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Merkel
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4 days after using liquid culture to inoculate and no growth
#15682570 - 01/18/12 09:34 AM (12 years, 5 months ago) |
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Hi all,
Great site and community.
It's been 4 days since I inoculated 4 BRF jars with my liquid culture and there has been zero signs of growth. Should I assume that the liquid culture has failed? The jars are in an incubator at the correct temperature.
Thanks for any help
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Jonow
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15682611 - 01/18/12 09:46 AM (12 years, 5 months ago) |
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Sometimes LC takes awhile. I've had LC take a week and a half to show growth, but when it started it finished incredibly fast.
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Shamrocker
mst3k



Registered: 02/17/11
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Jonow]
#15682687 - 01/18/12 10:08 AM (12 years, 5 months ago) |
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If you had growth in your LC and you sucked it up into the syringe, something should start growing in the jars. Hopefully myc! Lol. Just give it time.
Good Luck!
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Shamrocker]
#15682781 - 01/18/12 10:33 AM (12 years, 5 months ago) |
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how did you make your LC? 4 jars is a good test to see how your LC turned out give it time.
-------------------- Cluster Headache
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LeopardMan
Constantly changing



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Re: 4 days after using liquid culture to inoculate and no growth [Re: Shamrocker]
#15682786 - 01/18/12 10:35 AM (12 years, 5 months ago) |
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Also, incubators are not only useless for this hobby but sometimes they can even be detrimental. Let your jars colonize at normal room temperature.
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You have to die a few times before you can really live. -Charles Bukowski-
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Merkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: LeopardMan]
#15683502 - 01/18/12 01:27 PM (12 years, 5 months ago) |
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Thanks for the responses guys.
Rogue - I used Otto's liquid culture tek.
LeopardMan - Is that the case? I heard they colonise quite slowly, I'm in the UK and it's pretty cold.
Cheers
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15683679 - 01/18/12 02:06 PM (12 years, 5 months ago) |
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Spore LC is a risky way to go. If you want to do LC I suggest making a grain LC.
I agree with LeopardMan about incubators. It would have to be below 60F before I would worry about incubating.
Did you see myc growth. You are probably good. It's just a matter of time now
-------------------- Cluster Headache
Edited by RogueTrippeR (01/18/12 02:08 PM)
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LeopardMan
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15683794 - 01/18/12 02:32 PM (12 years, 5 months ago) |
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Quote:
RogueTrippeR said: Spore LC is a risky way to go. If you want to do LC I suggest making a grain LC.
I agree with LeopardMan about incubators. It would have to be below 60F before I would worry about incubating.
Did you see myc growth. You are probably good. It's just a matter of time now 
Good advices here
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You have to die a few times before you can really live. -Charles Bukowski-
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Merkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: LeopardMan]
#15683826 - 01/18/12 02:39 PM (12 years, 5 months ago) |
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I have seen no sign of growth in the LC inoculated BRF jars, but I had some jars set up before that which I started from Spores and they're fully colonised.
Thanks for the tips!
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15683835 - 01/18/12 02:40 PM (12 years, 5 months ago) |
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Did the LC have MYC in it?
-------------------- Cluster Headache
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Merkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15683894 - 01/18/12 02:53 PM (12 years, 5 months ago) |
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Yeah, well I assume it's myc. It seems to have stalled growing after about 1.5 weeks though
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15683950 - 01/18/12 03:05 PM (12 years, 5 months ago) |
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You'll see soon
-------------------- Cluster Headache
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thedoc8
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15684012 - 01/18/12 03:17 PM (12 years, 5 months ago) |
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Quote:
RogueTrippeR said: Spore LC is a risky way to go. If you want to do LC I suggest making a grain LC.
I agree with LeopardMan about incubators. It would have to be below 60F before I would worry about incubating.
Did you see myc growth. You are probably good. It's just a matter of time now 
Explain to the readers why spore lc is risker than grain lc.
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: thedoc8]
#15684225 - 01/18/12 03:54 PM (12 years, 5 months ago) |
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I'm not the most eloquent writer to answer this.
I'll just say that with a grain LC you are able to observe the colonizing grains before using therefor you have a much better sense that what you are using is clean.
With spores it's a crap shoot.
In most cases it's best to aviod LC altogether. They do have there place, but you will get quicker results working with grain.
-------------------- Cluster Headache
Edited by RogueTrippeR (01/18/12 03:57 PM)
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Merkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15684280 - 01/18/12 04:06 PM (12 years, 5 months ago) |
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I don't have a pressure cooker at the minute, and I believe I would need one for grain liquid culture... perhaps I could buy a pre sterilised bag.
Thanks
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thedoc8
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15684282 - 01/18/12 04:07 PM (12 years, 5 months ago) |
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Good sensible answer.
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RogueTrippeR
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15684376 - 01/18/12 04:25 PM (12 years, 5 months ago) |
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Quote:
Merkel said: I don't have a pressure cooker at the minute, and I believe I would need one for grain liquid culture... perhaps I could buy a pre sterilised bag.
Thanks
Then make a clone LC
-------------------- Cluster Headache
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Merkel
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Re: 4 days after using liquid culture to inoculate and no growth [Re: RogueTrippeR]
#15684412 - 01/18/12 04:32 PM (12 years, 5 months ago) |
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I'll read up on it, I assume it's cutting some tissue from a BRF cake or some stem and putting that in a honey/water mix
cheers
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Swwert
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Re: 4 days after using liquid culture to inoculate and no growth [Re: Merkel]
#15684514 - 01/18/12 04:52 PM (12 years, 5 months ago) |
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If you had myc in your LC, you will see something grow soon. either contam or healthy myc but something will grow
-------------------- Whole Brown Rice Cakes TEK
"The mushrooms are just loving you back." "Maria Sabina is a symbol of wisdom and love."
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