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Offlinebakenast
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grain liquid culture question
    #15673025 - 01/16/12 10:03 AM (12 years, 5 months ago)

hi would it be ok to make my grain LC if the jar isint 100% colonized yet? its like 80-90 percent.. would the LC just consumre the rest of the nutrients in the syringe??  or is this bad idea    thanks

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OfflineWing
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Re: grain liquid culture question [Re: bakenast]
    #15673037 - 01/16/12 10:06 AM (12 years, 5 months ago)

It might work, but I would wait until it's 100% colonized so you have no doubts of it's cleanliness.


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Offlinebakenast
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Re: grain liquid culture question [Re: Wing]
    #15673049 - 01/16/12 10:09 AM (12 years, 5 months ago)

ya im just in need of some LC right now is the problem

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OfflineMontock
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Re: grain liquid culture question [Re: Wing]
    #15673051 - 01/16/12 10:09 AM (12 years, 5 months ago)

Theoretically if you keep everything stetile I don't see why this won't work, id Def wait till 100% than wait a day or two after that. Mycology and waiting go hand in hand


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Offlinek00laid
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Re: grain liquid culture question [Re: bakenast]
    #15673052 - 01/16/12 10:09 AM (12 years, 5 months ago)

Quote:

bakenast said:
would the LC just consumre the rest of the nutrients in the syringe?? 




what nutrients?

you are shooting water into the jar
shaking jar

aspirating mycelium laden water

there are no nutes besides the grains

and the grains wont fit in your syringe.


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Offlinebakenast
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Re: grain liquid culture question [Re: k00laid]
    #15673064 - 01/16/12 10:12 AM (12 years, 5 months ago)

i was wanting to shoot sterile water in my jar thats 80% colonized then suck out myceliums water after shaking i assumed nutrients on the uncolonized grain kernals would leach off in the water some..

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OfflineMontock
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Re: grain liquid culture question [Re: k00laid]
    #15673066 - 01/16/12 10:13 AM (12 years, 5 months ago)

Quote:

k00laid said:
Quote:

bakenast said:
would the LC just consumre the rest of the nutrients in the syringe?? 




what nutrients?

you are shooting water into the jar
shaking jar

aspirating mycelium laden water

there are no nutes besides the grains

and the grains wont fit in your syringe.


this is all correct, I'm pretty sure he's just asking if he cab do his glc before 100%, I don't think he has the jargon down.


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Offlinek00laid
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Re: grain liquid culture question [Re: bakenast]
    #15673078 - 01/16/12 10:15 AM (12 years, 5 months ago)

Quote:

bakenast said:
i assumed nutrients on the uncolonized grain kernals would leach off in the water some..




ehhh kind of

not really.

the reason you want to wait till 100% colonization is to look for contams

there could b a trich spore on that 10% uncolonized part.

and you'll suck up a trich spore into your lc syringe.

and then everything you shoot with that syringe will turn green


you CAN get a glc from 90%
but you dont WANT to


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Offlinebakenast
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Re: grain liquid culture question [Re: k00laid]
    #15673164 - 01/16/12 10:35 AM (12 years, 5 months ago)

ok thanks just looking for some opinions, thanks for replying :thumbup:

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InvisibleTranscendingLife
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Re: grain liquid culture question [Re: bakenast]
    #15674190 - 01/16/12 03:29 PM (12 years, 5 months ago)

koolaid - sorry brother, but I disagree. You do get a bit of the nutrients from the grains in the water when you disturb it
Then extract the water from the grains & put it back in your jar.
I've had GLCs keep colonizing after they've been put back into sterile water.
I've got 4 going ATM, actually. lol.


OP - wait till it's 100% before doing the GLC, like everyone above has said.


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