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Offlinezamora333
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Need an Opinion on my LC pics
    #15646875 - 01/10/12 07:34 PM (13 years, 10 days ago)

These are a week in and I was a bit concerned because from what most people say it only takes a week for the to fully colonize and these def don't look fully colonized. Just need an opinion if they look like there on the right track or if I should just trash them and try a new one. There at 77degrees in a dark place. Any feedback or advice would be much appreciated as this is my first attempt.



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All said and done are not my words, all pictures are fictional, all questions are rhetorical. In fact this is an optical illusion.

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Invisiblevanthonyv
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Re: Need an Opinion on my LC pics [Re: zamora333]
    #15649526 - 01/11/12 10:40 AM (13 years, 9 days ago)

I'll chime in to get this started. What LC media did you use and at what percent? What type of lid do you have on your jar? and is it a Clone tissue sample? (as I suspect) If so did you highlight that green dot to the picture or is it just the camera lighting?

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Invisiblevanthonyv
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Re: Need an Opinion on my LC pics [Re: vanthonyv]
    #15649535 - 01/11/12 10:41 AM (13 years, 9 days ago)

Oh and if it's a clone tissue sample a healthy LC should look like a white puffy cloud around the tissue for starters.

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OfflineIcesyn
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Re: Need an Opinion on my LC pics [Re: vanthonyv]
    #15649548 - 01/11/12 10:44 AM (13 years, 9 days ago)

Allot of contams are microscopic. Allot of people post LC (even I did) asking it it looks fine. The fact is, you'll never know until you use it.

How is that on GE? Most people leave the jar half empty for GE. I'm not even sure if you can so much as shake that one to mix it up.

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Re: Need an Opinion on my LC pics [Re: zamora333]
    #15649550 - 01/11/12 10:44 AM (13 years, 9 days ago)

Hello,

Well, the primary useful indicator is the clarity of the LC.

Clear does not mean clean, but murky usually means contamed.

After a week, unless we are talking multi-spore (and really even then),
you would want to be seeing growth, and that the LC was becoming clearer
and less colored.

So, it is impossible to say for certain, I think that that LC has problems.

Take care,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Offlinezamora333
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Re: Need an Opinion on my LC pics [Re: Javadog]
    #15655432 - 01/12/12 01:53 PM (13 years, 8 days ago)

Thanks I appreciate all the feedback. I used honey and its been about two weeks and this is all I got. I also used a clone from a spore syringe. And I bought a lid that already has a filter point for air exchange. And the thing inside is a marble I sterilized to break up the myc. Should I try Karo next time? Anyone have a better method than the one I used for an LC? I'm eventually gonna do grain to grain but I heard it's pretty difficult to get grain sterilized without a pressure cooker. I'm just kind of using LC in the meantime.


--------------------
All said and done are not my words, all pictures are fictional, all questions are rhetorical. In fact this is an optical illusion.

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InvisibleJavadog
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Re: Need an Opinion on my LC pics [Re: zamora333]
    #15655482 - 01/12/12 02:03 PM (13 years, 8 days ago)

I have used honey and it is a fine nutrient.

Your problem is not the sugar you used, but an infected inoculant.

There is much discussion here about what is best.  I use
the standard LME+Dextrose, but any sugar will do in a pinch.

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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InvisibleTranscendingLife
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Re: Need an Opinion on my LC pics [Re: Javadog]
    #15655586 - 01/12/12 02:21 PM (13 years, 8 days ago)

The LC doesn't look right like, JD said.
Also, when taking a picture of an LC turn off all the lights in your room.
Then turn on one back light. That way you can see the mycelium growing.


Also, you can leave the Dextrose out of an LC recipe. It's a simple sugar & really adds nothing to the (E)LME.
(E)LME is a sugar made from grains &, IMHO, is the best for LCs.
You can pick up a pound for $5 at your local brewery or brew supply store online.
A pound lasts me over 2 years, too. Way cheaper than honey.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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InvisibleEvilMushroom666
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Re: Need an Opinion on my LC pics [Re: TranscendingLife]
    #15655621 - 01/12/12 02:29 PM (13 years, 8 days ago)

Quote:

TranscendingLife said:
The LC doesn't look right like, JD said.
Also, when taking a picture of an LC turn off all the lights in your room.
Then turn on one back light. That way you can see the mycelium growing.


Also, you can leave the Dextrose out of an LC recipe. It's a simple sugar & really adds nothing to the (E)LME.
(E)LME is a sugar made from grains &, IMHO, is the best for LCs.
You can pick up a pound for $5 at your local brewery or brew supply store online.
A pound lasts me over 2 years, too. Way cheaper than honey.



:whathesaid:

Once you make a few malt extract LC's you will never want to use anything
else.

Here is a LME LC (.6 gram added to 400ml of water) inoculated with a
few colonized rye kernels:

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InvisibleJavadog
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Re: Need an Opinion on my LC pics [Re: TranscendingLife]
    #15655690 - 01/12/12 02:41 PM (13 years, 8 days ago)

Quote:

TranscendingLife said:





Now, when I refer to "clear", this is what I mean.

Nice!

(:thumbup: on using kernels too)

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineShamrocker
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Re: Need an Opinion on my LC pics [Re: Javadog]
    #15655718 - 01/12/12 02:50 PM (13 years, 8 days ago)

As always, when using LCs, whip up a few BRF jars for testing purposes. It's the only way to verify a healthy LC. Good luck!

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InvisibleTranscendingLife
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Re: Need an Opinion on my LC pics [Re: Javadog]
    #15655720 - 01/12/12 02:50 PM (13 years, 8 days ago)

JD - that's a GLC that I hit up w/ 60cc of ELME water at 1g to 600mL.
I wanted to extend the life of it, so I gave the mycelium more food.
I normally inoculate my LCs w/ a GLC that way I know I have a clean culture.
I immediately use the GLC & store the LC for future use.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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Offlinezamora333
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Re: Need an Opinion on my LC pics [Re: TranscendingLife]
    #15670611 - 01/15/12 04:56 PM (13 years, 5 days ago)

Yeah I just figured out what I did wrong I pressure cooked the honey water for and hour and the honey carmalized :frown: that's why I got no growth gonna try again tonight except I'm gonna use that vanilla Karo instead


--------------------
All said and done are not my words, all pictures are fictional, all questions are rhetorical. In fact this is an optical illusion.

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Offlinekansastornado
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Re: Need an Opinion on my LC pics [Re: TranscendingLife]
    #15670829 - 01/15/12 07:13 PM (13 years, 5 days ago)

quote]TranscendingLife said:
The LC doesn't look right like, JD said.
Also, when taking a picture of an LC turn off all the lights in your room.
Then turn on one back light. That way you can see the mycelium growing.


Also, you can leave the Dextrose out of an LC recipe. It's a simple sugar & really adds nothing to the (E)LME.
(E)LME is a sugar made from grains &, IMHO, is the best for LCs.
You can pick up a pound for $5 at your local brewery or brew supply store online.
A pound lasts me over 2 years, too. Way cheaper than honey.





Does a mushroom tisue lc look like those? I have my first lc im going to use to see if it's good or not. It's very clear like those, but not as thick looking, it looks more like a huge dandy lion just floating around the mushroom tisue. I used just karo and water.

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InvisibleEvilMushroom666
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Re: Need an Opinion on my LC pics [Re: kansastornado]
    #15670839 - 01/15/12 07:18 PM (13 years, 5 days ago)

The thing about sugar based LC's is that you can never tell just by looking
at the culture if it is good or not.

Always inoculate a few small test jars, be it BRF or grain before moving
onto larger projects. A few drops on an agar plate works good as well.

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InvisibleTranscendingLife
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Re: Need an Opinion on my LC pics [Re: EvilMushroom666]
    #15671662 - 01/15/12 10:55 PM (13 years, 5 days ago)

Quote:

EvilMushroom666 said:
The thing about sugar based LC's is that you can never tell just by looking
at the culture if it is good or not.

Always inoculate a few small test jars, be it BRF or grain before moving
onto larger projects. A few drops on an agar plate works good as well.




QTF.
You'll find the sweet spot & hit it everytime w/ an LC.
Just takes some time.
I only use a GLC to inoculate an LC


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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OfflineBig_Sexy
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Re: Need an Opinion on my LC pics [Re: TranscendingLife]
    #15671747 - 01/15/12 11:17 PM (13 years, 5 days ago)

You need to only PC for about 15 minutes next time, and make sure you don't fill the jar so full because u need to be able to shake it. As far as clear or water looking LC goes it all depends on what u use, i use Malt syrup @3.5% and my LC's look like really dark piss and they always turn out good. I use the malt syrup because gretchen recommended it, she has been doing this for years and is the curator for the shroomery and she recommended malt syrup only after using everything else and finding malt syrup produces the most vigorous and thick growth, i concur after using dextrose and then malt powder. My LC's are ridiculous, just amazing, ill have to post pics some time but you guys wouldn't believe how much growth they have. And Ive never had one fully colonize in a week, more like 3 weeks or so.

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Offlinezamora333
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Re: Need an Opinion on my LC pics [Re: Big_Sexy]
    #15676630 - 01/17/12 03:27 AM (13 years, 3 days ago)

Yes please do :smile: What kind of malt syrup do you use?


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All said and done are not my words, all pictures are fictional, all questions are rhetorical. In fact this is an optical illusion.

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Offlinezamora333
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Re: Need an Opinion on my LC pics [Re: zamora333]
    #15709334 - 01/23/12 10:14 PM (12 years, 11 months ago)

Just started another one couple day back the ratio was perfect and I used agave nectar and getting nice growth. haaazzzaahh


--------------------
All said and done are not my words, all pictures are fictional, all questions are rhetorical. In fact this is an optical illusion.

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OfflineBig_Sexy
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Re: Need an Opinion on my LC pics [Re: zamora333]
    #15709362 - 01/23/12 10:22 PM (12 years, 11 months ago)

oops sorry i use barley malt syrup u can get it on amazon or a whole foods store. whats agave nectar?

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