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OfflineOmegaRed
Myco-Floydianenthusiast

Registered: 04/16/03
Posts: 193
Loc: right behind you
Last seen: 12 years, 8 months
Newbie kicking self in ass.
    #1534390 - 05/09/03 01:20 PM (13 years, 6 months ago)

My friend just completed his first innoc. experiment and he is now kicking himself in the ass for a stupid mistake he made and was warning others against this. I know a lot of you will probably laugh at this but some of you out there are newbies so read up and don't make the same mistake my friend did. If at the end of your jars you still have innoculant left in the syring DO NOT RANDOMLY PICK OUT JARS TO GIVE ANOTHER SHOT OF INNOCULANT TO. Take the ones you innoculated last and go backwards from there. By selecting jars at random my friend contaminated half of his jars with lipstick mold that he picked up somewhere towards the end of the initial innoculation.


--------------------
Hey Mr. Tamborine Man play a song for me,
I'm not sleepy and there's no place I'm going to.
Hey Mr. Tamborine Man play a song for me,
In the jingle jangle morning I'll come following you.


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Invisibleafoaf
CEO DBK?
 User Gallery

Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
Re: Newbie kicking self in ass. [Re: OmegaRed]
    #1534399 - 05/09/03 01:23 PM (13 years, 6 months ago)

flame sterilization of the needle between
every hole on every jar is also good practice
to get into.

cutting corners will usually cost more pain than
you would have saved had things worked out
ideally.


--------------------
All I know is The Growery is a place where losers who get banned here go.


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OfflineJohnnyRespect
Nomadic Wanderer
Registered: 04/17/03
Posts: 676
Loc: East Coast
Last seen: 13 years, 5 months
Re: Newbie kicking self in ass. [Re: afoaf]
    #1534406 - 05/09/03 01:25 PM (13 years, 6 months ago)

My biggest "newbie" mistake was using my "disinfectant" spray instead of my "misting" spray for my casings. The difference? My disinfectant was 1:9 bleach:water. Took me about a week to realize it smells really bleachy, and by then it was all dead.


Jr


--------------------
As I felt the soft cool mud squish between my toes, I thought, Man, these are not very good shoes!


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Offlinebansheee
Novice

Registered: 04/25/03
Posts: 107
Loc: where its sunny
Last seen: 11 years, 9 months
Re: Newbie kicking self in ass. [Re: afoaf]
    #1534408 - 05/09/03 01:26 PM (13 years, 6 months ago)

If you used enough in the first place you probably arejust messing with the water content. I would do it right the first time and save a lil inoculent in case you lose everything.


--------------------
Knowledge speaks, but wisdom listens. - Jimi Hendrix


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Anonymous

Re: Newbie kicking self in ass. [Re: bansheee]
    #1534639 - 05/09/03 02:43 PM (13 years, 6 months ago)

Yeh I probably screwed up my first innoc job. I get everything pretty sterile, I have a small heat pad in the chamber keeping it at low 80s. But I'm pretty sure nothing is going to grow (although, I would like some contams to grow just to see what they look like in RL). I left the substrates out for awhile after sterilized to see if there were any contams and there weren't, so probably nothing will grow. It was all so hectic feeling, I was rushing to get the needle in and out, I didn't know what to do with the jars once innoculated. Well I'll check on them in like a week to see what fun stuff have emerged. Meanwhile I've given up growing any legal mushrooms. But a friend of mine is going to use the rest of my materials to try again with another six jars.


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Offlinejakeholman
crotch rocketsrule!

Registered: 02/24/03
Posts: 503
Last seen: 9 years, 9 months
Re: Newbie kicking self in ass. [Re: ]
    #1535250 - 05/09/03 06:28 PM (13 years, 6 months ago)

hey johnny-supposedly a bleach water mix of 200:1 is good for everything from killin cobweb, to sprayin casings to dunking cakes, and does no harm
Jake


--------------------
The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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