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Invisiblemycelial
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Registered: 05/07/11
Posts: 85
Need help viewing mycelium/soil microbes
    #15215429 - 10/12/11 06:04 PM (12 years, 7 months ago)

Hey! :laugh:

Just wondering if someone could tell me their procedure for preparing a slide with methylene blue and KOH.

I was attempting to set up some views of compost tea and compost today (live on a farm) and after two hours I was ready to scream. I. Just. Want. To. See. Bacteria. I was applying the tea to a slide, heat fixing, and using a rapid differential staining kit, then viewing on 100x with immersion oil with very little success due to all the artifacts in the way. Ive read you can use KOH to remove these, but how??

Thanks for any advice!


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Invisiblemycelial
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Registered: 05/07/11
Posts: 85
Re: Need help viewing mycelium/soil microbes [Re: mycelial]
    #15216466 - 10/12/11 09:37 PM (12 years, 7 months ago)

Phew! I was on the verge of tears with frustration, but its all cuz my lenses were misaligned lol... :laugh:

So.. never mind!


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OfflineIdiot
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Registered: 11/27/05
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Re: Need help viewing mycelium/soil microbes [Re: mycelial]
    #15217015 - 10/12/11 11:27 PM (12 years, 7 months ago)

Maybe the lenses is misaligned.


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