Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co Certified Organic All-In-One Grow Bags

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinecutemushie
Mushrooms are fascinating.
 User Gallery


Registered: 05/31/08
Posts: 130
Loc: Aus
Last seen: 1 year, 6 months
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: cutemushie]
    #9355788 - 12/03/08 12:49 AM (16 years, 1 month ago)

Just to report back on findings so far.  Using grains with this method sucks.  Grains too prone to contamination.  Also it is hard to control their moisture content.  They tend to dry out too fast.  Also the grains clump together as the mycelium intertwine, making it hard to shake and loosen the mycelium in water.  Giving up grain for this purpose.  Have to look for other methods.  RR recommends culture slants with tongue depressor.  Wonder what would happen if we let the agar and culture dries and resurrect later.  Theorectically should work but not sure if the resurrected mycelium would produce mushrooms normally ie not lose vigor or potential to produce mushrooms.

Cheers,
Cutemushie


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.

Edited by cutemushie (12/03/08 12:51 AM)

Extras: Filter Print Post Top
InvisibleShroominit
Part Time Mycologist


Folding@home Statistics
Registered: 11/29/08
Posts: 4,662
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: cutemushie]
    #9355951 - 12/03/08 01:55 AM (16 years, 1 month ago)

I could have saved you the trouble with the grains and told you that. Refrigerated slants would probably be your best bet. Though I haven't done it, when myc colonizes the wood, it takes a LONG time to decompose/absorb nutrients/whatever else it does.. and this is what buys you extra time to store it.

Extras: Filter Print Post Top
Invisibleroquet
Expat tippler
Male User Gallery


Registered: 05/29/07
Posts: 1,200
Loc: Dubai بجدية عربي...
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: Shroominit]
    #9355996 - 12/03/08 02:20 AM (16 years, 1 month ago)

What's wrong with myc water? You could also dry it out and rehydrate as and when. Though why not leave it as a liquid?

Extras: Filter Print Post Top
Offlinecutemushie
Mushrooms are fascinating.
 User Gallery


Registered: 05/31/08
Posts: 130
Loc: Aus
Last seen: 1 year, 6 months
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: roquet]
    #9363635 - 12/04/08 03:44 AM (16 years, 1 month ago)

Liquid is messy.  Can splash around and invite contamination.  Also if using nutrient liquid, mycelium continues to grow and hence senescence and waste metabolites will build up.


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.

Extras: Filter Print Post Top
Invisibleroquet
Expat tippler
Male User Gallery


Registered: 05/29/07
Posts: 1,200
Loc: Dubai بجدية عربي...
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: cutemushie]
    #9363663 - 12/04/08 04:08 AM (16 years, 1 month ago)

The whole point about myc water as opposed to LC is there's no nutrient in it.

If you used a silicone injection ports with no filter there's no entry point for contams. Splashing around doesn't matter because it's airtight.

Extras: Filter Print Post Top
Invisiblebillyboy36
Stranger

Registered: 05/04/08
Posts: 352
Loc: Canada
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: cutemushie]
    #9365019 - 12/04/08 11:13 AM (16 years, 1 month ago)

Myc water is awesome.

Quote:

Grains too prone to contamination.




You need to work on your sterile procedure if this is happening.  Jar lids etc.

Quote:

They tend to dry out too fast.  Also the grains clump together as the mycelium intertwine, making it hard to shake and loosen the mycelium in water.




What grain did you use?  If you are using this at a small scale, use popcorn.  the kernels are large so they hold a lot of water and break apart easily when shaken while colonizing fast to reduce the time they can dry out.  You'll loose a little bit of myc production with them being that they are so big, but it will eliminate those problems.  also, if they are drying out too fast, find a way to restrict airflow into the jar a little more.

I'd just hate to see you give up on something that easily produces a readily usable myc slurry.

Extras: Filter Print Post Top
OfflineBrennus
Student of Life
Male User Gallery


Registered: 05/31/08
Posts: 3,297
Loc: SE United States Flag
Last seen: 6 years, 10 months
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: billyboy36]
    #9365047 - 12/04/08 11:19 AM (16 years, 1 month ago)

You can't really seal a container airtight for mycelium water for long-term storage - you need some sort of gas exchange or the mycelium will eventually die out. Even master culture slants, which are kept refrigerated and can be stored for 2+ years require gas exchange via a layer of parafilm around the top of the vacutainer.

I think monstermitch has some pictures of really awesome LC jars that would probably be perfect for storing mycelium water long-term; he uses Whatman syringe filters for gas exchange and a vacutainer top for a self-healing injection port.

Extras: Filter Print Post Top
Invisibleroquet
Expat tippler
Male User Gallery


Registered: 05/29/07
Posts: 1,200
Loc: Dubai بجدية عربي...
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: Brennus]
    #9368357 - 12/04/08 08:18 PM (16 years, 1 month ago)

Quote:

Brennus said:
You can't really seal a container airtight for mycelium water for long-term storage - you need some sort of gas exchange or the mycelium will eventually die out.



Where do you get that info from? I've used 9 month old LC in airtight jars no problem. Not sure if the myc was dead or alive but it grew fine when added to grain.

You can even rehydrate a dry piece of tissue and grow from it. I assume in that case the myc is "dead" and then re-animated by water and nutrients.

Extras: Filter Print Post Top
Offlinecutemushie
Mushrooms are fascinating.
 User Gallery


Registered: 05/31/08
Posts: 130
Loc: Aus
Last seen: 1 year, 6 months
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: roquet]
    #9369933 - 12/05/08 12:23 AM (16 years, 1 month ago)

I remember I saw somewhere that RR said they need gas exchange and culture slant cannot be sealed tight.  He was saying to someone to poke holes in vacutainers' caps  I think they may last a few months but longer than that, the oxygen would be used up and they die.  I suppose if the vol of liquid is large enough there may be enough oxgen in there for longer periods.  If you dry mycelium water, the mycelium might stick to the container and it will be hard to retrieve them.

I used wheat grain and it is hard to control the water content.  With regards to contamination, they seemed sterile for many months, and all of a sudden, green mould began to grow or wet spots start to form.  I didn't even touch it or open it.  They were wrapped in cling wrap too, and the contam can start near the bottom.  I think popcorn might work better but still all seed base substrates are a pain to prepare.  I like something really easy, just like cooking instant noodles or making instant coffee.  I think as long as you shake and the seeds touched the micropore tape or tyvek, the likelihood of contamination increases by a lot. 

Another important issue that I think needs to be debated on more is whether mycelium stored dried over a long period of time would lose vigour, just like seeds do.  Often seeds stored for long periods, if they then germinate, tend to produce weak seedlings which easily die off later.  Even if it reanimates and grows again (even vigorously), maybe there might be a reduction in the fruiting potential?

And no I'm not giving up yet.  Just thinking of alternatives.

cutemushie


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.

Edited by cutemushie (12/05/08 12:29 AM)

Extras: Filter Print Post Top
InvisibleViruk
Stranger
Registered: 03/12/06
Posts: 230
Re: Another long term culture storage method? grain, resurrection, mycelium water [Re: cutemushie] * 1
    #15206812 - 10/10/11 06:28 PM (13 years, 3 months ago)

So in the hunt for info about this, I kept coming back to this thread, so I figure this is the best place for this info, even though this place has been quiet for a few years.

Anyway, I had one isolate I was using start to fizzle out. Well I want it back dammit! So going through the jars box one day, I find a few 6 month old Amazon jars, and an equally old Ban Hua Thanon jar. These had all been used for g2g transfers. Now 99% of the WBS I used in it had of course been transferred, but a few grains had stuck behind, fused to the glass with mycelium intact, recolonized a bit, and dried out. So it's sterile enough, I figure, and dump some fresh WBS jars back into the g2g jars to revive them. It worked perfect.

So knock up a grain jar, grain to grain it sloppily, and set a jar aside. It will dry out, and can be reused at least 6 months later.
I think I might try for a year next.
Hopefully this helps you in search of your TEK.
Also there was only ever about a thimble full of WBS left inside the jars, half litre/quart ones.

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co Certified Organic All-In-One Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* long term spore storage GestaltAssault 10,473 3 07/09/03 02:41 PM
by Psilocybin_monkey
* grain to grain transfers ? toreachnirvana 1,416 10 05/11/03 10:05 AM
by micro
* pressure cooking long grain brown rice whitepolkadots 4,420 3 08/14/03 09:33 PM
by Psilocybin_monkey
* The ease of rye grain. Anonymous 14,727 19 06/03/16 03:10 PM
by Disco Shiny
* storage of prints XAZIA 5,104 11 01/25/05 12:20 PM
by PlaCoFuGostei
* Has anyone here had success casing rye grain? EPerson100 34,228 13 02/11/17 06:47 PM
by CubeSteakPoo
* Old Timer's method Dvoid 3,625 12 05/09/01 09:33 PM
by Hippie3
* WET GRAINS! Anonymous 5,250 9 10/21/03 05:52 AM
by shirley knott

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
7,059 topic views. 27 members, 111 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 13 queries.