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InvisibleWorld Spirit
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22S, 23S-homobrassinolide
    #1544479 - 05/13/03 07:30 AM (20 years, 10 months ago)

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1544515 - 05/13/03 07:56 AM (20 years, 10 months ago)


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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1544682 - 05/13/03 09:37 AM (20 years, 10 months ago)

Thanks, you two rule!

I'll have to spend some time looking up a ref to an extraction....

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Invisiblezeta
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1547246 - 05/14/03 01:45 AM (20 years, 10 months ago)

That's extraordinary.
How hard do you suppose it would be to obtain some 22S, 23S-homobrassinolide?
It's not like you need much.. only 0.01ppm
Aren't you excited about this Anno?

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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: zeta]
    #1547278 - 05/14/03 02:07 AM (20 years, 10 months ago)

10mg is 200$ ( http://www.cidtech-research.com/cib.html )

Unfortunately I mostly forgot about this, but if I'm correct then 0.01ppm is the concentration. The actual volume used is not specified in that document. With 10mg you can get 1000l of 0.01ppm concentration.

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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1547306 - 05/14/03 02:23 AM (20 years, 10 months ago)

Right on -- they said 10^(-2) ppm = .01 ppm

Thx for the link!

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Edited by micro (05/14/03 02:34 AM)

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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: micro]
    #1547975 - 05/14/03 11:33 AM (20 years, 10 months ago)

Green beans contain lots of different hb's, including the specific one gartz used.

The best organic source for 22s, 23s-hb is rape seed pollen. Rape seed is commonly grown as a cover crop by organic farmers, so seeds are easy to get.

I would think that collecting the pollen in a plastic bag from a pollinating plant would be very simple, and provide a nice supplement to play with, although you wouldn't really know exact concentrations and stuff.


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"From a certain point of view"
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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1547993 - 05/14/03 11:44 AM (20 years, 10 months ago)

I think now is the time where rape is in bloom.

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Offlinecomario2
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1548319 - 05/14/03 01:54 PM (20 years, 10 months ago)

good to know the time, but where do we find the blooms, that is the question :laugh: :grin: 


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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: comario2]
    #1548330 - 05/14/03 01:58 PM (20 years, 10 months ago)

Large fields, yellow flowers.


Edited by Anno (05/14/03 01:59 PM)

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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1548490 - 05/14/03 02:56 PM (20 years, 10 months ago)

Get A couple trash bags of that stuff Anno!


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1550009 - 05/15/03 01:01 AM (20 years, 10 months ago)

"I think now is the time where rape is in bloom."

Ha! Yeah -- Spring, love is in the air!

I found some ref's to some journal articles for a synthesis and extraction but I left them in work -- I'll post them tomorrow.

--
Micro



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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: micro]
    #1550360 - 05/15/03 07:18 AM (20 years, 10 months ago)

T. C. McMorris, P. A. Patil, R. G. Chavez, M. E. Baker & S. D. Clouse, Synthesis and biological activity of 28-homobrassinolide and analogues,
Phytochemistry 36(3): 585-589, 1994

K. Gamoh, I. Yamaguchi & S. Takatsuto, Rapid and selective sample preparation for the chromatographic determination of brassinosteroids from plant
material using a solid-phase extraction method, Analytical Sciences 10: 913-917, 1994

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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InvisibleWorld Spirit
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1597461 - 06/01/03 12:56 AM (20 years, 9 months ago)

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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1597901 - 06/01/03 08:56 AM (20 years, 9 months ago)

Yes, but look at my post above. With 10mg you can get 1000l of 0.01ppm concentration.

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Offlineanotherdirtywop
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1597970 - 06/01/03 10:09 AM (20 years, 9 months ago)

Bee pollen is another source of homobrassino.......

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InvisibleWorld Spirit
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1598677 - 06/01/03 03:52 PM (20 years, 9 months ago)

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Anonymous

Post deleted by Papaver [Re: World Spirit]
    #1598776 - 06/01/03 04:36 PM (20 years, 9 months ago)


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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1600258 - 06/02/03 12:55 AM (20 years, 9 months ago)

Quote:

So you get 10mg of this stuff and make 1000ml of liquid?




Not 1000ml - You would get 1000l of this liquid!!! (0.01ppm = 0.01 mg/kg)

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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: anotherdirtywop]
    #1601299 - 06/02/03 12:08 PM (20 years, 9 months ago)

Quote:

Bee pollen is another source of homobrassino.......



I've never seen anything more than speculation showing that HB or it's relatives are really present in bee pollen. Not to mention concentrations, and variation in brand/source/season etc.

I've seen experiments that indicate that bee pollen has a similar effect, but nobody has ever shown that is actually a homobrasinosteroid responsible for these results.

At least not that I've seen. If anybody has a hard reference, please post it to clear up this obviously un-scientific ambiguity.

Also, a European has told me that their health stores carry "mixed-pollen" which has given similar results to homobrassinosteroids and bee pollen in experiments. So if you're in Europe and can't find bee pollen, try the mixed pollen.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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OfflineRustik
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1602654 - 06/02/03 07:54 PM (20 years, 9 months ago)

Quote:

Bee pollen is another source of homobrassino.......




Umm.. Bees make pollen?  :wink: If the bee picks the pollen up from a rapeseed, then perhaps it does. If not, then it might not. But I KNOW bees don't MAKE pollen..

lol :P 


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OfflineAnnoA
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much, much pollen [Re: ]
    #1603382 - 06/03/03 12:04 AM (20 years, 9 months ago)

>Could you just use the pollen or would it need to be extracted from the pollen?

I read that you need hundreds of kilos of rape pollen to be able to extract the amounts needed.

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Invisiblemycofile
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Re: much, much pollen [Re: Anno]
    #1603949 - 06/03/03 08:51 AM (20 years, 9 months ago)

Where did you read that anno? The reference I saw showed high concentrations of brassinosteroids in rape seed pollen. Granted, that was a while back, and I don't remember where it was, but I was always under the impression that it was one of the best organic sources.

Also, in regards to bee pollen Fungus Maximus (I think that's who) has shown definite increases in growth in liquid cultures with bee pollen. He actually even had a liquid culture form a fruit body (not from the liquid itself, but from a mass of mycelium floating on top of it). Now, like I said, that could be due to some unknown chemical, but it certainly sounds like the effects of a brassinosteroid....


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

Edited by mycofile (06/03/03 08:53 AM)

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OfflineAnnoA
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Re: much, much pollen [Re: mycofile]
    #1603996 - 06/03/03 09:17 AM (20 years, 9 months ago)

>Where did you read that anno?

Believe me, a week after I read it I searched again for it for almost an hour, to no avail...

I can?t find the source anymore, but it was in the internet.

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1604073 - 06/03/03 09:46 AM (20 years, 9 months ago)

Ok, so the motto is "never give up"

Here:
http://www.chm.bris.ac.uk/motm/brassinolide/brassinolidej.htm

"The huge effort of processing 40 kg of collected bee pollen of Brassica napus L. yielded 4 mg of a crystalline material which was identified as (22R,23R,24S)-2a,3a,22,23-tetrahydroxy- 24-methyl-B-homo-7-oxa-5a-cholestan-6-one.[3] "

More:
http://members.tripod.com/~mzullo/sourcebrassino.htm

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OfflineODhaze
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1604406 - 06/03/03 11:47 AM (20 years, 9 months ago)



i can get a few fresh pounds of this stuff if anyone wants any of it...

i am about 95% sure that it is rape. judging from the pictures..

there is some growing next to where i work, PM me if interested, it would be worth a shot at extracting the brassinosteroids i think.


--------------------
I believe i will go out to the seashore, let the waves wash my mind. Gunna open up my head now just to see what i can find...

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: ODhaze]
    #1604436 - 06/03/03 11:53 AM (20 years, 9 months ago)

the pollen only, though....

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OfflineODhaze
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1604458 - 06/03/03 12:00 PM (20 years, 9 months ago)

shit i didnt realize it was 40KG of the pollen alone,

thats a lot of work,


--------------------
I believe i will go out to the seashore, let the waves wash my mind. Gunna open up my head now just to see what i can find...

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: ODhaze]
    #1604464 - 06/03/03 12:02 PM (20 years, 9 months ago)

indeed.

still, try to get at least a few grams.

Edited by Anno (06/03/03 12:02 PM)

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