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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Invisiblevitamink
This weathersucks
Registered: 10/15/02
Posts: 104
Loc: Steers and Queers
My pets current situation (plus pics)....now what
    #1529377 - 05/08/03 01:24 AM (13 years, 9 months ago)

Ok. So here goes nothing. My pet has tried 2 times already with no sucess. Each time My pet only prepared 2 jars. My pet followed PF to the letter with the exceptions of certain details such as NOT tamping down, etc. Both time the result was only 2 jars with no growth that ended up smelling like a nasty sweaty jock strap which must've been the brf going bad after 2 weeks of sitting at room temp. The jar were sterilized properly. 45mins(sometimes a bit longer) in a pressure steamer at low heat. My pet suspects that the age of spores was at the root of the problem. They were over 6 months old.

This time, a total of 1260ml of verm was mixed with 360ml of brf along with an equal part of water. Not too wet, not too dry. All steps to stay sterile have been followed as closely as possible.

Anyone have any thoughts?

Also, a few questions. My pets pressure cooker can only fit 3 jars at a time.
Can the substrate mix be frozen before and after the steaming in order to be inoculated all at the same time?

Also, where should my pet inoculate the other odd shaped containers? Along the edge just like the jars?

What other steps should be taken in order to ensure that there will be at least some sort of growth at all?

My pet is using the B+ strain from a BRAND NEW 30ml syringe.

Please refer to the pics for any other information.

THANKS!!!



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OfflineGoodOlBattonAss
Stranger

Registered: 03/31/03
Posts: 333
Last seen: 13 years, 1 month
Re: My pets current situation (plus pics)....now what [Re: vitamink]
    #1529458 - 05/08/03 01:49 AM (13 years, 9 months ago)

>"Can the substrate mix be frozen before and after the steaming in order to be inoculated all at the same time?"

Should last at least a couple of weeks in the fridge...not too sure about freezing

>"Also, where should my pet inoculate the other odd shaped containers? Along the edge just like the jars?"

Unless you can find a better spot...That's what I'd do.

>"What other steps should be taken in order to ensure that there will be at least some sort of growth at all?"

Provide air exchange with Tyvek or Poly-fil. I have noticed very slow growth with sealed jars.


--------------------


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Anonymous

Re: My pets current situation (plus pics)....now what [Re: vitamink]
    #1529478 - 05/08/03 01:53 AM (13 years, 9 months ago)

1. If you put a nice tinfoil lid over the jars, they should be airtight and sterile, so you can keep them in the fridge for a day, I think freezing in this situation would be overkill. When you take them out of the fridge, give them a good wipe down with some alchohol, replace that tin foil lid you used also, its got all sorts of nasties on it.

2. I think you should innoculate everything but the bowl like the other jars. The bowl however has a much bigger surface, I would make 4 on the side and two toward the center. Someone will probably suggest 4 on the sides and one in the center.

3. As long as there is enough water in there and the temps are good, they should grow.

Tell your pet to have fun and kick contams nasty mold ass.


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Invisiblevitamink
This weathersucks
Registered: 10/15/02
Posts: 104
Loc: Steers and Queers
Re: My pets current situation (plus pics)....now what [Re: ]
    #1529573 - 05/08/03 02:29 AM (13 years, 9 months ago)

Well. There are now 3 jars that have been sterilized(not shown in the pictures).
They have been place in the fridge along with all the other jars and bowls in the pics. There isnt any food(my pet is broke) in the fridge so the entire inside of the fridge was sprayed down completely with lysol. All the other jars and bowls will be sterilized and inoculated(hopefully) by tomorrow night.

My pet just wants to see SOME small signs of growth this time around. An actual colonization and fruiting would be utterly outstanding.

Keep any suggestions coming.

thanks


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InvisibleMagashM
Da Bud Guru
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Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: My pets current situation (plus pics)....now what [Re: vitamink]
    #1529965 - 05/08/03 05:49 AM (13 years, 9 months ago)

Grow Log
Tek : PF
Strain : South American [SA]
Spores : Came from homemade prints. 36 total jars to be injected.
Substrate :Brown Rice Flour [BRF] and Vermiculite [Verm]
---------------------------------------------------------------------------------------------------
10:15 PM 3/4/03 : Project is started. Jar prep is just putting small holes in the lids for the spore syringe and then taping them closed until inoculation. Tape used is the standard black electrical tape. First batch of jars is mixed. The mix used is 2 parts verm, 1 part water, and 1 part brf. [ This mix is the max fruiting formula by PF.] Mix is put into 1/2 pint canning jars lightly, not packed in, and left it airy. Put substrate in until it reaches the threads of the jar, then add dry verm the rest of the way.
Jars are PCd at 10psi or 240f for a period of 45 minutes. Then jars were left to sit overnight to cool.


8:39 PM 3/5/2003 : First batch of jars injected. (24 jars)

10:04 AM 3/6/2003 : Incubator is running to low 77F. Going to turn it up today.


5:45 PM 3/6/2003 : Second batch of jars is in the PC will be injected tomorrow. Turned up incubator runs at 83F. 86F is the best and if the incubator is 83F and myc growing in the jars is suppose to create a little heat I figure this should work out well.

1:11 PM 3/7/2003 : Second batch of jars is done. (12 jars)

12:10 PM 3/9/2003 : Jars are doing good, no signs of contams


2:54 AM 3/19/2003 : Tape removed from the holes today and coffee filters put over the top of the jars for added protection. Holes were opened so the CO2 in the jars could escape. I gave 12 jars to a friend who kept fucking up. 24 jar?s about 75%done.


12:13 PM 3/22/2003 : Well it's Saturday and the jars will probably be done by the 25th. So it was 36 jars. 12 were given to a friend, 24 done by Tuesday.

10:26 PM 3/23/2003 : 15 jars were done enough to put into dunk. I dunk and cold shock before birthing and after every flush. The other 9 jars have a spot the size of a pea that has to be covered so I'll do those on the 25th.


7:17 PM 3/24/2003 : 15 jars put into the pod. Put in and put a layer of moist verm on top. With geolite I just put the cakes on it. With some other chambers I put the cake on a lid filled with verm. This is done to give the shrooms on the bottom and the top of the cake some added moisture.
The other 9 jars just finished so I'm going to give them a couple more days to make sure the middle of the cake is done.


4:57 PM 3/27/2003 : 5 More cakes birthed today, 4 left going to do them tomorrow or 3/29/2003

3:58 PM 3/29/2003 : Well I lost another cake. My wife's little 1 cake pod made from a 2 liter and a small fish pump and a little geolite used up her last cake so I gave her a SA to grow. The last 3 are in the cold shock dunk.
So the total is 36 jars done all colonized with no contams. 12 given to a friend, 1 to the wife, 23 mine

12:08 PM 3/30/2003 : Last of jars put in

12:02 PM 3/31/2003 : The first 15 cakes are pinning nicely and some have shrooms coming from the bottom.


11:01 AM 4/3/2003 : Things look to be going well.


1:02 AM 4/4/2003 : Took the first flush from four of the cakes. More will be done later today.




--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflineSmiley_Riley
kinda newbie
Registered: 02/24/03
Posts: 64
Last seen: 12 years, 8 months
Re: My pets current situation (plus pics)....now what [Re: Magash]
    #1530263 - 05/08/03 10:30 AM (13 years, 9 months ago)

Noice...


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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