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Bacchus
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Registered: 10/10/06
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Reliabity of stiffened "easy" felt as filter material. An experiment.
#15666342 - 01/14/12 05:30 PM (13 years, 6 days ago) |
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My local craft store stocks "stiffened felt," but not any of the brands so far discussed. To me, the felt looks basically useless for filtration. The thickness varies wildly across the surface and the thin spots appear to be far too thin to be of any protection. You can almost read through this stuff. So before I trust any valuable spawn with stiffened felt, I'm going to test it.

Description of experiment. Short version:
- Sterilize agar in mason jars. Lids hold injection ports and the filters to be tested.
- Inject syringe through port and draw out air.
- Incubate jars
Description of experiment. Long version. Several months ago, I ran out of petri dishes and ended up using half-pint jars to get by. I still have five of these jars left over. They've been sealed up tight and totally sterile all this time. So, I'm using them for this experiment. I prepared four lids with filters and injection ports. Two of the jars use tyvek as the filter material and two use the felt in question. Because I'm trading lids on the already sterilized jars (albeit prior to re-sterilization), I'm using the fifth jar to control for my sterile technique.

I replaced each jar's lid one by one in a still air box. I summarized my sterile technique here. For the control jar, I just removed the original lid and replaced it with another unmodified metal lid. I screwed all of the rings down tight, covered the jars with foil, and sterilized at 15 PSI for 20 minutes. This should be sufficient time to ensure sterilization as I'm only sterilizing about 100ml of agar separated between five jars.
The jars are cooling inside the pressure cooker as I type this. Once the jars are cool and the agar has solidified, I will set up at my dining room table. Without taking any precautions to scrub the air, I will insert a sterile syringe into each jar through the blue injection port and slowly pull air out of the jars. The negative pressure inside will cause my dirty house air to pass through the filters and into the jars.
I will then incubate all five jars at 70 degrees Fahrenheit for 14 days. If any contaminate growth is observed on agar, then it can be concluded that the filter material on that jar is not suitable for our purposes. If the control jar shows contaminate growth, then both my sterile technique and sterilization process are invalidated and I should reevaluate my life.
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Aaron8001
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#15666378 - 01/14/12 05:45 PM (13 years, 6 days ago) |
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that's the exact same felt im in the process of trying right now, and so far so good 
there is a couple threads that make use of eco-fi felt
-------------------- My Trade List
Insanity is doing the same thing over and over again and expecting different results. -Einstein
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VC_Skittles
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Aaron8001]
#15667051 - 01/14/12 08:55 PM (13 years, 6 days ago) |
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Interesting... would be nice to see how several different homemade filters perform side by side.
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AbbeyRoad



Registered: 12/13/11
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#15667196 - 01/14/12 09:37 PM (13 years, 5 days ago) |
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Likewise - I'm used the exact same brand to make my new lids for grain and LC jars. So far so good I can also tell you I've had way better luck with the easy felt filter vs using tyvek envelopes... I hope it works cause you CANT beat $.99 for a sheet.
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Bacchus
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Posts: 914
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: AbbeyRoad]
#15667491 - 01/14/12 10:56 PM (13 years, 5 days ago) |
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I'm really looking forward to felt too. I've been using tyvek like this for years. It works just fine, but I positively hate the mess left behind by the micropore tape. If the felt performs as well as the tyvek, I'll never look back
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Living on a no-Flash diet is way easier than you think. Give it a shot.
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#15691502 - 01/20/12 04:55 AM (13 years, 16 hours ago) |
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Results?
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Bacchus
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Registered: 10/10/06
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Base Icks]
#15694144 - 01/20/12 05:27 PM (13 years, 3 hours ago) |
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I just checked on them. So far so good. All five jars are contamination free after 6 days. If they're still clean after 8 more days, I'll repeat the process with the dirtiest air that I can muster. I noticed the other day that my last slice of bread had started growing mold. So, I'm going to go outside and open the breadbag inside my dirty garbage can, disturb the mold spores, and suck more air through the filters.
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Living on a no-Flash diet is way easier than you think. Give it a shot.
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#15695378 - 01/20/12 10:13 PM (12 years, 11 months ago) |
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Cool. I jsut bought 6 sheets to take back to the UK.. It was all they had on the shelf.
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Base Icks]
#15695487 - 01/20/12 10:39 PM (12 years, 11 months ago) |
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Great experiment OP. I think that Barakanatan (sp?) has also run a few experiments on similar materials.
I recently bought a set of synthetic filter disks, but am still going to grab some of this stuff when I see it.
JD
P.S. Hey Base,
Nice to see you on the boards....so, you are headed back across the pond?
Good luck to you!
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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iwaspwnd
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Registered: 10/06/08
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#15695497 - 01/20/12 10:43 PM (12 years, 11 months ago) |
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Quote:
Bacchus said: I just checked on them. So far so good. All five jars are contamination free after 6 days. If they're still clean after 8 more days, I'll repeat the process with the dirtiest air that I can muster. I noticed the other day that my last slice of bread had started growing mold. So, I'm going to go outside and open the breadbag inside my dirty garbage can, disturb the mold spores, and suck more air through the filters. 
very interested in how this goes, I went to my craft store and they only had this brand as well, creatology.
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: iwaspwnd]
#15724609 - 01/27/12 11:11 AM (12 years, 11 months ago) |
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Update?
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lshavo
Stranger

Registered: 12/26/11
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Base Icks]
#15935908 - 03/11/12 11:58 PM (12 years, 10 months ago) |
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Quote:
Base Icks said: Cool. I jsut bought 6 sheets to take back to the UK.. It was all they had on the shelf.
i work at michaels, ask those lazy fools to check overstock!!!! lol
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rodent
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Registered: 12/27/02
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: lshavo]
#15936150 - 03/12/12 02:17 AM (12 years, 10 months ago) |
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I'd also be curious in an update.
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Bacchus
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Registered: 10/10/06
Posts: 914
Loc: ::1
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: rodent]
#15943271 - 03/13/12 06:24 PM (12 years, 10 months ago) |
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Sorry, I kind of let it go at the end. The original experiment ended with all of the jars being contaminate free. I didn't do the spore-bomb experiment because I ended up using the jars to test some spore syringes and then make "agar LCs." I had real plates poured, but the injection ports on the jars were too tempting.
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Living on a no-Flash diet is way easier than you think. Give it a shot.
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jefffree
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Registered: 12/01/12
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: Bacchus]
#17329147 - 12/03/12 08:33 PM (12 years, 1 month ago) |
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Just curious if any of you guys who used the creatlogy felt still use it or have any stronger opinion on how effective it may be. Just nocced my first half dozen jars using creatology filters and rtv. How important is a clean environment while colonizing wbs w/ stiffened felt filters? Any idea?
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ps118
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Registered: 10/05/12
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: jefffree]
#17329260 - 12/03/12 08:47 PM (12 years, 1 month ago) |
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Worked great for my jars, currently experimenting using it as the soul method of FAE for my monotub about 3 days from being put into fruiting.
Had only 1 jar of 18 fail, and it was likely due to an error on my part. I used identical lids for LC's.
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jefffree
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Registered: 12/01/12
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: ps118]
#17329309 - 12/03/12 08:54 PM (12 years, 1 month ago) |
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Did you use creatology brand? And what do you think the error on your part was? I'm questioning a few steps in my process... :/ I am only 24 hours after ms injection though, so we'll see...
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ps118
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Last seen: 8 years, 10 months
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Re: Reliabity of stiffened "easy" felt as filter material. An experiment. [Re: jefffree]
#17329333 - 12/03/12 08:57 PM (12 years, 1 month ago) |
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Quote:
jefffree said: Did you use creatology brand? And what do you think the error on your part was? I'm questioning a few steps in my process... :/ I am only 24 hours after ms injection though, so we'll see...
Yes, I believe it was creatology eco-fi. The stiffened felt.
I made a mistake with one jar and didn't get a good bead of RTV sealant around all four edges of the felt and then dust got on the lid.
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