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kiss_the_sky03
human being



Registered: 08/19/05
Posts: 1,428
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Metabolite before Myc Growth?!
#13086161 - 08/22/10 11:37 AM (13 years, 6 months ago) |
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Hey guys,
I'm doing the pf tek. Done it plenty of times, so this isn't my first rodeo. However, there seems to be quite a bit of what I think is Myc Metabolites in one of my sets of jars. I'm talking quite a bit in all the jars....
My question is, is it possible for the metabolites to show up before any noticeable white myc is seen? There is no noticeable smells after about 3 days and it appears that some myc could actually be forming now.
What are the chances this is metabolites and not some gnarly contam?
Sorry, no pics. Jars aren't in any incubation chamber, the house temp hovers around 80 degrees. The batch with the "myc metabolites" did seem to have a bit too much water in it pre-sterilization.
What do the experts think? Any help is greatly appreciated
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Mad_Hatter2004
Surfista Amigo



Registered: 06/19/04
Posts: 2,298
Loc: Somewhere in teh Northern...
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How can there be metabolites if there is no mycelium growing?!
Please go into greater detail of what it looks like.....but this thread sucks without pics...without pics all we can do is guess and as you know a guess is just a guess,whether it's an educated guess or not.
If anything,you saying that the batch with "metabolites" did seem to have a bit too much water in it pre sterilization then this leads me to believe that could possibly be wet spot/bacteria.
Pictures can say 1,000 words....get pics up.
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7 days without waves makes one weak!
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TwistedColors
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Registered: 10/20/11
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Last seen: 12 years, 2 months
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I don't know if its too late, but I would be interested in the outcome of your jars I have noticed a very similar thing in my current grow. Any help is appreciated.
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TwistedColors
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Registered: 10/20/11
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Last seen: 12 years, 2 months
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To anybody reading this thread and are upset that those who created it feel as if a reply is too much I would like to say that any jars that had the metabolites later showed growth, I believe I just had them stored at too high of a temp.
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rony-pony
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Registered: 03/17/11
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Last seen: 11 years, 9 months
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After inoculation of of some BRF jars and some rye bags (syringes from the same print) initial colonisation looked good. After two weeks or so a yellow/rust colour started to appear in both jars and bags. In the bags there seems to be yellow liquid at the bottom. In the jars there is no surplus liquid and the yellow growth looks dry. I have tried to read what I can so apologies if this has already been explained. I was wondering could it be harmful to open? Should put the jars in boiling water for a couple of hours? Might they be alright after all - metabolites or 'myc piss' - never heard of this one. It seems all jars and bags are sitting at about 50-60% colonisation and not going any further. Any suggestions?
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Metabolite before Myc Growth?! [Re: rony-pony]
#15317280 - 11/03/11 11:25 AM (12 years, 3 months ago) |
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Quote:
There is no noticeable smells after about 3 days and it appears that some myc could actually be forming now.
Obviously, the recipe wasn't followed and you used too much water when making up the jars. Metabolites will not build up until after full colonization. Condensation often forms as the mycelium grows due to the heat produced within the jar. However, before noticeable mycelium forms, the only possible cause is too much water in the mix at makeup.
Quote:
Might they be alright after all - metabolites or 'myc piss' - never heard of this one. It seems all jars and bags are sitting at about 50-60% colonisation and not going any further.
Mycelium has no urinary tract and does not piss. If you find a trusted cultivator claiming mycelium urinates, post a link and I'll pull his tag.
I suspect bacteria contamination, based on the yellowish liquid, especially if it's a greasy slime. Toss out and start over. To be safe, toss the jars into a pot of water and bring to a boil for at least an hour before opening to dump out the contents. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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rony-pony
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Registered: 03/17/11
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Re: Metabolite before Myc Growth?! [Re: RogerRabbit]
#15317406 - 11/03/11 11:59 AM (12 years, 3 months ago) |
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Thanks RR, I was kidding myself on thinking there was going to be another answer, boil them up and start again it is!
Given that all the syringes came from the same print, would the print itself be a likely cause? Is it possible it were the syringes/needles? - I had used the syringes before but cleaned before use(Amateur I know but I had no others at that time). However, as all the jars and the bags have the same symtoms it would lead me to think it was the print itself, given there were several syringes used? Also I take it that it best not to smell suspected contams?
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rony-pony
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Registered: 03/17/11
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Re: Metabolite before Myc Growth?! [Re: rony-pony]
#15317493 - 11/03/11 12:25 PM (12 years, 3 months ago) |
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Does anyone know of anywhere I can view pictures of yellow moulds / bacteria infetions?
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