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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Ultrasonic Humidifier

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Invisiblemobyd
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How do ya work with ya LFH???
    #1562347 - 05/20/03 06:08 AM (14 years, 7 days ago)

I want to know how you work with your Laminar Flow Hood?
I mean the whole process. From desinfect the table to transfer Agarpieces or inoculate jars.

I used the search function but I cant find anything about this......

I have always slightly contams on Agarplates. Could it be that I need more practise???

When you work, whats the distance (~) between Flow Hood and working area????


THX in advance,
mobyd


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OfflineAnnoA
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Re: How do ya work with ya LFH??? [Re: mobyd]
    #1562349 - 05/20/03 06:17 AM (14 years, 7 days ago)

>I have always slightly contams on Agarplates.
What kind?

>When you work, whats the distance (~) between Flow Hood and working area????

As little as possible, ie 10-20 cm.


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Invisiblemobyd
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Re: How do ya work with ya LFH??? [Re: Anno]
    #1562358 - 05/20/03 06:34 AM (14 years, 7 days ago)

>>I have always slightly contams on Agarplates.
>What kind?
Only mold because I use (most the time) Agar with penicillin.

Anno did you get my E-Mail???

The last time I worked with my LFH I used my Humidifier with a H2O2 Solution (let it run for about 20 mins). Maybe I can reduce contamns this way......

mobyd


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Invisiblemobyd
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Re: How do ya work with ya LFH??? [Re: mobyd]
    #1568009 - 05/22/03 02:29 AM (14 years, 5 days ago)

Anno ???

mobyd


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Edited by mobyd (05/22/03 02:32 AM)


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OfflineAnnoA
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Re: How do ya work with ya LFH??? [Re: mobyd]
    #1568115 - 05/22/03 03:01 AM (14 years, 5 days ago)

I don?t know...

It should work. Normally. Do you wash your hands thoroughly before doing work?
Did the filter get injured perhaps during assembly?


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Invisiblemobyd
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Re: How do ya work with ya LFH??? [Re: Anno]
    #1568316 - 05/22/03 06:06 AM (14 years, 5 days ago)

I always wash my hands with Hot water and soap. Then I spray Ethanol on them.

But I think I know now why I cant work sterile. Thats due to the frame of the filter. Its a wood frame and I always forgot to spray Alcohol on it. There was even a little dust on it!!! Hope that was my problem......
I?ll keep you updated.



mobyd


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OfflineJon_Doe
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Re: How do ya work with ya LFH??? [Re: mobyd]
    #1575378 - 05/24/03 11:56 PM (14 years, 2 days ago)

Anno, if you ever get cancer, i swear to god I'm gonna arrange a meeting with audrey for you

:smile: 


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OfflineAnnoA
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Re: How do ya work with ya LFH??? [Re: Jon_Doe]
    #1576262 - 05/25/03 01:53 PM (14 years, 1 day ago)

Now that?s a hard price to pay, but I?ll start smoking today.


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InvisiblePrisoner#1
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Re: How do ya work with ya LFH??? [Re: Anno]
    #1577858 - 05/26/03 07:56 AM (14 years, 1 day ago)

Mobyd...when you shut down your flow hood, wipe it down well, a little alcohol on a rag helps and it really doesn't harm the wood....cover it with a heavy guage plastic and apply a little tape as may be needed.....I have one like the TLG flow hood (10 of them originaly) it's simply a matter of cleaning it up and flipping it face down on a clean surface...


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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood, Ultrasonic Humidifier

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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