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Happy2fly
The Champ!




Registered: 05/18/09
Posts: 1,287
Loc: The land of many thousand...
Last seen: 11 years, 1 month
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My first monotub, updated with pics!
#15454212 - 12/02/11 11:22 AM (13 years, 1 month ago) |
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I inoculated 7 WBS Jars exactly 19 days ago. At this time, 1 jar is about 80%-90% done, 1 jar is at maybe 10%, 1 jar has a couple of pea-sized spots of myc, and the rest still look like they did the day I PC'ed them.
I followed Doc's tek except for the jar lids, which I substituted felt in place of the tyvek. My plan was to follow Damion5050's coir tek, but it looks like I will not have enough spawn for a whole brick of coir.
My theory is that I forgot to shake the syringe before inoculation. What would the shroomery recommend that I do? My thoughts are that I could wait until I have 2 or 3 at 100%, then get a smaller container and only use a 1/2 brick of coir, or maybe attempt a G2G with the one that is almost finished. I also have 1 more syringe. Would it make any sense to re-inject the jars that have no signs of growth? There are no signs of contamination from what I can tell, but they were PC'ed almost 3 weeks ago...
Any advice is appreciated!
-------------------- The fire has many things to teach.
But so does the human experience.. which is like fire, sometimes you just need to stoke the coals and sometimes you just need to add a log or reposition the log matrix. But a well built fire will be much more self-sustaining than a poorly built one, and they all need love to grow. And don't fuck around or your might burn yourself. Must always respect the fire. - Shroomism
Edited by Happy2fly (02/04/12 01:44 PM)
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brand0n
large jar mad man!!


Registered: 02/09/09
Posts: 842
Last seen: 10 years, 6 months
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build a still air box or a glove box and take that one jar youve got and make it into 10.
if you dont mess up, youll at bare min get 5 good jars. you can take those 5 good jars and turn them into 25-40 jars no prob.
-------------------- That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 4 months, 24 days
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Re: WBS troubles! [Re: brand0n]
#15454295 - 12/02/11 11:56 AM (13 years, 1 month ago) |
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don't re-inject the jars that didn't take off, make new ones. When it gets down to the science of it, there is no such thing as 100% sterile. When you prep the grain you knock the contaminants down significantly, but you can't kill them all, and they have had 3 weeks to regenerate now. You don't want to re p/c them because then the grain will be overcooked, and they have lost some moisture, but you don't know how much. You could try a g-2-g with the one that colonized, or get a little pan and cut a peice off your block of coir and do a little bulk grow in a little pan
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: WBS troubles! [Re: SomeGuy] 1
#15454550 - 12/02/11 01:17 PM (13 years, 1 month ago) |
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My first WBS attempt I tried Docs tek. Nothing grew & my grains were still way too hard. Now all I do for WBS is RR's rye grain tek. Get all the sticks out, leave the other floaters (sunflower seeds). Rinse thoroughly. Soak for 18-24 hours w/ gypsum. In the same soak pot, bring the soak water & WBS to a boil. Boil for 30-60 seconds. Dump in your strainer & toss every 2-3 minutes for 60 minutes, or until the exterior of the grains is dry.
 Load your jars & PC. Remember to leave your toggle weight off your PC until you see steam coming out of the nozzle. The pressure will rise slightly, too (1-2 PSI), then put your weight on. That way you know the entire inside of the PC is all steam & not just hot air.
Here's a pic of my WBS after being PCed (they were just inoculated, too , though so they may look slightly wet)

& like the other two have said, don't re-inoculate your jars. Just take the one that's almost 100% & when it is 100% G2G that to 5-12 more jars.
Damion5050's coir tek is a great one & all I use.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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hawkington


Registered: 11/23/11
Posts: 29
Last seen: 13 years, 6 days
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seems really straight forward man, I will follow this for my first attempt.
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Happy2fly
The Champ!




Registered: 05/18/09
Posts: 1,287
Loc: The land of many thousand...
Last seen: 11 years, 1 month
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Thanks for the advice everyone, especially transcendinglife! I think I might try out your method. How much risk is there with the G2G in regard to contamination? I have never done one before, and am a little bit concerned that it's another step that can go wrong. I guess I still have another syringe though, just in case...
-------------------- The fire has many things to teach.
But so does the human experience.. which is like fire, sometimes you just need to stoke the coals and sometimes you just need to add a log or reposition the log matrix. But a well built fire will be much more self-sustaining than a poorly built one, and they all need love to grow. And don't fuck around or your might burn yourself. Must always respect the fire. - Shroomism
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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You need to make a good SAB. Here's a link to my tek: https://www.shroomery.org/forums/showflat.php/Number/13552603#13552603
& follow proper sterile procedure & be extremely quick when doing it. I follow RR's methods of G2Ging inside my SAB.
I've had one contam so far & done over 40 G2Gs in it. & I know exactly why & how I got the contam.
G2Gs were scary to me, at first, too. You'll get the hang of it & really enjoy it. I can G2G 1 jar to 12 in less time than inoculating 12 jars w/ a syringe.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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redcat
Fabulous

Registered: 11/30/09
Posts: 345
Loc: In Love
Last seen: 2 years, 1 month
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I've used TranscendingLife's WBS method of RR's rye grain tek combined with the 30 to 60 second boil and it works awesome!
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Happy2fly
The Champ!




Registered: 05/18/09
Posts: 1,287
Loc: The land of many thousand...
Last seen: 11 years, 1 month
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Re: WBS troubles! [Re: redcat]
#15455279 - 12/02/11 03:59 PM (13 years, 1 month ago) |
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Quote:
TranscendingLife said: You need to make a good SAB. Here's a link to my tek: https://www.shroomery.org/forums/showflat.php/Number/13552603#13552603
& follow proper sterile procedure & be extremely quick when doing it. I follow RR's methods of G2Ging inside my SAB.
I've had one contam so far & done over 40 G2Gs in it. & I know exactly why & how I got the contam.
G2Gs were scary to me, at first, too. You'll get the hang of it & really enjoy it. I can G2G 1 jar to 12 in less time than inoculating 12 jars w/ a syringe.
Nice! That makes me feel a bit better about it. Like I said, I have another syringe in case my G2G attempt fails. I'm gonna go for it!
Quote:
redcat said: I've used TranscendingLife's WBS method of RR's rye grain tek combined with the 30 to 60 second boil and it works awesome! 
Thats good to hear! It looks like a lot less work than Doc's tek when you remove all of the rinsing steps.
I have heard that you can use drywall for gypsum. I happen to have some drywall scraps left over from a home improvement project. Will any drywall work?
-------------------- The fire has many things to teach.
But so does the human experience.. which is like fire, sometimes you just need to stoke the coals and sometimes you just need to add a log or reposition the log matrix. But a well built fire will be much more self-sustaining than a poorly built one, and they all need love to grow. And don't fuck around or your might burn yourself. Must always respect the fire. - Shroomism
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Make sure it's not treated drywall & to remove the paper before breaking it up.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Happy2fly
The Champ!




Registered: 05/18/09
Posts: 1,287
Loc: The land of many thousand...
Last seen: 11 years, 1 month
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Thanks to the advice of TrancendingLife, my G2G was successful and I now have 7 colonized quart jars of WBS! The fastest has been done for about 10 days now, and the last one just finished in the past day or two. Now I need some more advice:
I have all of the supplies required for Damion5050's coir tek. My problem is that I have a ski vacation scheduled for Jan 11-15th, and will be out of town during that time. If I spawn my jars tomorrow, (1st available time I have) the monotub will have 14 days to colonize before I leave. When I get home, it will be 19 days. I have read that the most common time-span between spawning and introducing fruiting conditions is 10 days. I don't want to come home to a tub full of spores.
What would the shroomery suggest? Should I wait a week or so to spawn, so that it will colonize during my vacation, or spawn now and cross my fingers that I don't have to pick them after their prime?
-------------------- The fire has many things to teach.
But so does the human experience.. which is like fire, sometimes you just need to stoke the coals and sometimes you just need to add a log or reposition the log matrix. But a well built fire will be much more self-sustaining than a poorly built one, and they all need love to grow. And don't fuck around or your might burn yourself. Must always respect the fire. - Shroomism
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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I'd spawn now & introduce Fruiting Conditions right before you leave.
You'll be cutting it close on the sporing time, but you may be alright.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Happy2fly
The Champ!




Registered: 05/18/09
Posts: 1,287
Loc: The land of many thousand...
Last seen: 11 years, 1 month
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Just wanted to post an update and let you know that my grow is a success!
Here are a couple of pics that were taken about a week after introducing fruiting conditions:



The whole tub turned into a mess of mutants, and then normal pins started poking up through the gaps. I let it all grow out, and have been picking the first flush a few at a time as the veil breaks. So far I have around 3oz cracker dry, and my dehydrator is 75% full! I also have an additional 140g fresh divided up in 4 cups for a little taste-test tonight with a few friends... 
I should be picking the rest of the flush tomorrow and then doing a dunk for flush #2...
-------------------- The fire has many things to teach.
But so does the human experience.. which is like fire, sometimes you just need to stoke the coals and sometimes you just need to add a log or reposition the log matrix. But a well built fire will be much more self-sustaining than a poorly built one, and they all need love to grow. And don't fuck around or your might burn yourself. Must always respect the fire. - Shroomism
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