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Offlineredfoot
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Registered: 05/07/03
Posts: 96
Last seen: 10 years, 1 month
Coffee Filter Question
    #1559924 - 05/19/03 02:01 PM (13 years, 6 months ago)

AFOAF has 7 jars in an incubator. On top of the jars he has coffee filters cut to cover the top of the jars and cover his holes. From what i have read this is unnessecary because the top of the incubator is enclosed and there is clean air exchange in there.

Question: Would removing the coffee filters increase colonization of the jars?


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Invisibleafoaf
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Registered: 11/08/02
Posts: 32,665
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Re: Coffee Filter Question [Re: redfoot]
    #1559937 - 05/19/03 02:07 PM (13 years, 6 months ago)

coffee filters are a good additional barrier
to help filter out the larger nasties.

removing them won't make much difference
as air transfers easily through them.

this one dude I know puts 2-3 coffee filters
rubber banded over all his quarts, which already
have a small tyvek filter built into the lids.

this serves two purposes:

1. redundant filtration for larger particles
2. creates a good temporary surface to write on
(noting strain, innoc date, transfer date and the like)

bottom line, the lid on your incubator won't keep dirties
out when you open it up, and if dirties do get in, there isn't
any sort of clean air exchange in your incubator unless you've
fitted it with a fan and additional filter...so, again, some extra
filtration for your jars couldn't hurt.


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Offlinedebianlinux
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Registered: 12/09/02
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Re: Coffee Filter Question [Re: redfoot]
    #1559941 - 05/19/03 02:08 PM (13 years, 6 months ago)

i assume you are referring to PF-style jars?...
Coffee filters will do little to impede gas exchange and therefore should show no effect on colonisation times.


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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 5 years, 8 months
Re: Coffee Filter Question [Re: debianlinux]
    #1560926 - 05/19/03 07:44 PM (13 years, 6 months ago)

lol! My foaf writes on his coffee filters too!


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OfflineEkstaza
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Re: Coffee Filter Question [Re: Demiurge]
    #1561303 - 05/19/03 09:44 PM (13 years, 6 months ago)

My FOAF, TOO.


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InvisibleMagashM
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Re: Coffee Filter Question [Re: redfoot]
    #1561653 - 05/19/03 11:25 PM (13 years, 6 months ago)

Put a layer of cardboard between layers of jars when there colonizing. This may sound stupid but it doesn?t make sense to take off the tape then stack them in a way that blocks the holes. This was when I was still leaving tape on and making all the classic mistakes. Took up to 4 weeks for jars to finish.



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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Magash]
    #1561662 - 05/19/03 11:26 PM (13 years, 6 months ago)

Post deleted by administrator


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Coffee Filter Question [Re: redfoot]
    #1561679 - 05/19/03 11:33 PM (13 years, 6 months ago)


Big lateral filing cabinets make good incubators. A simple small ceramic heater with a themostat on the bottom level maintains the temp, just fine.

The coffee filters are just dust covers & do not impede gas exchange.

6T............. :wink: 


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Invisibleafoaf
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Re: Coffee Filter Question [Re: Magash]
    #1561692 - 05/19/03 11:37 PM (13 years, 6 months ago)

genius!

do you use that zip tie to keep the bin low
in the water even when there's not enough
weight in it to keep it submerged?

afoaf's temps fluctuate depending on the load
in the bin (thermogenisis is also a factor, I'm sure).


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