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InvisibleWorld Spirit
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22S, 23S-homobrassinolide
    #1544479 - 05/13/03 09:30 AM (13 years, 6 months ago)

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1544515 - 05/13/03 09:56 AM (13 years, 6 months ago)



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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1544682 - 05/13/03 11:37 AM (13 years, 6 months ago)

Thanks, you two rule!

I'll have to spend some time looking up a ref to an extraction....

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Invisiblezeta
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1547246 - 05/14/03 03:45 AM (13 years, 6 months ago)

That's extraordinary.
How hard do you suppose it would be to obtain some 22S, 23S-homobrassinolide?
It's not like you need much.. only 0.01ppm
Aren't you excited about this Anno?


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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: zeta]
    #1547278 - 05/14/03 04:07 AM (13 years, 6 months ago)

10mg is 200$ ( http://www.cidtech-research.com/cib.html )

Unfortunately I mostly forgot about this, but if I'm correct then 0.01ppm is the concentration. The actual volume used is not specified in that document. With 10mg you can get 1000l of 0.01ppm concentration.


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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1547306 - 05/14/03 04:23 AM (13 years, 6 months ago)

Right on -- they said 10^(-2) ppm = .01 ppm

Thx for the link!

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Edited by micro (05/14/03 04:34 AM)


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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: micro]
    #1547975 - 05/14/03 01:33 PM (13 years, 6 months ago)

Green beans contain lots of different hb's, including the specific one gartz used.

The best organic source for 22s, 23s-hb is rape seed pollen. Rape seed is commonly grown as a cover crop by organic farmers, so seeds are easy to get.

I would think that collecting the pollen in a plastic bag from a pollinating plant would be very simple, and provide a nice supplement to play with, although you wouldn't really know exact concentrations and stuff.


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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1547993 - 05/14/03 01:44 PM (13 years, 6 months ago)

I think now is the time where rape is in bloom.


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Offlinecomario2
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1548319 - 05/14/03 03:54 PM (13 years, 6 months ago)

good to know the time, but where do we find the blooms, that is the question :laugh: :grin: 


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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: comario2]
    #1548330 - 05/14/03 03:58 PM (13 years, 6 months ago)

Large fields, yellow flowers.



Edited by Anno (05/14/03 03:59 PM)


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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1548490 - 05/14/03 04:56 PM (13 years, 6 months ago)

Get A couple trash bags of that stuff Anno!


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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1550009 - 05/15/03 03:01 AM (13 years, 6 months ago)

"I think now is the time where rape is in bloom."

Ha! Yeah -- Spring, love is in the air!

I found some ref's to some journal articles for a synthesis and extraction but I left them in work -- I'll post them tomorrow.

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Invisiblemicro
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Re: 22S, 23S-homobrassinolide [Re: micro]
    #1550360 - 05/15/03 09:18 AM (13 years, 6 months ago)

T. C. McMorris, P. A. Patil, R. G. Chavez, M. E. Baker & S. D. Clouse, Synthesis and biological activity of 28-homobrassinolide and analogues,
Phytochemistry 36(3): 585-589, 1994

K. Gamoh, I. Yamaguchi & S. Takatsuto, Rapid and selective sample preparation for the chromatographic determination of brassinosteroids from plant
material using a solid-phase extraction method, Analytical Sciences 10: 913-917, 1994

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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1597461 - 06/01/03 02:56 AM (13 years, 6 months ago)

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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1597901 - 06/01/03 10:56 AM (13 years, 6 months ago)

Yes, but look at my post above. With 10mg you can get 1000l of 0.01ppm concentration.


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Offlineanotherdirtywop
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1597970 - 06/01/03 12:09 PM (13 years, 6 months ago)

Bee pollen is another source of homobrassino.......


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InvisibleWorld Spirit
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Re: 22S, 23S-homobrassinolide [Re: zeronio]
    #1598677 - 06/01/03 05:52 PM (13 years, 6 months ago)

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Anonymous

Post deleted by Papaver [Re: World Spirit]
    #1598776 - 06/01/03 06:36 PM (13 years, 6 months ago)



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Offlinezeronio
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Re: 22S, 23S-homobrassinolide [Re: World Spirit]
    #1600258 - 06/02/03 02:55 AM (13 years, 6 months ago)

Quote:

So you get 10mg of this stuff and make 1000ml of liquid?




Not 1000ml - You would get 1000l of this liquid!!! (0.01ppm = 0.01 mg/kg)


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Invisiblemycofile
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Re: 22S, 23S-homobrassinolide [Re: anotherdirtywop]
    #1601299 - 06/02/03 02:08 PM (13 years, 6 months ago)

Quote:

Bee pollen is another source of homobrassino.......



I've never seen anything more than speculation showing that HB or it's relatives are really present in bee pollen. Not to mention concentrations, and variation in brand/source/season etc.

I've seen experiments that indicate that bee pollen has a similar effect, but nobody has ever shown that is actually a homobrasinosteroid responsible for these results.

At least not that I've seen. If anybody has a hard reference, please post it to clear up this obviously un-scientific ambiguity.

Also, a European has told me that their health stores carry "mixed-pollen" which has given similar results to homobrassinosteroids and bee pollen in experiments. So if you're in Europe and can't find bee pollen, try the mixed pollen.


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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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