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OfflineDodongo
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Questions about Turbinado LC tek
    #15249506 - 10/19/11 08:56 PM (13 years, 2 months ago)

I'm about to do agar's TURBINADO LC tek, and bought a big bag of it instead of the packets.

It says to use a 5 gram packet per jar, should I just weigh this out on a digital scale I've got, or is there a measuring spoon it amounts to? Also this tek is for pint size jars, correct?

Any other tips would be appreciated as this is my first time doing a LC. Thanks!


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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InvisibleViruk
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15249650 - 10/19/11 09:31 PM (13 years, 2 months ago)

Use the digital scale, spoons are inaccurate.
Then just weigh out 4-6% weight. 1 pint is about a half liter, so 500ml x .04% = 20g you need for each pint at a 4% mixture.
10% and above is toxic to growing mycelium, 4% is reported as the best, but some people say they get better growth from 5-6% or more.
I would use 4% right now, it's very clear, and works perfectly. Enjoy!

Later on you can experiment with stronger mixtures if you want, or weaker, to dial in what works best with your sugar.

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Viruk]
    #15250015 - 10/19/11 10:51 PM (13 years, 2 months ago)

I just realized that I don't have a measuring cup with ml on it, just working with cups, half cups, etc. at the moment.

Looks like 500ml = ~2.11 cups. Should I use 2 cups, and then 18.9g of sugar, since 2 cups is 473.176473 ml x .04 = 18.92?

Also, what psi do I PC at? It says to for 12 minutes, is this correct in your experience?

One more question, all I have is 2ml of a spore solution left, should I just prepare one of these jars or is that enough for two/more?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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Offlineigigi
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15250077 - 10/19/11 11:09 PM (13 years, 2 months ago)

When I made my raw sugar LC.. the packages actually turned out to have 4g of sugar in them, 5 w/ the paper package.  FYI


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: igigi]
    #15250091 - 10/19/11 11:12 PM (13 years, 2 months ago)

Yeah, just read about that in another thread actually. Well I'm wanting to get these jars done so I can get to bed, got class tomorrow and it's midterms week.

Does anybody know whether the calculations I made should be fine? And what psi to PC at (I'm assuming 15psi)?

Also if I have 2cc how many pint jars can/should I do?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15252811 - 10/20/11 04:06 PM (13 years, 2 months ago)

Decided to just call it a night and wait until today to do the LC. Can somebody please answer my questions before I get started?

1. Will the calculations I made do well for the amount of turbinado and water? I read in another thread "250ml water to 1-2 packets (5-10 grams) 1 pint jar", and not to fill the jars more than half full.

2. Do I pressure cook at 15psi?

3. I have 2cc of spore solution, how many pint jars can or should I do?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

Extras: Filter Print Post Top
OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15253634 - 10/20/11 07:03 PM (13 years, 2 months ago)

Bump. Anyone want to give me a hand?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

Extras: Filter Print Post Top
InvisibleViruk
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Registered: 03/12/06
Posts: 230
Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15254757 - 10/20/11 10:49 PM (13 years, 2 months ago)

Your calculations are on the mark!
PC at 15 psi for 15-20 minutes, I do 20 because I'm paranoid.

I've never had success injecting spores into an LC, but... at 2CC I'd dump them all into a 1 cup LC.
Fill up that pint jar half way, you'll also never need to provide fresh air that way.
1 cup is enough for tons of LC's, you get 1 going, you keep it sterile, you can keep it going forever, and with less room you'll have a better chance of the spores meeting up, and something actually happening.

Good luck!

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Viruk]
    #15255030 - 10/21/11 12:07 AM (13 years, 2 months ago)

So one cup water, 9.5 grams and that should be good? Another question, if you haven't had success injecting spores into an LC, what else would you do with one? A little bit confused now..


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

Extras: Filter Print Post Top
OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15256319 - 10/21/11 10:51 AM (13 years, 2 months ago)

So I should use 2cc for one pint jar, filled with one cup water and 9.5 grams turbinado, correct?

I have been using lids with one 1/4" hole with polyfill, and a sheet of tyvek under the ring. Will this work?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

Extras: Filter Print Post Top
Offlineigigi
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15257009 - 10/21/11 01:44 PM (13 years, 2 months ago)

Roger, your calculations are correct, sir.
Your lid tek should be sufficient to keep your LC clean..
The amount of spores used is whatever.. it'll just speed up the process.. but if it's just 2cc left just shoot that load and after the LC stops growing: you've got 236cc of LC, or 23 10cc syringes!

As for "if you haven't have had success injecting spores into an LC, what else would you do with one? A little bit confused now.."
What has been suggested above is that *if*, depending on your sterile procedures :wink:, your LC is viable (got to try it out on a couple of jars before you know).. you can make that LC your "master LC."  Sterile syringe+ 1-2cc from master LC = materials to start yourself another LC.. That way you drain your master much much slower AND you're not constantly sticking needles into the master, increasing your risk of contam'ing it.  :thumbup:

:discorex:


--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic.  Click and READ the first link.  I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/

Edited by igigi (10/21/11 01:47 PM)

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InvisibleViruk
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15260321 - 10/22/11 03:11 AM (13 years, 2 months ago)

Quote:

Dodongo said:
So one cup water, 9.5 grams and that should be good? Another question, if you haven't had success injecting spores into an LC, what else would you do with one? A little bit confused now..




Hopefully this might still come in handy.
I have injected spores into a turbinado LC many times, and haven't had a thing happen, it's very sad, but it doesn't seem to take for me.

Other people have had success, it leaves me very confused myself, but I sadly tried around a dozen times and nothing happened, so I just made a GLC from a WBS jar I had inoculated with 1cc of spores, and injected that GLC into a "master LC". (A GLC if you didn't know is a grain LC, it's basically mycelium water. You take a syringe of sterile distilled water, inject it into a colonized grain jar, shake it up a bit, and suck out the water that now has mycelium strands floating in it)

Anyway,  that master LC was tested, and also injected into the "backup LC"
And finally more jars were inoculated with the GLC, when that runs out, I make more jars from the backup LC (which has also been tested), and then make a new GLC out of that. If the GLC fails, I go to the backup, if that fails, the master, if that fails (GOD FORBID!) I go to spores.

So if your LC works out, make another, and some jars, and take a syringe out of the LC. Inject a cc or 2 of it into the new LC, then the jars, and you'll get your test while you make the backup LC. If the jars come out clean, you know the new LC is probably clean, and so is the old LC.
Then you take all of your new jars from the new LC, and the old LC is stored in the fridge, where it will stay viable for years!

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Viruk]
    #15260410 - 10/22/11 04:22 AM (13 years, 2 months ago)

Yeah, I actually ended up just using my last 2cc to innoc 2 pint jars of WBS prepared very precisely, and dried much better than before. I see no reason why they shouldn't colonize, but who knows. If I have any luck, I plan on first doing a GLC on the better of the two, and then doing g2g on each.

Any idea how many quart jars I can g2g with one pint jar?

On another note, I am never buying Nature's Song WBS again, so much corn that it turned my first jars into goopy messes. Also seems like it has a lot of sunflower seeds but perhaps that's normal, I haven't bought any other brands. It was what they had at the store I was buying supplies at, and I figured a good brand would equal good quality.

Now that this spore syringe is spent, I plan on making another out of a print I got in the mail yesterday. If anyone knows of a very foolproof (I have no glovebox) and easy tek to make syringes, please let me know!

Thanks for all the help, bros.

:ancientaliens:


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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InvisibleViruk
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Posts: 230
Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15264995 - 10/23/11 05:46 AM (13 years, 2 months ago)

Good plan. Wbs is picky at first, but once you figure out how dry it needs to be you won't have any more problems.
Basically if you can shake up the jars after they were pressure cooked, and no clumps stay in the jar, all the grains shake free, then you know it's dry enough.

I also make a GLC then instantly g2g as the extra moisture will be absorbed by the new jars.

I'd say you could comfortably g2g 4-5 quart jars with 1 pint jar.
If you shake it up well afterwards it should take about a week to fully colonize again. The less you use from the pint, the longer the quart jar will take to colonize, and contaminates will therefore have a better chance to get a foothold. If you have no glovebox, I would maybe only do 3-4 to be safe.

On the subject of a glovebox, it really doesn't have to be much. Take a garbage bag or box, cut an eyehole in it, and hang it up somehow, or prop it up. This is really basic, but it will create a shield that prevents a lot of particles from falling onto your work surface. As long as you don't expand the bag and pull in lots of air, or blow air under the box, you will keep relatively sterile. Wear some standard latex gloves, and just shove your hands under the edge.

With a basic glovebox, you can now easily just scrape the spores into a sterile jar (with a sterile knife, or piece of wire) and take a syringe that's already filled with sterile water, flame the needle, then shoot it in there at all the spores to mix it up the solution. Then suck out all the water carefully (air contains contaminates). If you do get air in the syringe you can aim the point out, and eject the air.

Should all work out fine!

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Viruk]
    #15285423 - 10/27/11 10:39 AM (13 years, 2 months ago)

I haven't read anything about having to stir the sugar into the water or not. I shook the jars a little to get it stirring up, but my lids were already on so I didn't want to take them off and actually stir the inside with something.

Will the heat from the PC mix it all up?

Also just an update, I'm making 3 pint jars of LC right now, going to inoculate with 2cc each of cambo MS that I made from a print yesterday.


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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OfflineDodongo
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15298136 - 10/30/11 02:32 AM (13 years, 2 months ago)

Quick question guys, will my first signs of growth be small clear pieces floating around, and mainly on the bottom of the jars? Also the spore syringe I used to innoc had quite a few visible spores in it, it looks like in the LC a lot of them are bunching together, held by sort of clear goop. Reminds me of when you see mosquito eggs in the water, if you know what I'm talking about.


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun

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OfflineSickDog
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Re: Questions about Turbinado LC tek [Re: Dodongo]
    #15298164 - 10/30/11 02:49 AM (13 years, 2 months ago)

1ml h20 = 1g. keep your sugar under 4% and you're good. pc for 15-20min @ 15psi.

myc in LC looks like white gobs of snot floating. i'm sure you can find plenty of pics on this site.

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InvisibleViruk
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Re: Questions about Turbinado LC tek [Re: SickDog]
    #15304971 - 10/31/11 06:07 PM (13 years, 2 months ago)

Shaken mycelium looks like white globs of snot floating around, sorta stringy.
However if you just walk up to a jar that's been sitting a day, you should see a cottony white mass forming on the bottom, that breaks up into pieces when you mix it.

Also your sugar should have dissolved enough, I always really make sure with mine though, and filter it before adding it to the jar.

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Offlinejim617
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Re: Questions about Turbinado LC tek [Re: Viruk]
    #15305453 - 10/31/11 07:35 PM (13 years, 2 months ago)

Raw Sugar Lc is the best imo for Lc, but then I realized how much better it is to just make a grain LC.
60cc syringe, shoot it in , suck it out, done! guaranteed no contams!because you can verify that while its on the grains,cant do that in liquid).


--------------------
MrFunGuy: "I figured if I put a 15lb cinder block on top of the pot lid that it would cook @15 psi."[]

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InvisibleViruk
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Re: Questions about Turbinado LC tek [Re: jim617]
    #15312033 - 11/02/11 03:30 AM (13 years, 2 months ago)

Ya but it doesn't grow in a GLC, squirt that into an LC and you have a lot of backup.

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