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Invisibler_borlax
The Greatest Weight
Male

Registered: 11/03/09
Posts: 63
Loc: Ursa Major
Getting & making Panaeolus Tropicalis liquid tissue syringes
    #15279519 - 10/26/11 01:46 AM (12 years, 3 months ago)

Does injecting water into an agar jar of Panaeolus Tropicalis mycelium then sucking up the water and myc seem like a decent idea to get a liquid tissue syringe to start a grain jar or malt/dex LC with? I'd prefer to put the tissue from the agar into a grain jar to make more tissue syringes from, and would rather stay away from LC due to the sugars in the tissue water - maybe helping prevent bacterial contams in grain spawn/bulk sub bags. The main reason i ask if the water in the agar jar seems like a decent idea is because i read shaking panaeolus mycelium spawn jars too much can stall them to where the myc won't recover, and this seems possibly similar to shaking a spawn jar with moving the myc from agar, to grain, to tissue syringes then to spawn jars/bulk sub bags. I'm sure i could always move a tissue sample from agar directly into grain or LC with a scalpel but this seems easier and safer on sterility for now. Thanks


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Invisibler_borlax
The Greatest Weight
Male

Registered: 11/03/09
Posts: 63
Loc: Ursa Major
Re: Getting & making Panaeolus Tropicalis liquid tissue syringes [Re: r_borlax]
    #15280290 - 10/26/11 09:11 AM (12 years, 3 months ago)

Any suggestions?


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OfflineForager
Mycophile

Registered: 02/15/11
Posts: 440
Last seen: 3 years, 1 month
Re: Getting & making Panaeolus Tropicalis liquid tissue syringes [Re: r_borlax]
    #15521243 - 12/15/11 02:54 PM (12 years, 2 months ago)

Quote:

r_borlax said:
Does injecting water into an agar jar of Panaeolus Tropicalis mycelium then sucking up the water and myc seem like a decent idea to get a liquid tissue syringe to start a grain jar or malt/dex LC with?




No need to be afraid of LC, if you've got a PC it's fairly simple!  I assume since you are working with grain, you do have a PC?  I would make up an LC (I use 1/8 cup dextrose, 1 tsp malt extract (light) to 1 quart of water, pressure cooked for ~20 min.  That is plenty long enough to get an LC sterile.  Then just get scalpel, flame sterilize the blade, and cut out a square of colonized agar medium, and quickly tranfer it to the LC (now cooled to room temp).  I would say do for the LC, easy to make your print/syringe last forever with an LC. 
Your method described above you work, but might be more difficult to do in a sterile manner than an LC.
BTW, saw you posted this a month ago, do you now have prints?  If you have Pan. tropicalis prints produced from print/syringe from a reputable vendor (so it isn't too questionable whether it is actually Pan. tropicalis), I would be very interested in getting a print.  :pm: me!


--------------------
Always looking for interesting active prints (especially Gymnopilus spp. and sclerotia producers) and cacti and poppy seeds.


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