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poke smot!
floccinocci floofinator
Registered: 01/08/03
Posts: 5,248
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The All New SporeDram! (tek)
#1521820 - 05/05/03 12:31 PM (20 years, 10 months ago) |
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Just some random stoner rambling I had inside my head, led to ideas for when you run out of syringes.
A nearby farmer's market offers dram vials with autoclavable caps. SWIM was using them to do agar slants, but soon found a convenient use for them.
At $2.00 a dozen, they were PC'ed at 15 psi for 30 minutes, in tall half-pints, six per. Lids were on loose. Bang, it's sterile, then capped tight and bagged.
So later SWIM was preparing spore syringes and realized the syringe was really dark. He used far too much print. Kinda looked like this:
So he took a dram vial and put half of the spore solution into it. Then some sterilized distilled water was sucked into the syringe to make it 10cc again, and some sterilized distilled water from a clean syringe was added to the dram vial to make it a dram (10cc's).
It kinda looked like this:
Looks like a winner. These cheap dram vials hold enough liquid for one syringe. The only risk of contamination is via. the lid and opening, but you should be sticking sterilized needles around in there.
Plus, small amounts of suspension can be taken out. If you make a concentrated master, just make 10 syringes by sucking up 1cc of the liquid in each.
The only question I have about the process is, do spores keep longer in water or dried in a sporeprint? How long do prints keep in that manner?
Of course there are factors such as, whether you are trying to inoculate a bunch of jars or just get some germination on an agar plate. In the case of jars, a larger percentage of viable spores would help colonization times. In the case of agar plates, more spores = more strains, so older spores may not have many viable spores, although the ones that are still good will be able to germinate.
Edited by poke smot! (09/07/20 01:25 PM)
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JeRiKo1
Stranger
Registered: 02/25/03
Posts: 123
Loc: Germany
Last seen: 18 years, 11 months
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Re: The All New SporeDram! (tek) [Re: poke smot!]
#1521907 - 05/05/03 01:14 PM (20 years, 10 months ago) |
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nice idea, especially for shipping spore-solutio, or even mycel solution. gotta investigate where to find these cutties around here (germany).
-------------------- "All statements are true in some sense, false in some sense, meaningless in some sense, true and false in some sense, true and meaningless in some sense, false and meaningless in some sense, and true and false and meaningless in some sense.''
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Oscuro_lobo
Dark Wolf
Registered: 04/15/02
Posts: 813
Loc: it doesn't really matter.
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Re: The All New SporeDram! (tek) [Re: poke smot!]
#1522842 - 05/05/03 06:46 PM (20 years, 10 months ago) |
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Just wanted to say, awesome idea! Any idea where you can get those vials online? I wouldn't mind a couple, but the ones I find on ebay, I don't think their caps are autoclavable. How do you know yours are/were?
And while not sure, I think spores last longer in print form than a liquid suspension. I base this assumption on the fact prints can last YEARS, while syringes are only supposed to be viable for aprx. 6mnths. -ol
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Anonymous
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Re: The All New SporeDram! (tek) [Re: Oscuro_lobo]
#1523018 - 05/05/03 07:39 PM (20 years, 10 months ago) |
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Don't buy from eBay massive rip off!
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Oscuro_lobo
Dark Wolf
Registered: 04/15/02
Posts: 813
Loc: it doesn't really matter.
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Re: The All New SporeDram! (tek) [Re: daba]
#1523028 - 05/05/03 07:42 PM (20 years, 10 months ago) |
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I don't live in the kind of a community that has a farm store :\ so online's the only place I could get some. And ebay is.. i dunno ... first stop at trying to find some. I just don't know which kind to get when looking at businesses online.
-ol
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resin
Ghetto Monster
Registered: 06/12/02
Posts: 2,815
Loc: Ca$hville
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Re: The All New SporeDram! (tek) [Re: Oscuro_lobo]
#1523071 - 05/05/03 08:03 PM (20 years, 10 months ago) |
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Syringes are only good for 6 months?? You serious?? Where did you hear this??
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Anonymous
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Re: The All New SporeDram! (tek) [Re: Oscuro_lobo]
#1523073 - 05/05/03 08:04 PM (20 years, 10 months ago) |
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Quote:
The only question I have about the process is, do spores keep longer in water or dried in a sporeprint? How long do prints keep in that manner?
Spores in water are viable for roughly 1 year. Spore prints are viable for many, many years. (I've had success with prints dated back to 1999 and 1998!) Mycellium syringes are viable for roughly ~6 months.
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Oscuro_lobo
Dark Wolf
Registered: 04/15/02
Posts: 813
Loc: it doesn't really matter.
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Re: The All New SporeDram! (tek) [Re: daba]
#1523220 - 05/05/03 08:56 PM (20 years, 10 months ago) |
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Oh wewps, my mistake!
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Rustik
Where am I?
Registered: 04/17/03
Posts: 289
Last seen: 9 years, 4 months
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Re: The All New SporeDram! (tek) [Re: Oscuro_lobo]
#1523520 - 05/05/03 11:42 PM (20 years, 10 months ago) |
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Just posted my technique for creating spore solution. Hope this helps, it doesn't require any extra materials like the Dram vials, so it's slightly more economical. http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum4&Number=1523529&page=0&view=collapsed&sb=5&o=7&fpart=&vc=
-------------------- The blue... the blue!!!
Edited by Rustik (05/05/03 11:47 PM)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
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Re: The All New SporeDram! (tek) [Re: poke smot!]
#1523719 - 05/06/03 01:58 AM (20 years, 10 months ago) |
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What I do:
Anno?s pint print syringe tek:
Take a pint jar with lid, make a hole in lid, fill with water for as many syringes you want, screw the lid on, put a layer of aluminum foil over the lid and pressure cook for 30 minutes. Let cool down in the pressure cooker, take the jar out, unscrew the lid with the foil still on, scrape as many prints you want in the water, screw the lid on, place the finger where the hole is over the foil, and give it a good shake. Take off the foil and pull the syringes through the hole, you will likely have to tilt the jar to do this.
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poke smot!
floccinocci floofinator
Registered: 01/08/03
Posts: 5,248
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Re: The All New SporeDram! (tek) *DELETED* [Re: Anno]
#1523947 - 05/06/03 07:11 AM (20 years, 10 months ago) |
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Post deleted by poke smot!Reason for deletion: x
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KOPELANDIAA
Stranger
Registered: 11/17/01
Posts: 805
Loc: under a pine
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Re: The All New SporeDram! (tek) [Re: poke smot!]
#1524155 - 05/06/03 10:04 AM (20 years, 10 months ago) |
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yes it's what my friend always did with good success...i
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