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OfflineFungiWrangler
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preventing sporulation for commercial production
    #15342829 - 11/09/11 01:03 AM (12 years, 3 months ago)

I was wondering if anyone had experience with detecting/forcing fungi which do not produce spores consistently.  I imagine this would be something commercial farmers think a lot about as they deal with all kinds of problems from the spores.  I am interested in selecting a sterile Paecilomyces tenuipes strain. 

The two methods which would be pretty rapid that I could think of would be:
Mutating a spore solution in UV light and fruiting many small samples of the surviving colonies
or
more preferably plating many colonies on agar and looking at them under a microscope to select the ones which are lacking in a certain morphological feature.  I just have no idea which feature corresponds to no conidia production during fruiting without examining a sterile strain first. 

Are there any chemical treatments that will render the organism sterile through many transfers but still vigorous and functioning?


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Offline30rack
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Re: preventing sporulation for commercial production [Re: FungiWrangler]
    #15352427 - 11/10/11 08:08 PM (12 years, 3 months ago)

1-conidiation is asexual, and thus does not come from "mushrooms", but rather specialized structures called conidiophores.

The only way I know how to mutate genes is through the use of agrobacterium, and that is way beyond the scope of a home cultivator.

random mutations, whether they come by way of microwaves or whatever other method you want is a shot in the dark. there are many thousands of base pairs in a fungal genome. It is in theory possible I guess.

my opinion would be to simply harvest as soon as the cap opens, before spores are discharged in large quantities.


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OfflineRogerRabbitM
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Re: preventing sporulation for commercial production [Re: FungiWrangler]
    #15353977 - 11/11/11 03:18 AM (12 years, 3 months ago)

It's trial and error.  If you isolate a thousand strains of a species, you'll likely find a sporeless strain in the mix.  If you isolate several thousand strains of that species, you'll likely find a sporeless strain which also has commercial production qualities.
RR


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Invisiblemycoelf
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Re: preventing sporulation for commercial production [Re: RogerRabbit]
    #15354543 - 11/11/11 08:49 AM (12 years, 3 months ago)

My first strain of cubes was a sporeless strain of golden teacher, that I received from my teacher, he subcultured many generations of the same strain, never producing spores until the 4th or more flush. How is this accomplished? He prayed over the petri dish asking the Myc to not produce spores

Take that for what you will. What I know is that that strain remained spore less for about 4 generations and then reverted back to its regular state

:aliendance:


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Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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Invisibleflameclown
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Re: preventing sporulation for commercial production *DELETED* [Re: mycoelf]
    #15354703 - 11/11/11 09:28 AM (12 years, 3 months ago)

Post deleted by flameclown

Reason for deletion: [this post is damn old]


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Invisiblemycoelf
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Re: preventing sporulation for commercial production [Re: flameclown]
    #15355755 - 11/11/11 01:42 PM (12 years, 3 months ago)

Quote:

flameclown said:
Its likely that it wasnt a mono culture then.



No, indeed, a Non sectoring isolate derived from a clone


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Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

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OfflineFungiWrangler
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Re: preventing sporulation for commercial production [Re: mycoelf]
    #15356431 - 11/11/11 03:58 PM (12 years, 3 months ago)

RR: Do you think my chances of finding a commercial production strain which does not produce conidia could be found in <100 samples of surviving strains if conidia was mutated to the point of ~98% lethality?

ps. this is not a basidiomycete so it may be different then the research you are used to.


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Edited by FungiWrangler (11/11/11 10:38 PM)

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