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Offlineslugtree22
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Registered: 10/30/08
Posts: 15
Last seen: 8 years, 2 months
possible monokaryotic growth
    #15662233 - 01/13/12 07:17 PM (12 years, 1 month ago)

Had some old woodlover prints, so a couple of months ago I busted out the autoclave and made some 6 plates.  Only one plate had some germination, and am concerned that it was from only one spore.  After a couple of transfers, I now have some grain jars going.  The growth is NOT rhizomorphic.  I thought it might become so after a few transfers - can't recall where I read that.  So a couple of questions...

Is there any way to tell?  I don't have a microscope, but do have a 30x loupe.  I would be a shame to get a big healthy outdoor patch going, only to have it not able to fruit!

Could I germ some more spores, and then allow this growth to grow into what I've already propagated? 

I had lots of success expanding woodlover myc (sterile - agar to grain to woodchips) about 6 years ago when I tried it.  Was never sure if my sterile spawn contributed anything, as the outdoor patch was going pretty well with wild myc that I transferred there.  Both cyans and cyclones, but the cyclones pretty much took over after a few years.  Don't have access to that patch anymore, so am looking to start anew!

Any tips?  I have only a small amount of wild myc right now, and am in the process of expanding that.  My question is about only the sterile, possibly monokaryotic mycellium question though....


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OfflineRogerRabbitM
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Re: possible monokaryotic growth [Re: slugtree22]
    #15662319 - 01/13/12 07:38 PM (12 years, 1 month ago)

Not all dikaryotic mycelium is rhizomorphic.  Monokaryotic mycelium is tangled, looking much like mold, and for lack of a better term, a black person's hair.  Dikaryotic mycelium on the other hand is usually linear, whether it's rhizomorphic or not.
RR


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Offlineslugtree22
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Registered: 10/30/08
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Last seen: 8 years, 2 months
Re: possible monokaryotic growth [Re: RogerRabbit]
    #15662931 - 01/13/12 10:04 PM (12 years, 1 month ago)







Some pics.  Seems not to be linear.


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Offlineslugtree22
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Registered: 10/30/08
Posts: 15
Last seen: 8 years, 2 months
Re: possible monokaryotic growth [Re: slugtree22]
    #15662938 - 01/13/12 10:06 PM (12 years, 1 month ago)

Thanks for the help, btw!


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Offlineslugtree22
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Registered: 10/30/08
Posts: 15
Last seen: 8 years, 2 months
Re: possible monokaryotic growth [Re: slugtree22]
    #15664988 - 01/14/12 11:47 AM (12 years, 1 month ago)

Suppose it might not be mycellium at all?  It seems to smell like it....


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