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InvisibleTranscendingLife
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TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) * 11
    #15234219 - 10/16/11 03:23 PM (12 years, 4 months ago)

Making a Liquid Culture Using Two Ingredients


In this tek, I'll take you through the process of only using Water+(Extra Light) Malt Extract.
I've had tremendous success with this method of making LCs
This tek works for Edibles as well as Actives.

Link to My Journal

SUPPLIES


-1 lb of DRY (Extra Light) Malt Extract
  - Obtained at the local brewery or brew store.
  - The Lighter the Malt Extract the clearer your LC will be.
      - I use Extra Light Malt Extract, but any Malt Extract will work.
-1 Gallon Distilled water
  - Tap water works fine for this tek, also.
-Digital Scale
  - I don't like the guessing of using tsp., tbs or ccs.
-Pot for Boiling water
-Stirring Spoon for (EL)ME + Water
-Measuring Cup for Measuring Water
-Pressure Cooker
-Liquid Culture "No-Tilt" Jars
  - You can use any of the Lid designs in my tek; I recommend using Whatmans for LCs as to guarantee the filter stays dry.
-Spore, LC or GLC syringe for Inoculation
-Alcohol Lamp for Flame sterilizing the Needle


Measuring, Cooking & Sterilizing


- Measure out 400mL of water per pint jar, or 600mL of water per quart jar.
    ***Pictures of Measurements are for 1 pint jar.

- Pour the water in your pot & start your stove.
  - Heat makes solids dissolve quicker (remember basic Chemistry)
- Prep your PC w/ water & start the burner under it.
- Measure out .667g of (EL)ME per pint, or 1g per quart.

- Once your water in your pot is hot (it doesn't have to be boiling), pour & stir in you (EL)ME
  - My water is normally around 170F when I stir in my ELME.
- Stir in your (EL)ME until it's fully dissolve. NO CHUNKS!
- Pour your hot liquid into your jar & seal w/ the lid.

***This picture is of a .667g jar compared to a 3g jar, notice the color difference:

- Place Tightly sealed jars in the PC

- Put the Lid on your PC w/ the Toggle Weight off.
- Wait until steam starts pouring outta the PC & Pressure rises a bit.
- Put Toggle weight on Lid @ 15 PSI.
- Pressure cook for 30-45 minutes (depending on volume) @ 15 PSI.
- Let cool over night

Inoculation Process


- Standard Sterile Procedure
  - Sterile Procedure:
      a.Turn off Heat/AC, Shut all windows & turn off all Fans.
      b.Oust Room
      c.Shower, wash hands, glove hands & wear a surgical mask.
      d.Oust once more

***Here's the picture from above after PCing of the two different jars just switched around (3g ELME per 600mL water & .667g per 400mL)


- Get all your supplies in order in front of you.
- Flame sterilize your Needle.
- Wipe inoculation/extraction port w/ iso
- Extract LC or GLC from known Clean Culture
- Flame sterilize the Needle.
- Wipe inoculation/extraction port w/ iso
- Inoculate clean LC jar
  - I use 2cc of GLC or LC solution per jar (regardless of size) or 1cc MS spore solution per jar




***Here's an example of how light you can get a good mixture.
Left is 400mL water jar injected w/ 20cc of Clean LC solution. It was a GLC that I needed to extend the life of.
Two Standard measured jars then the heavy ELME jar.


Success w/ this recipe for LCs:
  - ATL & DT, respectively, MS spore inoculation 2 weeks after 1cc injected:


  - PE6 GLC jar 60cc Clean LC solution injected for longer storage & nutrient addition.
***First picture is of the GLC after 2 weeks, Second is 1 week after Clean LC injection:


- PE6 GLC inoculated w/ Clean LC next to standard LC solution (2 weeks after each):


- Enoki Golds:


**Link to my Dancing Tiger Grow Log using the Above LC**

As always with my teks, if I've left something out Please let me know & Feel free to ask Questions.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


Edited by TranscendingLife (04/16/12 09:39 AM)


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InvisibleEvilMushroom666
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15234295 - 10/16/11 03:38 PM (12 years, 4 months ago)

:borat:

Being a convert to the LME LC I have to say great work sharing this gem
with the community.


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Invisibledtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: EvilMushroom666] * 1
    #15234335 - 10/16/11 03:46 PM (12 years, 4 months ago)

Fairly straight forward, looks good.


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Invisiblemenelaus
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: EvilMushroom666] * 1
    #15234342 - 10/16/11 03:48 PM (12 years, 4 months ago)

mmmmmmmmmmm ELME

:homerdrool:


I've seen people recommend dropping the dextrose. Now it's official I gotta try it. Congrats your teks are pure business :thumbup:


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: dtowntoker] * 1
    #15234347 - 10/16/11 03:50 PM (12 years, 4 months ago)

The ratio is 1g ELME per 600mL of water for quarts.

Now if you multiple that times 2/3 (what will fit comfortably in a pint)
You get .667g ELME per 400mL of water for pints.

It's the same ratio.

Menelaus - :thanx: for the praise. Leave out dextrose. It's not necessary IMHO.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleEvilMushroom666
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: menelaus] * 1
    #15234348 - 10/16/11 03:50 PM (12 years, 4 months ago)

Edibles love the mix as well, here is a Shiitake LC made with .6 of a gram
of LME(400ml water). I inoculated the mixture with a few kernels of colonized
grain spawn using my laminar flow hood:



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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: EvilMushroom666] * 1
    #15234438 - 10/16/11 04:08 PM (12 years, 4 months ago)

O yea, forgot to mention that.
I've got over 5 different types of Edibles on this mixture of ELME+water too.

:doh:

Enoki Golds pinning in jar (they're extremely aggressive)


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisibledtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15234458 - 10/16/11 04:12 PM (12 years, 4 months ago)

Quote:

TranscendingLife said:
The ratio is 1g ELME per 600mL of water for quarts.

Now if you multiple that times 2/3 (what will fit comfortably in a pint)
You get .667g ELME per 400mL of water for pints.

It's the same ratio.

Menelaus - :thanx: for the praise. Leave out dextrose. It's not necessary IMHO.




I didn't see the part where you weren't filling the jars up equally, hence the edit


--------------------


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OfflineKaptKid
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15234472 - 10/16/11 04:14 PM (12 years, 4 months ago)

Will have to try this one.
Marked it for future use.

Thanks


--------------------
Child of the 60's, Tripping ever since.


:sun:


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: dtowntoker] * 1
    #15234485 - 10/16/11 04:16 PM (12 years, 4 months ago)

dtowntoker - NP bro.

Kaptkid - :super:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisibleflameclown
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) *DELETED* [Re: KaptKid] * 1
    #15234494 - 10/16/11 04:19 PM (12 years, 4 months ago)

Post deleted by flameclown

Reason for deletion: [this post is damn old]



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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: flameclown] * 1
    #15234628 - 10/16/11 04:45 PM (12 years, 4 months ago)

I don't use a magnetic stirrer.
I just swirl the LC in the sealed jar, which is why I recommend using Whatmans for LCs.
That way if you get a little splash in the jar, you know the filter won't get wet.

The two "inoculation" spots are actually:
1 inoculation port
1 extraction port via SS tubing for a "No-Tilt" Design.
The lid tek is in the tek as well in the "Supplies" Section.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineBargainBab
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15234798 - 10/16/11 05:19 PM (12 years, 4 months ago)

This should be the industry standard in LC, it cuts out magnetic stirrers and is actually up to date with info. Great write up :super:


--------------------
Oh wow, GOOD Nyborg!


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: BargainBab] * 1
    #15238165 - 10/17/11 11:59 AM (12 years, 4 months ago)

BargainBab - :thanx: :bow:

I like to Keep It Simple.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineBom_Tombadil
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15238241 - 10/17/11 12:13 PM (12 years, 4 months ago)

magnetic stirrers are great but they definitely are not necessary the only reason i have one is because i got one for free. sometimes i will take an lc and put the stirrer on real low to constantly agitate the solution and will see a faster colonization rate but i hardly even use lcs any more i just do grain transfers but lc can still come in handy. nice tek i love using malt extract for lc as well as brewing the occasional beer. :thumbup::thumbup:


--------------------
"There is only one Lord of the Ring, only one who can bend it to his will. And he does not share power."


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: Bom_Tombadil] * 1
    #15238288 - 10/17/11 12:24 PM (12 years, 4 months ago)

:kenthumbup:

I do GLCs or LCs for longer storage of cultures & stretching cultures.
I prefer G2G when growing. Messing w/ flaming needles is just too much of  hassle at times.
Especially, when I can G2G 12 jars quicker than inoculate 12 jars w/ a needle.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisibleflameclown
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) *DELETED* [Re: TranscendingLife] * 1
    #15238368 - 10/17/11 12:41 PM (12 years, 4 months ago)

Post deleted by flameclown

Reason for deletion: [this post is damn old]



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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: flameclown] * 1
    #15238381 - 10/17/11 12:44 PM (12 years, 4 months ago)

Very close.
G2G I get full colonization @ 1:10 in 14-17 days. @ 1:5 It's done in 10.
LCs are done around 18-21 days.
Not much difference, really.

I do G2Gs in my SAB. No flowhood yet.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Offlinetripdawg420
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife] * 1
    #15238491 - 10/17/11 01:16 PM (12 years, 4 months ago)

:congrats: wow where the fuck is your TC tag :shrug:


--------------------
HUSTLER
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epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

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http://www.shroomery.org/forums/showflat.php/Number/11525679
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http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineSKrink
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: tripdawg420] * 1
    #15238583 - 10/17/11 01:42 PM (12 years, 4 months ago)

Good job thanks for the tek!

Been meaning to try out your ELME method for awhile now, but my "local" brew shop is quite a drive away. Plus they have really weird opening hours.

Today, I have missed them by a minute for the LAST FUCKING TIME... time to order some malt extract on Amazon or something..


--------------------
:crazy2: SWEET POTATO HOME FRIES :crazy2:
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InvisibleDynGBreeD
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: SKrink]
    #15238591 - 10/17/11 01:43 PM (12 years, 4 months ago)

:nothingtoadd:


--------------------


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: SKrink]
    #15238605 - 10/17/11 01:46 PM (12 years, 4 months ago)

Quote:

SKrink said:
Good job thanks for the tek!

Been meaning to try out your ELME method for awhile now, but my "local" brew shop is quite a drive away. Plus they have really weird opening hours.

Today, I have missed them by a minute for the LAST FUCKING TIME... time to order some malt extract on Amazon or something..




Yea, you can get it for pretty cheap there too. I'm blessed to have a local brewery down the road.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleMunchauzen
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: tripdawg420]
    #15238683 - 10/17/11 02:04 PM (12 years, 4 months ago)

Quote:

tripdawg420 said:
:congrats: wow where the fuck is your TC tag :shrug:




:whathesaid: nearly 10k posts in just one year and always very helpful comments with well researched and documented teks. just look at his journal....


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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: Munchauzen]
    #15238724 - 10/17/11 02:15 PM (12 years, 4 months ago)

Thanks for the votes of confidence, guys.
But no worries. A "TC" tag is just a label.
I'm not that worried about it & know the mods are really busy with other things on the site.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisibleflameclown
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) *DELETED* [Re: TranscendingLife]
    #15238825 - 10/17/11 02:43 PM (12 years, 4 months ago)

Post deleted by flameclown

Reason for deletion: [this post is damn old]



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InvisibleTranscendingLife
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: flameclown]
    #15238845 - 10/17/11 02:47 PM (12 years, 4 months ago)

I don't shake after the G2G has started colonizing the grains.
I figure I beat them up enough.

yea. here's a full sized picture of it (no words though):


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
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"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #15239631 - 10/17/11 05:41 PM (12 years, 4 months ago)

A+ TL :thumbup:

Quality work!

:murray:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: Wing]
    #15241394 - 10/18/11 12:14 AM (12 years, 4 months ago)

Great! Will try out this today.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: extrema]
    #15242024 - 10/18/11 07:03 AM (12 years, 4 months ago)

:winning1:

:towelie:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #15242068 - 10/18/11 07:40 AM (12 years, 4 months ago)

You are the man TL. Thanks for showing me!:thumbup:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: CapSlinger]
    #15242598 - 10/18/11 11:51 AM (12 years, 3 months ago)

:thanx: guys. Hope the tek works as well for you all as it does me.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #15242810 - 10/18/11 12:55 PM (12 years, 3 months ago)

Thanks, Great tek! Love the simplicity of it all.... now if I can just find big gauge long azz needles.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildlifeonmars]
    #15242828 - 10/18/11 01:00 PM (12 years, 3 months ago)

A lot of the sponsors on here sell them.
I get mine locally, also, from the local Farm+feed store. Just ask them where the vet supplies are.
They normally have 1.5" long 18 or 16 gauge needles & a large variety of syringe types (12-60cc).

If you're referring to removing the LC solution, check out my "No-Tilt" design lids for LCs:
https://www.shroomery.org/forums/showflat.php/Number/14186384#14186384


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #15242971 - 10/18/11 01:40 PM (12 years, 3 months ago)

i have used turkey or marinade injector you can usually find them in a grocery store for pretty cheap. Something like this http://www.google.com/products/catalog?q=turkey+injector&hl=en&safe=off&prmd=imvnse&bav=on.2,or.r_gc.r_pw.,cf.osb&biw=1366&bih=532&um=1&ie=UTF-8&tbm=shop&cid=1762985217881343179&sa=X&ei=_dSdToeyL-TgiAKnwtX6CQ&ved=0CGUQ8wIwAA The LC needle from Fungi Perfecti work ok too but they are like $9 a pop and if you have an LC lid with a whatman or similar water proof filter you can just tilt the fucker and use you standard 16 or 18 gauge 1.5 inch needle.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: Bom_Tombadil]
    #15243000 - 10/18/11 01:46 PM (12 years, 3 months ago)

yea, you can tilt the jar, even w/out a "water proof" GE filter.
Just make sure not to get the filter wet.
I like the ease of my "No-Tilt" Design, because I can use both hands in extracting the colonized LC solution.
I hate having to stretch my hand to pull the plunger back when extracting LC solutions.


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"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #15243037 - 10/18/11 01:58 PM (12 years, 3 months ago)

Question...you showed 2 jars and one had colonized for I think 2 weeks and then next to it was a "clean" lc about a week old. Do you start your 1st lc with grain and then draw out of that into another a lc to 'clean' it per say? I just did an lc with some tissue and it`s coming along well but do you have to go from that to another lc??


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: KaptKid]
    #15243049 - 10/18/11 02:02 PM (12 years, 3 months ago)

Quote:

KaptKid said:
Will have to try this one.
Marked it for future use.

Thanks




Ya nice was just looking into this ,nice timing


:slomo:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: BlueIndian]
    #15243076 - 10/18/11 02:10 PM (12 years, 3 months ago)

Quote:

BlueIndian said:
Question...you showed 2 jars and one had colonized for I think 2 weeks and then next to it was a "clean" lc about a week old. Do you start your 1st lc with grain and then draw out of that into another a lc to 'clean' it per say? I just did an lc with some tissue and it`s coming along well but do you have to go from that to another lc??




I was messing with ratios at that point & found the right one, finally.
Just wanted to show some the difference between making a normal LC jar vs. a GLC that I injected 60cc of clean LC into.
When I say clean LC, I mean an LC that has not been inoculated, yet.
It's just water+ELME. That way I can extend the life a bit of my GLCs


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #17844078 - 02/21/13 08:11 PM (10 years, 11 months ago)

I know its an old thread. I'm bumping it anyway with a question rather than start a new one.

TL, it looks like you're results imply that 1 gram of LME would be the "sweet spot" for sugar content. As historically many other threads suggest %4-%6 sugar, I'm wondering if you found those higher concentrations to have a negative affect on mycelium growth? I know that too much can act as a preservative and inhibit growth, but did you find that the traditional measurement cause problems for you?


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildernessjunkie] * 1
    #17844384 - 02/21/13 09:19 PM (10 years, 11 months ago)

I know that we used this recipe b4 then we removed the Dextrose (sugar) from that aspect to lessen the sugar load and had better success :2cents:

http://www.shroomery.org/forums/showflat.php/Number/12830423#12830423

I had one jar of success with sugar based LCs like honey and SitR or karo so using damions recipe I had some success then once I removed most of the sugars (Dex) I had almost 100% success as long as sterile procedure was spot on.  :syringe:

its also why we use grain LCs also, less sugar, clean myc used to inoc LCs and G2G makes alot of spawn :yesnod:

TL prob has more to add but that was the discussion I remember having :blazed:

:getstoned:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17844458 - 02/21/13 09:31 PM (10 years, 11 months ago)

and if I remember correctly TL did several LCs of diff amounts of ELME.  he did several increments above and below his normal amount used. 
I dont think he made a log of it nor do I remember what results came of it, :he might.  :wow:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17844547 - 02/21/13 09:42 PM (10 years, 11 months ago)

So if I understand what you're saying, then the lower LME content works just fine for the mycelium. And as a byproduct has the benefit of minimizing the growth of undesirables? Because of that, a 1% solution is more advantageous than higher concentrations.

Am I off there?

Will %4 have a negative/positive impact on mycelium growth? Assuming sterile, and all else equal?

Thanks for the input.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildernessjunkie]
    #17844682 - 02/21/13 10:06 PM (10 years, 11 months ago)

yeah, but too little will not have enough nutrients to achieve full colonization of your LC.


and no 4% is the recommended sugar volume for almost all LCs.  however germination is not recommended in sugar based LCs.  expanding your live myc supply is the intended use.  but it works for some. :smirk:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17844899 - 02/21/13 10:45 PM (10 years, 11 months ago)

Nice write up TL

beeen thinkin of moving to LC's for my Oysters.

But i love g2g!


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17844933 - 02/21/13 10:57 PM (10 years, 11 months ago)

I don't plan to do any germination. That's just a bad idea on all accounts. I've got clean agar culture to work with.

Would it be safe to say then that, about 1% for initial transfer. Then test for cleanliness. Then put into %4 for bulk usage?

Am I on the right track here? I've got clean agar via this tek:

Prof P's No Pour Agar Tek

Except with the lids made with a SFD cut out disk and SHIP's. The plan is to inject some sterilized LC solution to the agar jar, wait for colonization, then transfer to bulk LC. In this instance would you recommend the %1 or %4? Any issues with this process? Any guidance is welcome.

Thanks again for your time.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildernessjunkie]
    #17845745 - 02/22/13 03:53 AM (10 years, 11 months ago)

no, no issues with that.  lots of people do it that way :thumbup:


but your agar mix is diff than this mix, this thread is for an LC mix, use your agar to work towards a good isolate or to clean up your culture.  then inoculate your LC (via this tek) to use as inoculant for grain jars or do a G2G for more bulk spawn. :smirk:

Im always going to advise the lower sugars over any 4% solution. :yesnod:

:bonghit2:


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Edited by 13shrooms (02/22/13 04:00 AM)


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17846111 - 02/22/13 08:13 AM (10 years, 11 months ago)

I understand.

The agar via that tek is to insure a clean culture. I'm just adding the LC mix as per this thread to the agar to obtain the initial LC. Rather than agar to the LC. I'm trying to keep the whole system sealed basically. Don't want to open up anything if I can prevent it.

I think I'm good now. Thanks for the guidance.


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildernessjunkie]
    #17847511 - 02/22/13 02:17 PM (10 years, 11 months ago)



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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 13shrooms]
    #17848410 - 02/22/13 05:19 PM (10 years, 11 months ago)

That's pretty close to what I intend to do right there. But I'll be going with Flours rather than whole grain, and I'll be making sure the LC is clean by starting with agar. I think we're on the same page now.

:fonz:


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Re: TranscendingLife's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: wildernessjunkie]
    #18465451 - 06/24/13 01:48 PM (10 years, 7 months ago)

Thanks for this nice tek.


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Re: TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: TranscendingLife]
    #18931715 - 10/04/13 03:20 PM (10 years, 4 months ago)

What is Eldm? Ive been having issues with my Lc's had the first few fail and then the next batch made white honey web like growth didnt look right tossed it out. Have malt/dex laying around , pretty much done with honey, thinking about ordering a no tilt jar and the malt jars from mycopath. Have a laminar flow hood prep station coming from ebay tomorrow and switching from multi to iso to change the gamit but other than that im lost as to what is so hard


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Re: TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 1111nightmare]
    #18931734 - 10/04/13 03:24 PM (10 years, 4 months ago)

saw something about 1 gram to 500 - 600 cc of water what is this ratio?


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Re: TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: 1111nightmare]
    #18931746 - 10/04/13 03:28 PM (10 years, 4 months ago)

The ratio is 1 gram of ELME to 500-600mL of water :shrug:

I stand by this tek as my favorite LC tek, even though I rarely use LCs for much of anything except stone producers and some edibles.


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Re: TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME) [Re: FrankHorrigan]
    #18932204 - 10/04/13 05:19 PM (10 years, 4 months ago)

500cc = 500mL = 500cm3


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