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Offlinethe_hatter
half mad

Registered: 04/18/10
Posts: 753
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glc knotting or contamination?
    #15538042 - 12/19/11 01:19 AM (12 years, 2 months ago)

is this what i think it is?  if it is i cant wait to use this!



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Offlinekeeno
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Registered: 06/01/11
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Re: glc knotting or contamination? [Re: the_hatter]
    #15538187 - 12/19/11 03:24 AM (12 years, 2 months ago)

looks like jizz in a jar

:awedance:

haha, really can't tell from the pics.
but wanted to wish you luck there!


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InvisibleStropharis
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Re: glc knotting or contamination? [Re: keeno]
    #15538220 - 12/19/11 03:41 AM (12 years, 2 months ago)

Yea, looks kind of like this



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InvisibleStropharis
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Re: glc knotting or contamination? [Re: Stropharis]
    #15538222 - 12/19/11 03:42 AM (12 years, 2 months ago)

Just fucking with you man, you should try and get some better photos.


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OfflineKizzle
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Re: glc knotting or contamination? [Re: Stropharis]
    #15538229 - 12/19/11 03:46 AM (12 years, 2 months ago)

It's hard to take good pictures of LCs :lol:


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OfflineSKrink
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Re: glc knotting or contamination? [Re: Kizzle]
    #15538510 - 12/19/11 07:02 AM (12 years, 2 months ago)

A glc wouldn't "knot".


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Offlinedmoney
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Registered: 12/08/11
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Re: glc knotting or contamination? [Re: Kizzle]
    #15538815 - 12/19/11 08:42 AM (12 years, 2 months ago)

  heres what mine looks like


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OfflineShamrocker
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Re: glc knotting or contamination? [Re: dmoney]
    #15539395 - 12/19/11 11:10 AM (12 years, 2 months ago)

Quote:

dmoney said:
  heres what mine looks like




Sweet! Looks like you'd need a garden hose to suck that out! Lol


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Offlinedmoney
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Re: glc knotting or contamination? [Re: Shamrocker]
    #15539741 - 12/19/11 12:47 PM (12 years, 2 months ago)

lol yeah its alittle thick,thanks to this site though i made one of them agitators with hard drive magnets and cpu fan. after using that it goes right up nicely although id like an LC syringe. larger gauge needle and 60 cc storage would be sweet


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InvisibleTranscendingLife
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Re: glc knotting or contamination? [Re: SKrink]
    #15539843 - 12/19/11 01:18 PM (12 years, 2 months ago)

Quote:

SKrink said:
A glc wouldn't "knot".




GLCs will continue to grow a bit after the extraction.
You pull a bit of nutrients from the grains w/ the mycelium laden water.
Here's a picture of a GLC 1 week after the extraction:


OP - that's an extremely small amount of GLC. I normally can pull out 99% of the water I inject.
& I can't tell anything from your pictures.
Test it on a PF cake & find out.
When taking pictures of (G)LCs, it's best to have all the lights off except a small desk light.
Put the (G)LC in front of the light & make sure your camera is on macro-mode.

dmoney - everythingmushrooms.com has the syringes you're looking for.


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Offlinethe_hatter
half mad

Registered: 04/18/10
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Last seen: 7 years, 11 months
Re: glc knotting or contamination? [Re: dmoney]
    #15539894 - 12/19/11 01:26 PM (12 years, 2 months ago)

yeah sorry for the quality of photos but as kizzle stated its tricky to get a picture of an lc and especially one that has very little left and youre trying to get a picture of the top.  but you can kind of see in the first picture white dots of mycelium forming on top of the water, this jar was actually sitting out for two months after i used it for a project and left it sitting out, which is why knots wouldnt surprise me too much.

oh well though ill shoot up some jars with it tomorrow and well know soon

oh and thanks for the picture tips TL :thumbup:


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InvisibleStropharis
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Re: glc knotting or contamination? [Re: TranscendingLife]
    #15539913 - 12/19/11 01:29 PM (12 years, 2 months ago)

Quote:

TranscendingLife said:
Quote:

SKrink said:
A glc wouldn't "knot".




GLCs will continue to grow a bit after the extraction.
You pull a bit of nutrients from the grains w/ the mycelium laden water.
Here's a picture of a GLC 1 week after the extraction:


OP - that's an extremely small amount of GLC. I normally can pull out 99% of the water I inject.
& I can't tell anything from your pictures.
Test it on a PF cake & find out.
When taking pictures of (G)LCs, it's best to have all the lights off except a small desk light.
Put the (G)LC in front of the light & make sure your camera is on macro-mode.

dmoney - everythingmushrooms.com has the syringes you're looking for.




YA DONE SON! And thats how you take a photo!


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InvisibleTranscendingLife
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Registered: 06/09/10
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Re: glc knotting or contamination? [Re: Stropharis]
    #15539926 - 12/19/11 01:31 PM (12 years, 2 months ago)

the_hatter - yea, sometimes I'll get patches of colonizing mycelium on the top of my LCs.
The jar on the right is an LC inoculated w/ the GLC on the left.
You can see the thick mycelium matting in the center of the jar on the right (you may have to super enlarge it).


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinedmoney
Stranger
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Registered: 12/08/11
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Last seen: 10 years, 4 months
Re: glc knotting or contamination? [Re: the_hatter]
    #15540315 - 12/19/11 03:00 PM (12 years, 2 months ago)

im not positive but if its growing ontop of the liquid im pretty sure thats contamination.i had a jar of LC do that and threw it away. be sure to test it on only 1 jar. i think its mold growing ontop of the liquid.


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OfflineSKrink
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Registered: 01/29/11
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Re: glc knotting or contamination? [Re: TranscendingLife]
    #15540359 - 12/19/11 03:12 PM (12 years, 2 months ago)

Quote:

TranscendingLife said:
Quote:

SKrink said:
A glc wouldn't "knot".




GLCs will continue to grow a bit after the extraction.
You pull a bit of nutrients from the grains w/ the mycelium laden water.
Here's a picture of a GLC 1 week after the extraction:






Yup they do continue to grow abit, I've had GLC syringes in the fridge that eventually started looking more like grown LCs.

I think of "knotting" as the thing that happens in a substrate prior to primordia formation-- have not been that fortunate to have it happen in any sort of LC tho. :shrug: :2c:

Cool picture skillz tho I'm gona try that out!


--------------------
:crazy2: SWEET POTATO HOME FRIES :crazy2:
:mushroom2:HOW TO USE A PENIS ENVY SPORE SWAB:mushroom2:

   

... Oh mighty masticator, salivator, vocalizer, swallower, licker biter sucker brow-knitter looker blinker rubbernecker thumber prodder up-yours fingerer ringwearer nosepicker waver drinker armlifter bodybender hipswiveler kneer springer runner ZERO::::::::OOOOOOOOO:::::::: RUN!!!


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InvisibleTranscendingLife
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Re: glc knotting or contamination? [Re: dmoney]
    #15540378 - 12/19/11 03:15 PM (12 years, 2 months ago)

Mycelium growing on top of the LC is not a sign of a contam.
I've had several LCs that grew on the top & none were contaminated.
Think of the LC somewhat like an agar dish.
The mycelium will colonize the top of the LC to find some GE & food.

The only bad LC I've had, was due to a bacteria contamination in the GLC jar that I didn't see.

& like SKrink said, knotting is the first formation of pins then primordia then pins.
You can see rhizo growth in (G)LC jars, though.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinedmoney
Stranger
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Registered: 12/08/11
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Last seen: 10 years, 4 months
Re: glc knotting or contamination? [Re: TranscendingLife]
    #15540413 - 12/19/11 03:22 PM (12 years, 2 months ago)

next time ill look closer to see what it really looks like but i like to play it safe and i expect alot of contams in my house. LC is so cheap and easy to make i just tossed it anyway. thanks for that info though! >< gotta love trial and error


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Offlinethe_hatter
half mad

Registered: 04/18/10
Posts: 753
Last seen: 7 years, 11 months
Re: glc knotting or contamination? [Re: TranscendingLife]
    #15540683 - 12/19/11 04:13 PM (12 years, 2 months ago)

Quote:

TranscendingLife said:
the_hatter - yea, sometimes I'll get patches of colonizing mycelium on the top of my LCs.
The jar on the right is an LC inoculated w/ the GLC on the left.
You can see the thick mycelium matting in the center of the jar on the right (you may have to super enlarge it).





yeah that is very similar to how mine looks just with a couple clusters of white dots.  im pretty confident its not contaminated though just must have had some nutrients from the grain as was suggested.


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