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Offlinetibberous
Stranger

Registered: 12/26/04
Posts: 1,194
Last seen: 8 months, 18 days
Sterilizing with ozone?
    #15671601 - 01/15/12 10:41 PM (12 years, 5 months ago)

Would an ozone generator be a good way to sterilize a colonization / clean room before use?

Idea is to basically make up all the substrate bags, LC's, agar trays, ect.

Then run an ozone generator for however long.

Then wait however long for the ozone to dissipate.

Ideally, you'd have something to monitor the ozone with, but ozone controllers are like $350. Could you maybe use an ORP meter (cheaper)?

http://www.amazon.com/SMS510-CONTROLLER-1000-Milwaukee-Instruments/dp/B001792SLM/ref=sr_1_6?ie=UTF8&qid=1326692244&sr=8-6

I'm thinking ozone would be a good alternative to bleach / UV. (you'd still need bleach after inoculation, since ozone kills myc, I believe)

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Offlinetibberous
Stranger

Registered: 12/26/04
Posts: 1,194
Last seen: 8 months, 18 days
Re: Sterilizing with ozone? [Re: tibberous] * 1
    #15671669 - 01/15/12 10:57 PM (12 years, 5 months ago)

Found one for $35, gotta stick your tongue on it though:

http://www.ebay.com/itm/MQ131-ozone-gas-detection-sensor-output-analog-TTL-/390370164605?pt=LH_DefaultDomain_0&hash=item5ae3dfff7d

Almost wonder if I couldn't somehow hook it up with my Radioshack electronics kit. I mean, if it would save $320, not like you need an exact ozone reading, just a general idea of when the ozone is gone and it's safe to work.

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OfflineISOfantastic
Mad Scientist Wannabe
Male


Registered: 06/23/11
Posts: 384
Loc: Firmly planted in reality
Last seen: 11 years, 5 months
Re: Sterilizing with ozone? [Re: tibberous]
    #15672087 - 01/16/12 01:36 AM (12 years, 5 months ago)

You really don't need to try to achieve "clean room" conditions.
Buy a flowhood or make a glovebox.
They're reliable, well-known techs for a reason.
Look up the guidelines for a real cleanroom, you have no chance at it.
Trust a glovebox.  It works absolute wonders for $10.

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