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OfflinePDU
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Cloning mycelium culture in honey?
    #1560778 - 05/19/03 06:54 PM (13 years, 6 months ago)

Its not exactly cloning...but, i have a honey culture going, started with maz spores, however, knowing that peroxide kills spores i did not add any. This afternoon I pc'd 3 more half pints with honey which i was planning on injecting with mycelium solution from the original culture..
My question is; 2cc's of peroxide in each of the new jars would be fine correct? As it will technically be adding a tissue sample...


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OfflineFocusHawaii
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Re: Cloning mycelium culture in honey? [Re: PDU]
    #1560814 - 05/19/03 07:06 PM (13 years, 6 months ago)

Hard to say, I would think you could because there are peroxidated Agar plates, try it with one and get back to us :smile:.


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Offlinelemunhed
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Re: Cloning mycelium culture in honey? [Re: FocusHawaii]
    #1561617 - 05/19/03 11:12 PM (13 years, 6 months ago)

If you use filter disks and PC it enough, or microwave it or whatever you do, then don't worry too much about peroxide. I would wager that it's pathogenic to a developing mycelium to add alot of peroxide to the solution. Its a different story when your using it in direct contact with the mycelium, and in relatively large quantities. When its in the bottom of your tank or beign sprayed on msuhies, its not in the mass amounts you would want in a honey culture ... i bet 2cc would be ok but i dont know how much that would do for your anti bacterial action. Honey itself is supposed to be mildly antibacterial. Try it out though and make sure to post your results!


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OfflinePDU
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Re: Cloning mycelium culture in honey? [Re: lemunhed]
    #1562024 - 05/20/03 01:47 AM (13 years, 6 months ago)

Well, ive read in a couple teks about adding peroxide...i use bandaids over the holes...suposedly its beneficial because when peroxide breaks down it makes oxygen. Anyways...ill try. Hope it works


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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