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Invisiblemicro
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Spectrophotometer readings
    #1558703 - 05/18/03 10:40 PM (14 years, 4 months ago)

Anyone know absorbance wavelengths for psilocybin/psilocin?

Thanks,

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Micro


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Invisiblewhiterasta
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Re: Spectrophotometer readings [Re: micro]
    #1561104 - 05/19/03 08:42 PM (14 years, 4 months ago)

I worked on using a spectrophotometer as a method for doing production assays of diazo coupling compounds.The compounds I was working were proprietary and little "formal" data was available(such as absorbsion wavelength).So I had to determine aborbsion wavelengths through sampling at various wavelength ultra purified samples until distinct "holes" appeared in graphing the results.
Please remember this was 20+ yrs ago and I was working with used and shoddy equipment but I did achieve a reliable "field assay" for the production area.
In assaying for tryptamines,unless this data exists in some database some where.You will possibly need to prepare an ultrapure extract and determine the wavelength through similar experimentation.
I first ran a series at 100nm separation and this was inconclusive so I reran at 10nm separation and the two distinct spikes emerged,a smaller spike and  a much larger that was the wavelength I used (The damn numbers escape me right now)
Impurities deviated the clean numbers by often a factor of 100nm so a solid benchmark number is vital.
If you find out where to get the absorbsion wavelngth or you determine it PLEASE post your results,I would greatly appreciate it.I hope I was of some help  :tongue: WR


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OfflineRaadt
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Re: Spectrophotometer readings [Re: micro]
    #1561134 - 05/19/03 08:57 PM (14 years, 4 months ago)

You might consider emailing andrew shulgin.

He would probably have an answer, as he's bioassayed a few mushrooms it seems :wink:


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Invisiblewhiterasta
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Re: Spectrophotometer readings [Re: Raadt]
    #1561316 - 05/19/03 09:49 PM (14 years, 4 months ago)

:grin: I thought that also but Sasha is getting older and doesn't keep up on his E-mail very well any more.Perhaps a question posted at Alchemind would get through the best.Good Luck............and DO let us know what you find if you would.WR


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OfflineElektrolurch
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Re: Spectrophotometer readings [Re: whiterasta]
    #1564047 - 05/20/03 10:34 PM (14 years, 4 months ago)

Hi,

In the Book "Le Champignong Hallucinogene du Mexique" (Heim & Cailleux) there is a Chapter written by Hoffman about the synthesis of Psilocybin/Psilocin. In that chapter you'll find the spectra. I don't have access to my copy, therefor I can't scan it for you.

mjshroomer has one though. He could scan them for you, if you ask him. Look for his post "Roger Heim's Cultivation of P. semperviva (French with Pics)", this are actually scans from the book I'm talking about...

Elektrolurch


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The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67


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InvisiblePinback
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Re: Spectrophotometer readings [Re: micro]
    #1565381 - 05/21/03 12:09 PM (14 years, 4 months ago)



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OfflineAnnoA
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Re: Spectrophotometer readings [Re: micro]
    #1565391 - 05/21/03 12:15 PM (14 years, 4 months ago)



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InvisibleJoshua
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Re: Spectrophotometer readings [Re: Anno]
    #1566624 - 05/21/03 06:44 PM (14 years, 4 months ago)

The two spectra are for natural (left) and synthetic (right). I am curious as to why there is any difference. They apear identical except for the baseline. Is it possible they did not calibrate the machine properly for one of the readings?

Joshua


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OfflineElektrolurch
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Re: Spectrophotometer readings [Re: Joshua]
    #1566990 - 05/21/03 09:06 PM (14 years, 4 months ago)

Hi,

they are not identical, there are some slight differences (e.g. ca. 900 cm?1), beside the "baseline" effect. This differences are because an extract will always contain small amounts of other compounds. This is also true for the synthetic compound. The difference is that for the synthetic compound you know which impurities are contained, while for the natural one you not necesarly do.

The "baseline" effect is because the absorbtion, and thus the transmission too, depends on the concentration of the solution. This is known as the "Lambert-Beer" law. The probes measured had probably two slightly different concentrations, and hence the baseline is slightly different. Actually the form of the curve is important for the identification.

Elektrolurch


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"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67


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InvisibleJoshua
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Re: Spectrophotometer readings [Re: Elektrolurch]
    #1567337 - 05/21/03 10:45 PM (14 years, 4 months ago)

Thank you for the good explaination.

I took another look and it appears the lowest valley of both spectrums are set to equal values. Impurities, related compounds as well as a difference in concentrations seems like a very good explaination.

Joshua


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"Life After Death is Saprophytic!"


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Invisiblemicro
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Re: Spectrophotometer readings [Re: Joshua]
    #1570131 - 05/22/03 08:40 PM (14 years, 4 months ago)

Thanks, people! Anno + pinback, esp.

I've been AFK, so haven't posted for a few days.

My spectrphotometer is really old, but I'm sure I can at least get readings with the proper lambda, so thanks! I'll post results once I can but I haven't even calibrated the old piece of junk, yet. I really only want it for approximate conc's in solutions, though; I may not use it until my phalaris grass experiments....

Thanks again,

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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