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OfflineFungus-Amongus
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Registered: 08/26/11
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Last seen: 11 years, 10 months
GLC Questions
    #15665849 - 01/14/12 03:24 PM (12 years, 5 months ago)

So I have a few questions regarding making a GLC. I would be following Agar's tek since it seems to be the only one I can find. I understand that using a grain jar is the ideal way to do this but what if you have ~1ml left in an LC syringe, could that colonize into 500ml of myc water? Also when using a grain jar for GLC, Agar uses a 60ml syringe with an 18g needle; what if you have a 20ml with a 16g and an 18g may not fit through the lid, will a 16g suffice? And how much water actually needs to be aspirated into the grain jar and then back out of it into the jar for myc water? Agar uses ~160ml however that's about 8 transfers in and 8 out; would you flame sterilize the needle each time you go from one jar to the next? I.E. Flame, Stick needle in jar to get water, take out, Flame, stick needle in grain jar to aspirate water, take out, flame... Rinse repeat? Agar mentions nothing about flaming the needle.

Thank you.


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Anything I say or do on here is all made up and fictitious. Any pictures I may have posted or thoughts and concerns I may have shared are not my own but those of others many years prior.

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OfflineISOfantastic
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Re: GLC Questions [Re: Fungus-Amongus]
    #15665918 - 01/14/12 03:39 PM (12 years, 5 months ago)

Always flame sterilize or wipe your needle down with an iso-soaked paper towel between inoculations of any kind, it prevents cross-contamination.

Honestly, GLCs are misleading, contam-prone, and if you don't have a really good eye for it, it's easy to mistake the white mycelium of trich and other molds for your cubensis mycelium.
I'd recommend looking at the link for LC in my sig.
Very simple, very very effective.
:thumbup:

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OfflineFungus-Amongus
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Re: GLC Questions [Re: ISOfantastic]
    #15666125 - 01/14/12 04:30 PM (12 years, 5 months ago)

This is a bummer, I was just about to put water in the jars and PC them. How long is a syringe sterile? Until the plunger is pulled back or the container it is in is cracked open?

Also I assume PC the jars for 30 min and then let cool? How cool?

EDIT: Also most brew stores are closed ATM and I'd really rather complete this task tonight. Grocery stores carry it?


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Anything I say or do on here is all made up and fictitious. Any pictures I may have posted or thoughts and concerns I may have shared are not my own but those of others many years prior.

Edited by Fungus-Amongus (01/14/12 04:33 PM)

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OfflineFungus-Amongus
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Re: GLC Questions [Re: Fungus-Amongus]
    #15666620 - 01/14/12 06:45 PM (12 years, 5 months ago)

Anyone?


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InvisibleUnnamedGrower
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Registered: 04/17/11
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Re: GLC Questions [Re: Fungus-Amongus]
    #15666908 - 01/14/12 08:14 PM (12 years, 5 months ago)

GLC's work well IME.  LC's you need to shoot a test jar to make sure its clean with a GLC you know your syringe is clean.  I found the grain will suck up 10-30ml of water before I was able to draw any up.  This is totally going to vary grower to grower.  A larger gauge needle is fine.  You should flame sterilize in between each transfer

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669057 - 01/15/12 10:43 AM (12 years, 5 months ago)

Awesome thank you, as far as making myc water using a LC syringe?


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InvisibleUnnamedGrower
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669074 - 01/15/12 10:47 AM (12 years, 5 months ago)

Quote:

Fungus-Amongus said:
myc water using a LC syringe?



Huh?
I know of people injecting elme water into a glc to make it grow some more.  What do you mean by myc water and lc syringe?

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669182 - 01/15/12 11:18 AM (12 years, 5 months ago)

Turning ~1ml of LC syringe into Myc water?


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Offlineundeadninja2
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669277 - 01/15/12 11:44 AM (12 years, 5 months ago)

I think he means knocking up more LC with remaining LC in syringe

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OfflineWing
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Re: GLC Questions [Re: undeadninja2]
    #15669309 - 01/15/12 11:51 AM (12 years, 5 months ago)

To make a GLC you need to get the mycelium off the grains, hence the term Grain Liquid Culture. If you're not working with grains then it's not a GLC.


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OfflineFungus-Amongus
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Re: GLC Questions [Re: undeadninja2]
    #15669319 - 01/15/12 11:52 AM (12 years, 5 months ago)

^^ Yes but with just water, no dextrose or anything like that.

When do syringes become un-sterile? When you break the seal on their container or when you actually move the plunger?


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OfflineWing
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669338 - 01/15/12 11:56 AM (12 years, 5 months ago)

Well you could use dextrose or malt. That would be like a GLC+ LMAO


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InvisibleUnnamedGrower
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669361 - 01/15/12 12:01 PM (12 years, 5 months ago)

GLC - Water containing myc.  This is made by injecting water into a fully colonized grain jar shaking and drawing the water back up.  A BRF slurry could be considered a GLC.

LC - Water and some sort of sugar.  This is made by sterilizing sugar water and inoculating it.  This will grow and can be used to inoculate.  I say sugar water but sugar isnt a good option.  I personally like ELME, but you can use honey, karo. SITR among other things.

I think the sterility of the syringe isnt broken til the plunger is moved but im not sure

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669399 - 01/15/12 12:11 PM (12 years, 5 months ago)

So can you make a "GLC" with a LC syringe? Instead of the traditional way?


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Anything I say or do on here is all made up and fictitious. Any pictures I may have posted or thoughts and concerns I may have shared are not my own but those of others many years prior.

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InvisibleUnnamedGrower
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669413 - 01/15/12 12:16 PM (12 years, 5 months ago)

What do you mean by lc syringe?  No you cant make a glc from an lc syringe.  You make a glc from a fully colonized jar.  You need to grow an lc

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669439 - 01/15/12 12:22 PM (12 years, 5 months ago)

So the water just basically suspends the myc in a GLC? If I want to turn this 1ml of LC left in the syringe into say 500ml, I suppose I'm going to need dextrose?


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InvisibleUnnamedGrower
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669479 - 01/15/12 12:29 PM (12 years, 5 months ago)

dex isnt a good option.  You could either inoculate a jar with whats left and make a glc or make an lc with honey karo elme sitr or something

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669512 - 01/15/12 12:37 PM (12 years, 5 months ago)

Hmm I suppose I'll just do another jar with it instead. Are GLCs pretty much fail safe as long as there is flame sterilization while transferring the water to the grain and vice versa?


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InvisibleUnnamedGrower
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Re: GLC Questions [Re: Fungus-Amongus]
    #15669578 - 01/15/12 12:52 PM (12 years, 5 months ago)

Nothing in mycology is fail safe.  The best you can get is a flow hood and you still encounter contams with that.  If your careful you should be alright

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OfflineFungus-Amongus
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Re: GLC Questions [Re: UnnamedGrower]
    #15669614 - 01/15/12 01:01 PM (12 years, 5 months ago)

Of course, I know that sterility is key here. Does the Myc actually grow in the water? Or do you need the same amount of myc as water?


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Anything I say or do on here is all made up and fictitious. Any pictures I may have posted or thoughts and concerns I may have shared are not my own but those of others many years prior.

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