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Registered: 05/10/03
Posts: 49
Loc: West Coast
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re-inoculation question
    #1559059 - 05/19/03 01:16 AM (14 years, 13 days ago)

On thursday morning AFOAF inoculated 6 jars with 1 syringe of Lipa Yai, pretty evenly distributed among the jars. The jars have had constant 70-80 degree warmth, and show no signs of colonization. Should he inoculate them with his second syringe of Lipa Yai, as he's been urged to do, or should he wait a few more days?

Not that we needed all that for the trip, but once you get locked in a serious drug collection, the tendency is to push it as far as you can...

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Registered: 03/31/03
Posts: 211
Loc: US
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Re: re-inoculation question [Re: ChemicalPixi]
    #1559084 - 05/19/03 01:31 AM (14 years, 13 days ago)

Some of my jars didnt colonize until the 12th, and they were inoculated on the 1st

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bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
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Re: re-inoculation question [Re: photovorix]
    #1559095 - 05/19/03 01:37 AM (14 years, 13 days ago)

Wait another 2 weeks; it could take that long.


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stranger thanmost
Registered: 04/30/03
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Re: re-inoculation question [Re: micro]
    #1559147 - 05/19/03 02:12 AM (14 years, 13 days ago)

yes wait, and try to keep temp closer to 80 than 70 , found 82 to be ideal formy friend

u know it aint funny when u aint got no money and the clock strikes 420 and u wanna get high
~Kottonmouth Kings

Edited by MykeOfile (05/19/03 02:12 AM)

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Registered: 04/25/03
Posts: 430
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Re: re-inoculation question [Re: MykeOfile]
    #1559220 - 05/19/03 03:02 AM (14 years, 13 days ago)

Wait a little longer, and keep them at 80-85 degrees. You can re-inoculate, I have done so. However, I later found out that it was not necessary.


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Re: re-inoculation question [Re: ChemicalPixi]
    #1559276 - 05/19/03 03:39 AM (14 years, 13 days ago)

IMHO, knocking up the same jar, after days and/or weeks have past. Is like throwing good money down a bad hole. Why do that? Prep fhresh jars & knock those up. Old jars have a higher chance of a contam. Sort of like using the same rubber -- twice..... :smirk: -- not a good idead :grin:.

If I were you, I would wait & see what the existing jars do. You could also prep some more & knock those up too, if you have the urge. Oh ya.......... 70 to 80 F is not constant. That is a 10 degree fluctuation (6T counts on his fingers). 82 to 84 F contstant will do you better.

6T  :wink: 

~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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