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OfflineMerkel
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Registered: 01/18/12
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4 days after using liquid culture to inoculate and no growth
    #15682570 - 01/18/12 09:34 AM (12 years, 5 months ago)

Hi all,

Great site and community.

It's been 4 days since I inoculated 4 BRF jars with my liquid culture and there has been zero signs of growth. Should I assume that the liquid culture has failed? The jars are in an incubator at the correct temperature.

Thanks for any help

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OfflineJonow
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15682611 - 01/18/12 09:46 AM (12 years, 5 months ago)

Sometimes LC takes awhile. I've had LC take a week and a half to show growth, but when it started it finished incredibly fast.

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OfflineShamrocker
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Jonow]
    #15682687 - 01/18/12 10:08 AM (12 years, 5 months ago)

If you had growth in your LC and you sucked it up into the syringe, something should start growing in the jars. Hopefully myc! Lol. Just give it time.

Good Luck!

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Shamrocker]
    #15682781 - 01/18/12 10:33 AM (12 years, 5 months ago)

how did you make your LC?  4 jars is a good test to see how your LC turned out :thumbup:  give it time.


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InvisibleLeopardMan
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Shamrocker]
    #15682786 - 01/18/12 10:35 AM (12 years, 5 months ago)

Also, incubators are not only useless for this hobby but sometimes they can even be detrimental. Let your jars colonize at normal room temperature.


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You have to die a few times before you can really live.

-Charles Bukowski-

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OfflineMerkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: LeopardMan]
    #15683502 - 01/18/12 01:27 PM (12 years, 5 months ago)

Thanks for the responses guys.

Rogue - I used Otto's liquid culture tek.

LeopardMan - Is that the case? I heard they colonise quite slowly, I'm in the UK and it's pretty cold.

Cheers

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15683679 - 01/18/12 02:06 PM (12 years, 5 months ago)

Spore LC is a risky way to go.  If you want to do LC I suggest making a grain LC.

I agree with LeopardMan about incubators.  It would have to be below 60F before I would worry about incubating.

Did you see myc growth.  You are probably good.  It's just a matter of time now :smile:


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Edited by RogueTrippeR (01/18/12 02:08 PM)

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InvisibleLeopardMan
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15683794 - 01/18/12 02:32 PM (12 years, 5 months ago)

Quote:

RogueTrippeR said:
Spore LC is a risky way to go.  If you want to do LC I suggest making a grain LC.

I agree with LeopardMan about incubators.  It would have to be below 60F before I would worry about incubating.

Did you see myc growth.  You are probably good.  It's just a matter of time now :smile:




Good advices here :super:


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You have to die a few times before you can really live.

-Charles Bukowski-

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OfflineMerkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: LeopardMan]
    #15683826 - 01/18/12 02:39 PM (12 years, 5 months ago)

I have seen no sign of growth in the LC inoculated BRF jars, but I had some jars set up before that which I started from Spores and they're fully colonised.

Thanks for the tips!

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15683835 - 01/18/12 02:40 PM (12 years, 5 months ago)

Did the LC have MYC in it?


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OfflineMerkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15683894 - 01/18/12 02:53 PM (12 years, 5 months ago)

Yeah, well I assume it's myc. It seems to have stalled growing after about 1.5 weeks though

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15683950 - 01/18/12 03:05 PM (12 years, 5 months ago)

You'll see soon :smile:


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Offlinethedoc8
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15684012 - 01/18/12 03:17 PM (12 years, 5 months ago)

Quote:

RogueTrippeR said:
Spore LC is a risky way to go.  If you want to do LC I suggest making a grain LC.

I agree with LeopardMan about incubators.  It would have to be below 60F before I would worry about incubating.

Did you see myc growth.  You are probably good.  It's just a matter of time now :smile:




Explain to the readers why spore lc is risker than grain lc.

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: thedoc8]
    #15684225 - 01/18/12 03:54 PM (12 years, 5 months ago)

I'm not the most eloquent writer to answer this.

I'll just say that with a grain LC you are able to observe the colonizing grains before using therefor you have a much better sense that what you are using is clean.

With spores it's a crap shoot.

In most cases it's best to aviod LC altogether.  They do have there place, but you will get quicker results working with grain.


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Edited by RogueTrippeR (01/18/12 03:57 PM)

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OfflineMerkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15684280 - 01/18/12 04:06 PM (12 years, 5 months ago)

I don't have a pressure cooker at the minute, and I believe I would need one for grain liquid culture... perhaps I could buy a pre sterilised bag.

Thanks

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Offlinethedoc8
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15684282 - 01/18/12 04:07 PM (12 years, 5 months ago)

Good sensible answer.

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OfflineRogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15684376 - 01/18/12 04:25 PM (12 years, 5 months ago)

Quote:

Merkel said:
I don't have a pressure cooker at the minute, and I believe I would need one for grain liquid culture... perhaps I could buy a pre sterilised bag.

Thanks




Then make a clone LC :smile:


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OfflineMerkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
    #15684412 - 01/18/12 04:32 PM (12 years, 5 months ago)

I'll read up on it, I assume it's cutting some tissue from a BRF cake or some stem and putting that in a honey/water mix

cheers

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OfflineSwwert
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
    #15684514 - 01/18/12 04:52 PM (12 years, 5 months ago)

If you had myc in your LC, you will see something grow soon. either contam or healthy myc but something will grow


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