100% colonization? can you EVER tell? - Mushroom Cultivation

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Shop: ½ Pint Jars, Agar, Rye Grain

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I_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state

100% colonization? can you EVER tell?

#1534227 - 05/09/03 11:59 AM (14 years, 3 months ago)

What does a rye berries jar look like inside when it is 100% colonized? my friend waited til every outer surface was covered in fuzzy white growth to break it up and spawn, but the inside had tons of exposed surface! AND, very little of the insides of the berries was colonized. Does 100% colonization mean each berry's inside is colonized by mycelium too??

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Be Here Now

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Room4Shroom
Mr. Nice Guy

Registered: 01/15/03
Posts: 1,195

Re: 100% colonization? can you EVER tell?

[Re: I_Stain_Blue]
#1534231 - 05/09/03 12:01 PM (14 years, 3 months ago)

Yea - every single part of the substrate should be white...EVERY part

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I_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state

Re: 100% colonization? can you EVER tell?

[Re: Room4Shroom]
#1534234 - 05/09/03 12:01 PM (14 years, 3 months ago)

so, once the outside layer is colonized, how can you tell or be sure?

my friend just had 12/13 jars contaminated!! :blush:

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NFNF
omghi2u

Registered: 04/03/03
Posts: 247
Last seen: 13 years, 9 months

Re: 100% colonization? can you EVER tell?

[Re: I_Stain_Blue]
#1534235 - 05/09/03 12:02 PM (14 years, 3 months ago)

Quote:

What does a rye berries jar look like inside when it is 100% colonized? my friend waited til every outer surface was covered in fuzzy white growth to break it up and spawn, but the inside had tons of exposed surface! AND, very little of the insides of the berries was colonized. Does 100% colonization mean each berry's inside is colonized by mycelium too??

Yep. You gotta wait it out.

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sup d00dz

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Room4Shroom
Mr. Nice Guy

Registered: 01/15/03
Posts: 1,195

Re: 100% colonization? can you EVER tell?

[Re: NFNF]
#1534240 - 05/09/03 12:06 PM (14 years, 3 months ago)

12 out of 13?! What the hell...did your friend just completely forget that he's trying to grow a fungus and that things need to be sterilized first?
Anyway...after inoculation you should start to see growth within a few days...when about 5% of the jar is colonized, shake the shit out of it...then when 50% of it is colonized, do the same...then, by the time it reaches 100%, it will uniformally be colonized throughout.
~Peace

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I_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state

Re: 100% colonization? can you EVER tell?

[Re: Room4Shroom]
#1534246 - 05/09/03 12:10 PM (14 years, 3 months ago)

yeah, 12/13... it was his first try.
all the berries were soaked for 24 hours before being pressure cooked for 45 minutes at 15 psi. They all got the same bacillus infection, greasy gross rye berries. It sucked, too, because all the jars were like half colonized. It's so sad to throw it all away!!

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Room4Shroom
Mr. Nice Guy

Registered: 01/15/03
Posts: 1,195

Re: 100% colonization? can you EVER tell?

[Re: I_Stain_Blue]
#1534252 - 05/09/03 12:12 PM (14 years, 3 months ago)

yea - i definitely know what you mean...sounds to me like he had a contam. syringe. Maybe your friend should invest in some agar and test that shit out before inoculating so many jars at once and losing all those supplies. Besides, it's really cheap - but that's just an idea.

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e7c
xyz

Registered: 04/29/03
Posts: 93

Re: 100% colonization? can you EVER tell?

[Re: Room4Shroom]
#1534254 - 05/09/03 12:12 PM (14 years, 3 months ago)

does shaking the rye berries into the polyfill vent create a possibility of contamination? or is that generally seen as not a problem

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I_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state

Re: 100% colonization? can you EVER tell?

[Re: e7c]
#1534263 - 05/09/03 12:17 PM (14 years, 3 months ago)

my friend did a bunch of half-pint jars and they all went okay .. shaking into the polyfill seems to do nothing negative

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