PM (12 years, 5 months ago) |
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Thanks for the fast reply folks - guess I have just one more point of clarity if you would tho...
So seeing that I've already done the shake - should I wait a couple more days for it to recolonize then proceed or just proceed now? Im patient enough to wait equivalent to the shelf life of rye quarts that I PC'd today . ??
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Ready for GLC\MYC H2O Extraction? [Re: AbbeyRoad]
#15684903 - 01/18/12 06:19 PM (12 years, 5 months ago) |
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I can not give you personal experience as far as injecting water after the shake...I personally might wait a few days and then do it the other way around.
As for the shelf life of grain jars, it varies from grain to grain, and also your filter material/size of hole comes into play...but I say most grain jars will easily be good for 2-4 weeks if sterilized properly, I have used grain jars that had been sitting for 2 months before with ok results, I just made sure to use a little more shiitake LC then normal due to the grain drying out a small bit.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 months
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Quote:
EvilMushroom666 said: You want to inject your water first and then shake, so that all the mycelium that is knocked loose and disturbed gets caught in the water creating your mycelium slurry or grain LC.
I disagree. When I wrote the original writeup on this(which Agar then made into a pictorial tek) I had been experimenting with the technique for quite some time. It's best to shake well first to break up the grains because this allows much more water to be added. Then, you shake again to liberate the mycelium from the grains into the water.
After pouring off the water, the grains can still be used to inoculate a bulk substrate.
To the OP, 1/2 of a half pint is not much mycelium or inoculant. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Ready for GLC\MYC H2O Extraction? [Re: RogerRabbit]
#15685521 - 01/18/12 08:25 PM (12 years, 5 months ago) |
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Fair enough RR,
I have been doing it the other way around for all my edible grain LC projects and I did notice that it was a bit tricky sometimes, I figured that having the water in the vessel before shaking would liberate more of the mycelium straight into solution.
Learn something new everyday, if it works it works, thanks for chiming in
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AbbeyRoad



Registered: 12/13/11
Posts: 419
Last seen: 6 months, 17 days
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Re: Ready for GLC\MYC H2O Extraction? [Re: AbbeyRoad]
#15689265 - 01/19/12 05:29 PM (12 years, 5 months ago) |
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Thanks Evil and RR,
For what its worth I went ahead with hydration - another shake - extraction... Seems like I got plenty of myc bits extracted in the water IMO. Went ahead and pulled about 60cc worth. Seeing that I was in my GB already, I just went ahead and shot a couple test jars... so Ill soon enough if I got this beat, or if I was beaten
Stay tuned
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