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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
this guy is killing my rotation....
    #1557608 - 05/18/03 12:33 PM (13 years, 11 months ago)

trich, aspergillus, other?

any ID help would be greatly appreciated.

it's killing bins during colonization and even
those that have made it to 100% and into
the chamber seem to succumb only days
later.

I can't tell if it's cross contamination due to
insufficient cleaning between uses (bins),
bad pasteurization temps and/or tainted shit.









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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: this guy is killing my rotation.... [Re: shroomQT]
    #1557889 - 05/18/03 04:16 PM (13 years, 11 months ago)

What's the humidity around the casings? Also -- my monitor sucks, but I think I see pieces of uncolonized straw sticking out all over the place -- if this is the case that's probably why it's getting contaminated.

--
Micro


--------------------
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(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: this guy is killing my rotation.... [Re: micro]
    #1558054 - 05/18/03 05:34 PM (13 years, 11 months ago)

the humidity is really just the foggy plastic container
that they casing colonizes in.

there is no excessive moisture during colonization.

the casing looks that way because it got thrown
across the garage when I saw that it was contaminated.

I am very upset, this particular foe seems to be
quite formidable...

while there are spots where the straw is exposed, that
does not seem to be the root of the contamination as it
occurs in both bare and previously colonized spots.


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 3 months, 23 days
Re: this guy is killing my rotation.... [Re: shroomQT]
    #1558144 - 05/18/03 06:28 PM (13 years, 11 months ago)

Have you tried soaking your bins/terrerium (lids as well) in hot water with a few cups of bleach added after dumping a contaminated casing?


--------------------
Just another spore in the wind.


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: this guy is killing my rotation.... [Re: Seuss]
    #1558179 - 05/18/03 06:54 PM (13 years, 11 months ago)

soaked, no.

but they are scrubbed with antibacterial
dish washing soap and a splash of straight
bleach.

is the contaminant familiar to anyone?

it grows a fuzzy white and eventually
turns a light green, growing darker with
time.

it moves quite quickly...one day it's just
there.


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: this guy is killing my rotation.... [Re: shroomQT]
    #1558222 - 05/18/03 07:20 PM (13 years, 11 months ago)

trichoderma (trich.)

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleshroomQT
psilly gurl

Registered: 05/05/03
Posts: 249
Re: this guy is killing my rotation.... [Re: micro]
    #1558259 - 05/18/03 07:48 PM (13 years, 11 months ago)

any way to kill it and not the mycellium?

or is it a lost cause...?


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: this guy is killing my rotation.... [Re: shroomQT]
    #1558306 - 05/18/03 08:16 PM (13 years, 11 months ago)

salt. See Hippie's post on the top of the list in this forum.

Better to catch it early on.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineSeussA
Error: divide byzero

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Registered: 04/27/01
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Re: this guy is killing my rotation.... [Re: shroomQT]
    #1560439 - 05/19/03 05:02 PM (13 years, 11 months ago)

Quote:

soaked, no.

but they are scrubbed with antibacterial
dish washing soap and a splash of straight
bleach.




I think you have a spore generating contamination inside your terrarium. Spores are hardy little bastards that are hard to kill or get rid of. Antibacterial soap will do nothing for a fungal contamination, nor will it kill many bacteria. Almost all common household bacteria are now immune to antibacterial soaps.

Before your next rotation try soaking everything that has been inside, or will go inside, the terrerium in hot water with a cup or two of bleach added. You need to let it soak for several hours and then rinse well. Be sure you get the terreriums, the lids, any hoses or tubing, the casing trays, etc. Let everything dry and then wipe it down with a 70% (not 100%) solution of IPA (rubbing alcohol). If you have a humidifier hooked up to the system, was it down as well... If you have a UV-C lamp, let everything bake for a few hours under it... (normal black lights are UV-B and do not work the same... UV-C lamps are what they use in fast food chains, supermarkets, hospitals, etc).

You keep seeing the same thing come back, so you know it is somewhere hiding. Once you see it, go back 24-48 hours and think about what happened during that time frame. It is pointless to try and fight it until you figure out where it is coming from in the first place.


--------------------
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Invisiblemicro
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Re: this guy is killing my rotation.... [Re: Seuss]
    #1571243 - 05/23/03 03:28 AM (13 years, 11 months ago)

Trichoderma is airborne; not just a surface contaminant. I doubt that a significant amount will stay on the container, but that's just IMO. Trich needs something to hold on to (food that it can digest) and can't just live off of pure (meaning ideal) casing material. It seems that way, though, because it sporulates mad early and gets spores everywhere which germinate, grow and cause a serious problem.

My roomate has 2 casings covered in Trich + cobweb right above and next to a straw casing of mine. If the problem is "contamination" his contaminants would be sporulating all over my stuff and contaminate it, but my stuff is fruiting fine where it is with no contamination, at all.

Sterility will certainly help in preventing trich (unless you use peat -- it is usually in the peat) but care must be taken so that nothing undecomposed, plant-related and uncolonized is in or near the casing. The Trichoderma will attack it and sporulate all over the place. Also avoid stuff like unpatched overlay which seems to affect the mycelium in that area in a negative way, leaving it succeptible to contamination.

Just my 2c

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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