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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Vermiculite

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InvisibleLucidDream
Hungry BlueFiend
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Registered: 05/09/03
Posts: 1,488
Loc: Planet of the Stupid Peop...
What Went Wrong: an analysis
    #1561355 - 05/19/03 10:03 PM (13 years, 6 months ago)

The standard PF Tek was followed to a T. Well, almost. Each batch of jars out of the pot was left to cool on a table so another could be boiled in a hurry. I wonder if I blew it right there. The three groups of jars had 7, 5 and 3 hours respectively to cool down.

After sterilization, the 12 1/2 pint jars were innoculated with 10cc GT.
They spent 4 days in a very dark, dry and disinfected place at 72 degrees. On the 5th day, concerned about the lack of development, they were placed in an inch of water heated to between 80 and 85 degrees, where they remained for the next week. No signs of life.

On the positive side, the jars certainly seem to have been sterilized (!), because absolutely nothing grew in there. Even now, after 13 days, no signs of life. I've thought about injecting a little sterile water for shits and giggles, to see if I can tease anything out of them.

Another possible glitch that may have contributed to the problem: the verm layer on top is actually an inch thick rather than a half-inch. I guess these little details are important. Also, the boiling water probably got up too high during sterilization. It may have gotten up into the foil. Next time gotta keep it lower and smoother.

Haste makes waste, they say...

Tonight's adaptation: 10cc of Malabar from the frige. I'm thinking, don't blow it, man. But the little bastard spores are cold and clumped together. They won't separate. This time I've let the jars cool overnight in a new big pot, and there are only six of them this time. Also they have five holes instead of four. The water was about an inch and a half deep around the jars atop 2 small towels. Gentle rolling boil for 90 minutes. Foil out of the water at all times.

Shot the first jar and was so intent on counting cc's I didn't notice that most of those visible spore clumps were gone until I went to the second jar. Fuck! Gotta keep your eye on the ball! So the first jar got probly 90% of the ss. I tell you, it's a comedy of errors with me. So what the fuck, let's just pump lots of this shit into the rest of them, right? I mean it's worthless anyway at this point. Two of them really got extra. Next, gotta clean the incubator and set'em up again. It would all be funny if it weren't so sad...


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Sarcasm just one of my many talents.



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InvisibleLe_Canard
Danger Man

Registered: 05/17/03
Posts: 93,269
Loc: Earthfarm 1
Re: What Went Wrong: an analysis [Re: LucidDream]
    #1561722 - 05/19/03 11:50 PM (13 years, 6 months ago)

*A thinly veiled "BUMP"*
/me eagerly awaits an analysis from the local experts!  :smile: 


Edited by Le_Canard (05/19/03 11:51 PM)


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OfflineRustik
Where am I?

Registered: 04/18/03
Posts: 289
Last seen: 2 years, 22 days
Re: What Went Wrong: an analysis [Re: Le_Canard]
    #1561746 - 05/19/03 11:58 PM (13 years, 6 months ago)

Were the jars cool to the touch when you innoculated?

If they weren't cool, there's a possibility the spores could have been killed by the heat.

How long are the needles you're using?

If they aren't reaching below the verm layer, you might be releasing the spores onto the non-nutritious verm. They won't germinate there.

Who did you purchase the spores from?

Open one of the jars, and check for contamination (waft your hand over the top of the jar and take a small sniff). If it smells at all funky, it's probably a bacterial contam. If there is no smell, and you are sure they aren't contaminated, then contact the vendor and see if you can't get a replacement.


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The blue... the blue!!!


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InvisibleLucidDream
Hungry BlueFiend
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Registered: 05/09/03
Posts: 1,488
Loc: Planet of the Stupid Peop...
Re: What Went Wrong: an analysis [Re: Rustik]
    #1561765 - 05/20/03 12:04 AM (13 years, 6 months ago)

Jars cool: yes, to the touch. Needle was easily long enough to reach the substrate and touch the glass.

"Who did you purchase the spores from?"

Hmmm...I won't mention. They are blameless. The spores were good. They were real. It was my fault somehow.

Thanks :smirk: 


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Sarcasm just one of my many talents.



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OfflineArtifice
Poet

Registered: 03/09/03
Posts: 57
Last seen: 13 years, 2 months
Re: What Went Wrong: an analysis [Re: LucidDream]
    #1562037 - 05/20/03 02:00 AM (13 years, 6 months ago)

I would wait 1 to 2 days for the jars to cool. If they were sterilized nothing will grow in them. I ve waited a whole month after sterilizing jars before innoculating. Of course this was not on purpose, but the jar colonized quikcer then the others, and i didnt have to worry about the heat killing the spore, or worrying about possible infection form unsteralized substrate because contamination would have grown by then. 2 to 3 hours was probabaly not a long enough wait, even if the jar was cool to the touch, you still cant touch the inside and feel the subustrate. It is also possible that contamination was there and germinated before the mush spores. I have had containmations that do not show up for several weeks. One is this slow growing black thing, nasty looking. It didnt show up for a month, and was in the same spot my inncoulation site was, so im asssuming that this black thing prevented the spores form germanating.


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,121
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3 in 1 [Re: LucidDream]
    #1562083 - 05/20/03 02:30 AM (13 years, 6 months ago)

innoculation:
it?s not.
It?s inoculation with 1 N.

No more, no less.
1 N at a time.
inoculation.
inoculate.


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OfflineWapo
journeyman
Registered: 05/04/03
Posts: 57
Last seen: 11 years, 7 months
Re: 3 in 1 [Re: Anno]
    #1562375 - 05/20/03 07:03 AM (13 years, 6 months ago)

nice pics anno i take they are of you


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
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Re: 3 in 1 [Re: Wapo]
    #1562377 - 05/20/03 07:04 AM (13 years, 6 months ago)

You shouldn?t draw conclusions so fast.


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OfflineDank420
Shrooms...Mmm

Registered: 04/12/03
Posts: 548
Loc: 'burbs of philly
Last seen: 8 years, 8 months
Re: 3 in 1 [Re: Wapo]
    #1562421 - 05/20/03 08:31 AM (13 years, 6 months ago)

LOL Wapo: "The actress in my avatar is Audrey Tautou." -Anno


--------------------
"I tried marijuana once. I did not inhale." -William Clinton.


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Invisibletak_old
Endo Smoke

Registered: 05/31/02
Posts: 609
Loc: State of confusion
Re: 3 in 1 [Re: Dank420]
    #1562633 - 05/20/03 12:10 PM (13 years, 6 months ago)

That happend to some Pes Amazonians I tried. They sat and sat, no contams as of yesterday (4 weeks). No signs of growth either. We tossed them.


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InvisibleLe_Canard
Danger Man

Registered: 05/17/03
Posts: 93,269
Loc: Earthfarm 1
Re: 3 in 1 [Re: tak_old]
    #1563742 - 05/20/03 08:24 PM (13 years, 6 months ago)

Oh well... :frown: Thanx for the input everyone! Its much appreciated. As LucidDream and I are working on this together (He more so than I) , we're *quite* the newbies to "recreational mycology" (A-HEM!  :grin:). We live and learn, I suppose.. *sigh* Anyhoo, thanx again, folks! 


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Vermiculite

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