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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,886
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Re: EvilMushroom666's Take on BRF Cakes [Re: djmako7]
#20640921 - 09/30/14 04:03 PM (9 years, 3 months ago) |
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Awesome writeup. It's frustrating when people have issues with a writeup that rehashes preexisting methods. The addition of any new information on top of verified information is extremely helpful, as well as new pictures.
Also, the ensuing discussion has been particularly invaluable to me. Thanks again to OP for the excellent guide.
A few questions:
1. "Whatever method you use do not forget that an enclosed area + iso alcohol fumes + flame = BOOM."
So after I wipe down my gloves and instruments with 70% iso, I should leave the door open while inoculating? I've heard inoculating in a closed bathroom after spraying it down is a good method, but since I'll have an open flame, what's the best way?
2. Your box uses cutoff gloves + separate latex gloves instead of full gloves attached to the box. What is the advantage to your method over the latter? Is it simply easier to move your syringe and cakes and instruments in and out of the box or just preference? Edit: This has been answered ITT. He prefers this method because it's easier to use new nitrile gloves through the glove tunnel than it is to keep cleaning permanently attached gloves. It's also beneficial to be able to just twist up the protruding ends of the tunnel and stuff them in the entry points to block off the airflow to prevent contaminants. Also, it makes everything easier to handle.
3. RE: Surgical Mask - Should I also wear a hairnet or hat or something as well if it's advisable to shower and brush my teeth?
4. If my jars get contaminated, is it alright to simply wrap them in a trash bag and toss them in the garbage? Or should I PC/boil them and THEN toss them out? I'm equally concerned for my own home/operations as wherever my trash winds up. I don't want to turn my town into zombies!
5. Once the mycelium has reached 100% and I've allowed a week for it to consolidate, I no longer need the strict sterilization techniques, do I? At this point, is the mycelium strong enough to withstand whatever potential contaminates are in my house where I'll be removing it from the jar, rinsing, dunking and rolling?
6. When sterilizing with boil, is it possible to stack jars on top of one another if separated by something, say additional rings or tin foil barriers?
7. A properly fitting lid with a small hole in the top for the steam to escape is fine, correct?
Thanks again!
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motherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia
Last seen: 8 months, 15 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: stareatclouds]
#20641779 - 09/30/14 07:01 PM (9 years, 3 months ago) |
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Since I just did this tek I figured I might try to answer some of questions. Others please feel free to correct me or add anything I missed.
1. "Whatever method you use do not forget that an enclosed area + iso alcohol fumes + flame = BOOM." As in just be very careful where you are flame sterilising and where you are wiping down with ISO, can use what ever you find is the safest. Leaving the door open would defeat the purpose of still air and I heard bathroom are the areas with the most contaminates. Maybe flame on a different surface you can turn around and do to give you distance.
Personally I use hospital grade disinfectant so I dont have to worry about fumes and open flames and still works all the same, or hopefully.
3. RE: Surgical Mask - Should I also wear a hairnet or hat or something as well if it's advisable to shower and brush my teeth? Its advisable to shower, scrub really well especially your arms and hands then brush your teeth. You dont really need to bother with a mask/hair net because people can inoculate in open air and they still get no contaminants.
4. If my jars get contaminated, is it alright to simply wrap them in a trash bag and toss them in the garbage? Or should I PC/boil them and THEN toss them out? I'm equally concerned for my own home/operations as wherever my trash winds up. I don't want to turn my town into zombies! Yes that would work but I dont like to throw out a new jar so I would boil them for an hour, never opening indoors then dispose in an outdoor bin. Never open indoors and be careful to not get anything on your clothes which you bring back indoors.
5. Once the mycelium has reached 100% and I've allowed a week for it to consolidate, I no longer need the strict sterilization techniques, do I? At this point, is the mycelium strong enough to withstand whatever potential contaminates are in my house where I'll be removing it from the jar, rinsing, dunking and rolling? No you dont, you are dunking in unsterilised water and rolling from verm straight in the bag then putting in a chamber in open air. Not too much worry but dont be dirty either and keep your FC off the ground.
6. When sterilizing with boil, is it possible to stack jars on top of one another if separated by something, say additional rings or tin foil barriers? Yes same as you would for PC so stack all the same.
7. A properly fitting lid with a small hole in the top for the steam to escape is fine, correct? Yes as long as its not a lid of a different size to the pot and keep checking the water because it often boils dry.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,886
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Re: EvilMushroom666's Take on BRF Cakes [Re: motherchimp]
#20642120 - 09/30/14 08:13 PM (9 years, 3 months ago) |
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Thanks a lot for the reply.
The sterilization is the biggest issue I'm having with so far. I'll likely be storing my SGFC in my spare room, but ideally I'd inoculate them in a different part of my house, likely in my kitchen on the table.
I'm assuming I'm overthinking things. I'm only wiping down my gloves and the inside of my glovebox with isopropyl alcohol, right? So holding the needle over flame outside of the glove box should be fine as long as the isopropyl alcohol on my gloves has dried?
I wish there was a video demonstrating the entire sterilization aspect of this part of PF Tek.
Thanks again!
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motherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia
Last seen: 8 months, 15 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: stareatclouds]
#20642265 - 09/30/14 08:49 PM (9 years, 3 months ago) |
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Well there are many videos, firstly: http://www.mushroomvideos.com/BRF-Pf-Tek and theres just so so many on youtube
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,886
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Re: EvilMushroom666's Take on BRF Cakes [Re: motherchimp]
#20642483 - 09/30/14 09:38 PM (9 years, 3 months ago) |
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Yes, I've seen RR's videos, but he uses a different setup than most, and he's also in a pretty big room so he can film properly. I've seen other videos where people spray down rooms with bleach (which isn't advisable since bleach is a surface disinfectant), different videos that take place in a bathroom, pictures of grow ops in bedrooms and on desks, etc.
I guess I meant videos/better pictures which show the whole process in each PF Tek. When I perfect mine, I'll try and make one.
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motherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia
Last seen: 8 months, 15 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: stareatclouds]
#20642503 - 09/30/14 09:44 PM (9 years, 3 months ago) |
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You could do PF tek in a dirty toilet stall in open air and still get 100% colonisation I would imagine. Im not going to be the first to do it though. Dont take too much time considering what room or space to do it in as long as you wipe down your SAB, close all windows and such, flame your needle and wear gloves you'll be golden.
This tek has some good photos or if you want more theres: http://www.shroomery.org/10256/PF-Tek-v2 and: http://www.shroomery.org/8410/Simple-Cubensis-Growing-Technique
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,886
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Re: EvilMushroom666's Take on BRF Cakes [Re: motherchimp]
#20644454 - 10/01/14 02:06 PM (9 years, 3 months ago) |
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Thanks a lot for the links. I'll be sure to wipe down everything I use, cover myself up, and flame sterilize my needles.
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ClosetMonster
No Fear


Registered: 10/13/14
Posts: 238
Last seen: 9 years, 3 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: stareatclouds]
#20704792 - 10/15/14 12:48 AM (9 years, 3 months ago) |
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best write up ... thanks
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Acquario
Experience is Wisdom


Registered: 11/09/14
Posts: 811
Loc: EU
Last seen: 10 months, 27 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: ClosetMonster]
#20816025 - 11/09/14 07:40 PM (9 years, 2 months ago) |
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This write-up was a big help in my beginning stages; thank you very much EM666!
  
Edit for the purpose of asking a question.
I have noticed (at different times of sterilizing jars) that sometimes after they cool, the inside of the jar looks a bit sweaty and other times not so much. Has anyone else experienced this? Is this usual or unusual? I did make the mixes in the very same way and water was not under the aluminum foil, so I am a bit curious..
Edited by Acquario (11/10/14 04:30 PM)
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greencrush420



Registered: 04/14/13
Posts: 1,014
Loc: U.S.A
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Posting so I can come back to this thread later.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: EvilMushroom666's Take on BRF Cakes [Re: greencrush420]
#20913037 - 12/01/14 04:01 PM (9 years, 1 month ago) |
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Glad to see people are still getting use from my write up
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Acquario
Experience is Wisdom


Registered: 11/09/14
Posts: 811
Loc: EU
Last seen: 10 months, 27 days
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Quote:
EvilMushroom666 said: Glad to see people are still getting use from my write up 
No offense to anyone who has contributed to the community by doing a BRF TEK write-up, but this was simply the best to follow when I began
--------------------
Amateur Mycologists United [AMU Q&A Thread] - Ask any question here!
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slushie9090

Registered: 08/14/01
Posts: 2,000
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Re: EvilMushroom666's Take on BRF Cakes *DELETED* [Re: Acquario]
#20913468 - 12/01/14 05:35 PM (9 years, 1 month ago) |
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Post deleted by Rin
Reason for deletion: .
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onewingman
Stranger
Registered: 11/24/13
Posts: 2
Last seen: 6 years, 5 months
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Re: EvilMushroom666's Take on BRF Cakes [Re: slushie9090]
#20992429 - 12/17/14 11:56 PM (9 years, 1 month ago) |
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hey, been following for quite awhile...not real chatty...BUT,I ran into something fun...: I missed, as in mis-placed 2 1/2 pt , inoculated jars...4/1!...they're not contamed and one fruited...and the offspring then dried a GORGEOUS, STEELY BLUE-GRAY... So, I "think" these both and the fruited one, in particular, are kick-ass strong strains... So, my nave level of experience, wonders how to best deal with these two most durable strains...? Any and all useful help, would be gratefully, appreciated! Thanks, onewingman
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jlsmelser
Stranger


Registered: 12/22/14
Posts: 33
Last seen: 1 year, 21 days
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This may be a couple years old. But I have followed it to a tee. Thank You so much for the step by step,pics and text, a lot of hard work! So I say Thank You from a noob giving it his first go. This was everything in one place, great work, Thanks again!
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: EvilMushroom666's Take on BRF Cakes [Re: jlsmelser]
#21127140 - 01/15/15 09:55 PM (9 years, 14 days ago) |
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Glad you had success, glad to see that even though I have been out of the mushroom hobby for years the information I have shared is helping others!
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grandmasshroomery
Tryptonaut


Registered: 01/14/15
Posts: 24
Last seen: 8 years, 3 months
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Just posting to say how helpful this guide has been to me. Currently have 24 1/2 pint wide mouth jars colonizing! Thank you Evil!
-------------------- On my first grow! Thank you for all your help!                          
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jordand77
Newbie


Registered: 02/09/15
Posts: 38
Last seen: 7 months, 22 days
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Hey Evil and everyone else, i'm going to do my first ever grow so i've been reading this guide a lot of times.
There's something i'd like to ask, what can i use instead of 70% ISO alcohol?
The problem is that i live in eastern europe, and believe me i searched a lot for this but i just can't find it. Nor can i find bleach or anything similar.
Will hydrogen peroxyde work well for cleaning the work area ( for Inoculation, i mean the top and sides of the jars and the table)? After some searching on the forum i only found out that it will work to mix with water and mist it in the air.
What other choices do i have? Vodka and 96% pure alcohol ( yes i can find that in our pharmacies)
Thanks.
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kender
The Mushroom Juggler

Registered: 10/30/11
Posts: 21
Last seen: 8 years, 7 months
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Re: EvilMushroom666's Take on BRF Cakes [Re: jordand77]
#21492308 - 04/02/15 01:05 PM (8 years, 9 months ago) |
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First off, commendable fantastic writeup so I don't fear bumping it. EM666 is something of a shiitake mentor of mine (although, he doesn't even know it ) and all of his work should carry on!
Secondly, here's what jordand77 can do even though he's probably found a solution by now, but perhaps this will help others.
Quote:
jordand77 said:
There's something i'd like to ask, what can i use instead of 70% ISO alcohol?
What other choices do i have? Vodka and 96% pure alcohol ( yes i can find that in our pharmacies)
Get the 96% alcohol and some distilled/purified water (tap water will probably be fine and get you close enough as well but distilled is preferred). Then dilute the alcohol! 
Vi x Ci = Vf x Cf
where
Vi is the initial volume Ci is the initial concentration
Vf is the final volume Cf is the final concentration
For example, if you have 500ml of 96% alcohol and you want 70%, just pour some water in. How much water? Plug in the numbers and see:
Vi = 500 Ci = 96
Vf = final volume Cf = 70
So, (500 x 96)/70 = final volume = 685.7
Therefore, add 185.7 ml of distilled water to your 500 ml of 96% alcohol and you will have 685.7 ml of 70% alcohol.
Hope that helps.
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jordand77
Newbie


Registered: 02/09/15
Posts: 38
Last seen: 7 months, 22 days
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Re: EvilMushroom666's Take on BRF Cakes [Re: kender]
#21492789 - 04/02/15 03:10 PM (8 years, 9 months ago) |
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Thanks ) i did use the same formula for this, and it did work pretty well, 15 jars, no contams, some already fruited
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