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OfflineTheOptical
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Registered: 12/10/11
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MY FIRST CONTAMINATION!!!
    #15678468 - 01/17/12 02:40 PM (12 years, 5 months ago)

YUCKY!

Checked on some of my jars today to find that a group of 5 that I made at the same time were all contaminated! I opened them up, in a different room from my projects, to confirm the worst. Grey mold.

Now how did all 5 get contaminated? Well these were some of the first jars that I made. I was even more of a noob then than I am now, and I packed the substrate in the jars too tightly. This meant the bottom, plus likely the center of the jars, were unable to colonize. I got the bright idea of dumping the contents of the jars and repacking the same contents into the same jars, only this time loosely. Lo and behold, a few weeks later, 100% contamination!

A noob's gotta learn some way I guess. I'm not even upset, I have many more jars, and I know exactly why these 5 are contaminated and the jars I did correctly are not.

Needless to say, these jars are now in the garbage.

Check out my sweet contamination pic.


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OfflineKizzle
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Registered: 08/30/11
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Re: MY FIRST CONTAMINATION!!! [Re: TheOptical]
    #15678692 - 01/17/12 03:25 PM (12 years, 5 months ago)

You dumped the contents out and repacked without re-sterilizing? :lol:


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OfflineTheOptical
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Re: MY FIRST CONTAMINATION!!! [Re: Kizzle]
    #15683833 - 01/18/12 02:40 PM (12 years, 5 months ago)

That would have killed the mycelium in the jars. I didn't want to have to reinoculate the jars; I just wanted to loosen them up so the mycelium would spread properly.

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OfflineKizzle
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Re: MY FIRST CONTAMINATION!!! [Re: TheOptical]
    #15684521 - 01/18/12 04:54 PM (12 years, 5 months ago)

I'm guessing you're using the tall half-pints. It's a common problem for the bottoms to finishes colonizing with those. A better solution though is to birth the cake and rinse off all traces of uncolonized substrate. This seems to work best if you wait until the jar starts pinning invitro as the mycelium is a bit more solid making it much easier to remove the uncolonized sub.

Uncolonized sterile substrate will contaminate instantly when exposed to unfiltered air even for a moment. So by putting it back in the jar you would inevitably wind up with mold.


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