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OfflineJekyll
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Registered: 08/06/11
Posts: 35
Last seen: 12 years, 2 months
How can I grow a fungi from a dried sample?
    #15613938 - 01/04/12 06:05 AM (13 years, 15 days ago)

So I've been trying to find ways at reviving tissue, cloning, or regrowing fungi from dormant spores and I'm wondering how I would do this or start? Any tips? I am unable to find other teks about this.

I want to to grow some fungi from a dried, possibiliy contaminted piece of tissue. I am unable to order agar, but I guess I can make some or use some sort of karo extract. I have no idea how to do this however because I'm a newb. Can anyone please help?.

Is there some sort of step by step instruction? Would someone be so kind as to write one up?

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OfflineNewbusMaximus
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Registered: 07/11/11
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15613961 - 01/04/12 06:25 AM (13 years, 15 days ago)

i did some research to suggest its not a very  promising venture, trying to clone from dried fruit. but if your dried fruits dropped spores you might could retrieve some by squirting sterile water between the gills and suckin back up a few times. never tried it but if it works you could make your own tek... gl

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Offlinetrophycase
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Registered: 03/23/11
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15613962 - 01/04/12 06:25 AM (13 years, 15 days ago)

Quote:

Jekyll said:
So I've been trying to find ways at reviving tissue, cloning, or regrowing fungi from dormant spores and I'm wondering how I would do this or start? Any tips? I am unable to find other teks about this.



You must not have been looking very hard.
Quote:

RogerRabbit said:
Here's a process that has been 100% successful in reviving dry mushroom tissue for me so far. Don't use peroxide, whatever you do. It's toxic to fungi cells, and the last thing you want to do when reviving tissue is harm it further.

Sterilize several jars of distilled water, one of which should be prepared with small air exchange holes in the lid and a method of filtering, such as tyvek or synthetic filter disk, polyfill, etc.

Break up the dry tissue into grain sized chunks and put a dozen or so into one of your sterilized jars of water. Shake well to wash the pieces of tissue, and then transfer them to the next jar of sterile water. (you can pick up the pieces with a syringe needle or scalpel) Shake again to wash and repeat.

When you place them into the final jar, which is filtered, swirl them around in the water and then set the jar away and forget about it for a few weeks. You'll begin to see mycelium growing on any chunks that are viable. Don't use nutrients of any kind, just plain sterile distilled water. Once mycelium begins to form, transfer to agar to isolate away from any contaminants that may be present.
RR




Taken from this thread: http://www.shroomery.org/forums/showflat.php/Number/8478760#Post8478760

Took about a minute to find. Do us all a favor next time and do some reading before you start asking questions please.


--------------------
All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.

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OfflinepsyShroomer
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15613965 - 01/04/12 06:27 AM (13 years, 15 days ago)

Fist off you are asking 2 different questions.  If you are trying to revive dormant spores, that's easy.  You need to hydrate, then allow them to germinate. 

deleted I was mistaken.


--------------------
"Better to remain silent and be thought a fool than to speak out and remove all doubt."
-Abraham Lincoln

Edited by psyShroomer (01/04/12 06:28 AM)

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OfflineNewbusMaximus
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Re: How can I grow a fungi from a dried sample? [Re: psyShroomer]
    #15613971 - 01/04/12 06:30 AM (13 years, 15 days ago)

that sounds way better than my idea.

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OfflineJekyll
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Re: How can I grow a fungi from a dried sample? [Re: trophycase]
    #15615011 - 01/04/12 12:43 PM (13 years, 15 days ago)

Quote:

stoned2676 said:
Fist off you are asking 2 different questions.  If you are trying to revive dormant spores, that's easy.  You need to hydrate, then allow them to germinate. 

deleted I was mistaken.




What's the other question, and answer?


Quote:


Took about a minute to find. Do us all a favor next time and do some reading before you start asking questions please.




I actually read that post before but thanks. :]

I said I was unable to use agar in the OP. What can I use instead of agar? I no access to a flow hood or a glove box, I am thinking doing agar in my environment is not sterile enough.

Either way, wouldn't take a flow hood and an extremely sterile environment for this to work?

If I HAVE to buy agar, can regular agar I'd buy at a retail store/science shop suffice?

After you get mycelium tissue growing successfuly, I'm assuming you transfer them to jars?

Edited by Jekyll (01/04/12 01:17 PM)

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Offlinetrophycase
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15615071 - 01/04/12 01:01 PM (13 years, 15 days ago)

Your sample is almost 100% guaranteed to be contaminated. I don't know how you would get a clean sample without agar...


--------------------
All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.

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OfflineJekyll
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Re: How can I grow a fungi from a dried sample? [Re: trophycase]
    #15615179 - 01/04/12 01:28 PM (13 years, 15 days ago)

Quote:

trophycase said:
Your sample is almost 100% guaranteed to be contaminated. I don't know how you would get a clean sample without agar...




can I use something from here?

http://www.shroomery.org/9393/FastFreds-Media-Cookbook

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Offlinebishlap
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Re: How can I grow a fungi from a dried sample? [Re: trophycase]
    #15615349 - 01/04/12 02:09 PM (13 years, 15 days ago)

I actually tried something like this a few months back.
although I was expecting failure due to my frugality, to purchace a clean sample.
what I did was take a few 1/8 - 1/4" sized pieces just a small handful, and "in clean chamber" I added them to a jar of pre sterilized water I made for the experiment allowed to sit for 24 hours then shook the hell out of it and let it sit for 24 more hours.
when I came back to it everything I thew in was still floating but there was a fine layer of blue/green on the bottom.
then I sterilized everything put it in the box poured out about half removing the chunks.
then shook it again to spread spores, filled 2  10cc syringes.
then i filled 2 dozen old petri dishes with sterilized rye grain, and I would strongly suggest you don't do this condensation forms and drips causing wet/dry spots.
but I was expecting failure so I went for numbers.
after inoculations it took 3-5 days and nearly all oc them showed some growth but shortly thereafter I noticed a grey fuzz almost looked like wet ashes I removed all that showed signs of contamination untill I finally was down to 3, as a last dich effort I tossed a small piece 2 grains grown together into a fresh jar and Viola.

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OfflinepsyShroomer
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15615676 - 01/04/12 03:27 PM (13 years, 15 days ago)

This reads as if you are inquiring about three distinct teks.  But since cloning and reviving would produce a similar outcome. (plus I didn't think reviving to be possible) I assumed 2 questions. 

Quote:

Jekyll said:
So I've been trying to find ways at reviving tissue, cloning, or regrowing fungi from dormant spores and I'm wondering how I would do this or start? Any tips? I am unable to find other teks about this.




"Regrowing from dormant spores" actually it would just be growing.  Since regrowing implies previous growth, and germinating from spores, dormant or otherwise, would produce a new mushroom.  (that's analogous to writing "regrowing a chicken from an egg.)


Quote:

Jekyll said:
I want to to grow some fungi from a dried, possibiliy contaminted piece of tissue. I am unable to order agar, but I guess I can make some or use some sort of karo extract. I have no idea how to do this however because I'm a newb. Can anyone please help?.





Then reviving "from dried possibly contaminated tissue".  I didn't think that was possible since dehydration usually means death to the living tissue, but I was mistaken.  TrophyCase supplied the answer to that question.

But I'm glad to see it is possible.  Good luck to you and I hope I clarified to your satisfaction. 

Also, I'm not sure from whom I read this, but someone made a great suggestion once of using a trash bag for a still air system in a pinch.  If I run across it again I will be sure to give credit.


--------------------
"Better to remain silent and be thought a fool than to speak out and remove all doubt."
-Abraham Lincoln

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OfflineJekyll
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Re: How can I grow a fungi from a dried sample? [Re: bishlap]
    #15676607 - 01/17/12 03:12 AM (13 years, 2 days ago)

Quote:

bishlap said:
I actually tried something like this a few months back.
although I was expecting failure due to my frugality, to purchace a clean sample.
what I did was take a few 1/8 - 1/4" sized pieces just a small handful, and "in clean chamber" I added them to a jar of pre sterilized water I made for the experiment allowed to sit for 24 hours then shook the hell out of it and let it sit for 24 more hours.
when I came back to it everything I thew in was still floating but there was a fine layer of blue/green on the bottom.
then I sterilized everything put it in the box poured out about half removing the chunks.
then shook it again to spread spores, filled 2  10cc syringes.
then i filled 2 dozen old petri dishes with sterilized rye grain, and I would strongly suggest you don't do this condensation forms and drips causing wet/dry spots.
but I was expecting failure so I went for numbers.
after inoculations it took 3-5 days and nearly all oc them showed some growth but shortly thereafter I noticed a grey fuzz almost looked like wet ashes I removed all that showed signs of contamination untill I finally was down to 3, as a last dich effort I tossed a small piece 2 grains grown together into a fresh jar and Viola.




Oh wow this is a good idea, thanks.

So instead of agar you used rye grain..hmm...

Perhaps I could use some other food source like that..other than rye grain because you said it causes wet/dry spots.

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Offlinefraggy
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Re: How can I grow a fungi from a dried sample? [Re: Jekyll]
    #15755682 - 02/03/12 12:01 PM (12 years, 11 months ago)

Just very recently I found out about the cardboard tek. I immediately fell in love with it. Never tried it but I will, since my first experience with agar went sour (my own fault, no doubts about it, but I'm a Noob too).

Then I read that cardboard is great for wood loving species and it's especially great for us noobs :smile: since it is less prone to contaminants and a lot easier than agar. And cardboard can be found everywhere :smile:

I think you'd better use cardboard instead of agar!!! RR, I would love to hear your opinion about that.

Note: regular agar will do (although you will need to add nutrients to it) and can be bought at your local health food shop :smile:

Cardboard on the other hand... Is very widely available... And when shredded can even be used for casing according to another thread I read on this forum.

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OfflineKizzle
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Re: How can I grow a fungi from a dried sample? [Re: fraggy]
    #15755930 - 02/03/12 01:03 PM (12 years, 11 months ago)

Quote:

Then I read that cardboard is great for wood loving species and it's especially great for us noobs :smile: since it is less prone to contaminants



Being resistant to contaminants isn't necessarily a good thing though. If your culture is contaminated you want to know before you inoculate something with it. You might have contaminant spores that won't germinate on the cardboard making it look clean, then transfer it to a jar only to find them germinating on the grains.


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Offlinebishlap
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Re: How can I grow a fungi from a dried sample? [Re: Kizzle]
    #15756171 - 02/03/12 02:06 PM (12 years, 11 months ago)

I saw a thread somewhere on this site can't find it now.
where a member was collecting stems spawning them to cardboard and fruiting them it was very interesting.


--------------------
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to much, you didn't take enough" -
Terrence McKenna

There is no soul, only the ego dies.
The body was never yours.

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Offlinefraggy
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Re: How can I grow a fungi from a dried sample? [Re: bishlap]
    #15756261 - 02/03/12 02:42 PM (12 years, 11 months ago)

True, but then you'd already have a quite dominant growth of your chosen species, or at least that one species having a very big headstart over the possible contaminants.

I've seen teks beeing done without even cooking the cardboard and people use casings of shredded cardboard as medium. So not even necessary to work sterily... But if you stick to sterile working, even better!

I've gotta see it for myself yet though... Anyway, the cardboard being a semi selective medium definitely offers an advantage that other mediums don't and that's why those shroom hunters like to use it. I suppose it will be better if I start a dedicated thread about that instead of cross linking from here.

Edited by fraggy (02/03/12 02:46 PM)

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