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OfflineXeR0
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Re: Mushrooms not potent [Re: SteezMe]
    #15194370 - 10/08/11 12:50 AM (13 years, 3 months ago)

Quote:

SteezMe said:
Well i am not going to argue, drugs affect people in different ways.. I have eaten very potent acid and never gotten so much as squiglies in my eyes.
My father who was around when acid was actually ACID and was an every other day tripper and has very strong visual, auditory and sensory experiences with, has been fed more than 3/4 of an ounce of PF's that we all ate just 8ths of and tripped like mad men, him on the other hand had no effects whatsoever. You can disagree with that all you want but one day you will meet someone that you can just burn mushies into and wont feel a thing. Me and him actually have a bet going hahaha when i feed him some that make him trip he is going to owe me 500 bucks. But this bet has been going on for the last 8 years and still... No results, not even a head change



No man, I believe you. I have a friend of mine who's EXTREMELY TOLERANT of pain killers and tranquilizers. He's borderline schizo and he's got bipolar coupled with other shit. Pain killers that would put a normal man to sleep for days would barely have an effect on him. Again though, he's an exception...and only one of few in this world.

In nuclear chemistry, they say that if you expose a population of people to lethal doses of radiation, half of those people would not be affected whatsoever. Neither would their descendants. It's a phenomena scientists still don't understand. That's like saying that if you put 100 people in an oven at 4000C, 50 people would walk out alive and unscathed....WTF?

So again, I do believe you. And I do know these weird things CAN happen as I'm friends with one of those kinds of people. But still, those cases are few and far in between and the chances that my 4 friends had this freakish tolerance is astronomically slim.


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

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OfflineXeR0
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Re: Mushrooms not potent [Re: dtowntoker]
    #15194378 - 10/08/11 12:51 AM (13 years, 3 months ago)

Quote:

dtowntoker said:
You don't want a single spore's mycelium, you want a mating of two spores' mycelium.  Each spore contains different genetics.  Agar is to help you get clean cultures of good growth.



Guess I better start learning how to grow mycelium cultures on agar then....I'm up for that!


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

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Invisibleprismism
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Re: Mushrooms not potent [Re: XeR0]
    #15194405 - 10/08/11 01:02 AM (13 years, 3 months ago)

You probably just got some shiesty spores. My only other advice would be to give Penis Envy a shot. Or even better, an active Panaeolus species. Your friends will not be complaining.


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OfflineWing
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Re: Mushrooms not potent [Re: XeR0]
    #15194413 - 10/08/11 01:05 AM (13 years, 3 months ago)

Quote:

BACK TO TOPIC: I'm still unfamiliar with the concept of MS (which I'm guessing means MultiSpore). How exactly is a syringe of the spores of one species of mushroom a multi-spore? Aren't they the same strain? And if you mean multi-spore as in multiple spores of the same strain, then how exactly do you isolate a SINGLE spore in an agar solution? I'm probably not making any sense so if anyone would care to shed some light on this issue, I'm sure all noobs to mycology would appreciate it.

I'm actually enjoying the opportunity to learn this. This was frustrating at first but now I'm glad to have this run in with the unexpectedness of science. TEACH ME TEACH ME!!!




Yep MS means multi-spore. Basically if you collect spores from one mushroom you get a bunch of spores that have all kinds of different traits.

If you were to put a drop of spores in a petri dish on some kind of growing medium, you would have a bunch of spores mate and the mycelium that grew would be full of tons of genetic traits. It takes two spores to complete a mating and for mycelium to grow. So when there are a bunch of spores finding mated and growing, you can imagine the diverse genetics that could happen. So on a petri dish, the mycelium grows outward from the point the spores germinate from. You would then take samples from several points on the leading outside edge of the growth. For instance 1'oclock, 2, 3, 4, etc, and then transfer those samples into each of their won petri dish.

After they grew for a few days, you would repeat the process for each plate. After a few transfers you'll start to see some real nice rhizmorphic or "ropey" growth and you begin to see sectors. This is when some real progress is made. Again you transfer mycelium from the leading edge of each sector until the mycelium doesn't show any more sectors. At that point you know you have an isolate of a set limited genes.

You would then test each isolate by growing it and see what each iso is. Some may be dark or light. Some may look like a pointy wizards hat (lol). Some may be potent or weak ... or even bunk. Some by not fruit at all! But when you grow out an iso its guaranteed that all your fruits will be very similar and you don't have to worry about the varying genetics.

EDIT: That's a "in a nutshell" explanation.


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Edited by Wing (10/08/11 01:06 AM)

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OfflineXeR0
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Re: Mushrooms not potent [Re: prismism]
    #15195066 - 10/08/11 08:23 AM (13 years, 3 months ago)

Quote:

prismism said:
You probably just got some shiesty spores. My only other advice would be to give Penis Envy a shot. Or even better, an active Panaeolus species. Your friends will not be complaining.



So, some species of mushroom have better yields of potency than others?

winged_1: Thank you for your informative post. I'm actually very interested in trying to cultivate mushrooms via agar isolation. It sounds very interesting. If anything, that's probably the first steps to genetic engineering...:laugh: That could take a lot of time though so I'll have to see how I'll incorporate it into my schedule.

BTW: Is the process of cultivating the Panaeolus species the same for Cubensis? Is it easier/harder to grow? Are they more tolerant/vulnerable to contamination? And, are they definitely very potent? I'd like to read more about that species....


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

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OfflineXeR0
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Re: Mushrooms not potent [Re: XeR0]
    #15195116 - 10/08/11 08:46 AM (13 years, 3 months ago)

Is it also possible that when I put the dried mushrooms in a ziplock that was slightly opened in a sealed jar of desiccant, they lose their potency because it wasn't air/vacuum sealed? I didn't know this before but I stumbled on some info that says exposure to oxygen/air makes the mushrooms lose their potency. If that's the case, then maybe that is to blame and not genetics.


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

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OfflineWing
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Re: Mushrooms not potent [Re: XeR0]
    #15195192 - 10/08/11 09:10 AM (13 years, 3 months ago)

Pans are harder but more potent. I wouldn't say they are more or less prone to contam. You need manure in your substrate and they need to be cased.

I keep my dried mushooms bagged in separate doses within my desiccant chamber with the bags open and see no ill effects.


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OfflineXeR0
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Re: Mushrooms not potent [Re: Wing]
    #15195203 - 10/08/11 09:14 AM (13 years, 3 months ago)

Quote:

winged_1 said:
Pans are harder but more potent. I wouldn't say they are more or less prone to contam. You need manure in your substrate and they need to be cased.

I keep my dried mushooms bagged in separate doses within my desiccant chamber with the bags open and see no ill effects.



That's good to know. Let's me know I'm not doing anything wrong. Also, I've always wanted to know: What do you call growing mushrooms on those trays as in this picture http://en.wikipedia.org/wiki/Panaeolus_cyanescens ?

I want to know how to grow mushrooms like that instead of dealing with cakes.


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OfflineWing
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Re: Mushrooms not potent [Re: XeR0]
    #15195232 - 10/08/11 09:21 AM (13 years, 3 months ago)

I would call that a tray. IMO if you are going to go through the process of making a bulk substrate then you might as well make a monotub unless space is an issue. If you arent familiar with a monotub then check out this link:
How TL does it


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OfflineKevin_X2
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Re: Mushrooms not potent [Re: XeR0]
    #15195388 - 10/08/11 10:22 AM (13 years, 3 months ago)

try them yourself...


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OfflineKevin_X2
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Re: Mushrooms not potent [Re: XeR0]
    #15195397 - 10/08/11 10:25 AM (13 years, 3 months ago)

maaan u sound kinda pissed ahaha, chill out. people are giving you good advice, just take it. no need to argue...


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InvisibleLeopardMan
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Re: Mushrooms not potent [Re: XeR0]
    #15195430 - 10/08/11 10:36 AM (13 years, 3 months ago)

Quote:

XeR0 said:
An eighth should've had them tripping balls




5 grams is the way to go with cubes (RR says that he takes 7 grams). 3.5 is a shot in the dark: Sometimes you have a strong trip but most of the times you just end up watching a movie. And when you can watch a movie, you are not tripping :nono:


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InvisibleViking14
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Re: Mushrooms not potent [Re: Kevin_X2]
    #15197504 - 10/08/11 07:27 PM (13 years, 3 months ago)

Quote:

Kevin_X2 said:
maaan u sound kinda pissed ahaha, chill out. people are giving you good advice, just take it. no need to argue...




Lol, I like how instead of arguing with me, I just got ignored. Then everyone just resaid what I said and I still got ignored. :laugh: Haha, it's fun being the new guy.  :laugh2:

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OfflineXeR0
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Re: Mushrooms not potent [Re: Viking14]
    #15197922 - 10/08/11 08:52 PM (13 years, 3 months ago)

Quote:

Kevin_X2 said:
maaan u sound kinda pissed ahaha, chill out. people are giving you good advice, just take it. no need to argue...



Quote:

Viking14 said:
Lol, I like how instead of arguing with me, I just got ignored. Then everyone just resaid what I said and I still got ignored. :laugh: Haha, it's fun being the new guy.  :laugh2:



I'm not arguing with anybody. All of your replies were helpful and relevant and I thank you all for that. The fact that you took the time to visit my thread, listen to what I'm complaining about, AND reply sincerely is a sign that you're all willing to help...and I'm grateful for that.

Viking14: Yes, you're reply was actually quite helpful. I just checked it again and found that you edited it. I didn't notice that before.

Quote:

LeopardMan said:
5 grams is the way to go with cubes (RR says that he takes 7 grams). 3.5 is a shot in the dark: Sometimes you have a strong trip but most of the times you just end up watching a movie. And when you can watch a movie, you are not tripping :nono:



I'll probably take 5 grams sometime soon to see how that will do. If the trip is up to par with the dosage, then I know I have to recalibrate my mental scale on how many grams of cubes one should take for a given trip level.

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OfflineStusi
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Re: Mushrooms not potent [Re: XeR0]
    #15198160 - 10/08/11 09:58 PM (13 years, 3 months ago)

Did you split 3.5 grams between three people or give each person 3.5 g?  Also, i have noticed with MS cubes it's usually not mind blowing.  I can easily go through 4 or 5 grams and still handle fine.  If you wanna get fucked up take more or get PE or try other species that are known to be stronger (mostly coprophilics).


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OfflineBig Pappa
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Re: Mushrooms not potent [Re: Stusi] * 1
    #15198379 - 10/08/11 11:12 PM (13 years, 3 months ago)

Multi-Spore genetics are like newborn twins...  They are birthed from the same "mother" at the same time...  but given time to grow they both develop there own individual traits, looks and feelings.

Isolating on agar allows you to select "good" sectors of agar grown mycelium that have certain traits, looks and feelings.  It is impossible of course to isolate a single spore and detrimental to do so. 

So like one of the above posters stated, you need to start with spores on an agar plate, let that germinate and grow out.  Then transfer different sectors of that very plate to like 10 more plates and let them grow out.  Some will even transfer a third time although I dont. 

What you end up with is 10 (or more) different agar plates, all with the same species/strain but each with its own characteristics.  Take a wedge from each plate and transfer it to its own grain jar, let that jar colonize and fruit it. 

Do this with ALL your plates individually so you will know what each individual plate will produce.  Pick the best one and you have your isolated strain.  Place a wedge of it in a culture slant and save it for future use.

Hope this helps, and I am no expert by any means, but I do work with agar on occasion :smile:


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Offlinekcbennie
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Re: Mushrooms not potent [Re: Big Pappa]
    #15198479 - 10/08/11 11:48 PM (13 years, 3 months ago)

It sounds to me that you are trying to say that 2+2=4, no matter what, but that is just not the case with hallucinogenics. "Tripping" is not one specific set of experiences.  It can be anything for anybody.  Just because you don't "see" things with your eyes doesn't mean that you didn't see anything. Maybe you focus on a visual experience has caused you to miss something else that could be far more mind blowing than you could ever perceive with your eyes. Ya dig?

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Offlineely2121
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Re: Mushrooms not potent [Re: LeopardMan]
    #15198930 - 10/09/11 04:07 AM (13 years, 3 months ago)






5 grams is the way to go with cubes (RR says that he takes 7 grams). 3.5 is a shot in the dark: Sometimes you have a strong trip but most of the times you just end up watching a movie. And when you can watch a movie, you are not tripping :nono:




:whathesaid:

5 grams and no worries no hurries...and music  :gimmebass:

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OfflineXeR0
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Re: Mushrooms not potent [Re: Stusi]
    #15199687 - 10/09/11 10:24 AM (13 years, 3 months ago)

Quote:

Stusi said:
Did you split 3.5 grams between three people or give each person 3.5 g?  Also, i have noticed with MS cubes it's usually not mind blowing.  I can easily go through 4 or 5 grams and still handle fine.  If you wanna get fucked up take more or get PE or try other species that are known to be stronger (mostly coprophilics).



Of course. I gave each person 3.5g. I'm thinking of getting both Penis Envy strain and the Panaeolus cyanescens. Would like to try those.

Quote:

kcbennie said:
It sounds to me that you are trying to say that 2+2=4, no matter what, but that is just not the case with hallucinogenics. "Tripping" is not one specific set of experiences.  It can be anything for anybody.  Just because you don't "see" things with your eyes doesn't mean that you didn't see anything. Maybe you focus on a visual experience has caused you to miss something else that could be far more mind blowing than you could ever perceive with your eyes. Ya dig?



I agree to an extent. Don't forget that science still operates even when one is tripping. 2+2 WILL ALWAYS EQUAL 4...either tripping or sober. I agree that experiences are very subjective. But a certain dosage of something should've yielded a certain result. If psychedelics were purely subjective, people would be "seeing shit" on very low doses (if they willed it to) just as people would only get buzz effects on high doses (if they willed it to). Dosages would not be reliable then.

My point is that we rely on dosages for a reason. The main culprit here is the amount of psilocybin in the mushrooms...not the amount of mushrooms they took. It's all good though. We've finished that discussion a couple of posts ago...:smirk:


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Drug Experience: Caffeine, Codeine/Morphine, Psilocybin/Psilocin, Dream Herb (Calea Zacatechichi), Melatonin, N,N-DMT, LSD, Pramiracetam, Piracetam, Cannabis

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Offlinek00laid
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Re: Mushrooms not potent [Re: XeR0]
    #15199694 - 10/09/11 10:28 AM (13 years, 3 months ago)

Quote:

XeR0 said:
2+2 WILL ALWAYS EQUAL 4...either tripping or sober.




hmmm, im not so sure.

did you know that .99999(repeating) = 1


and electrons can take the form of a particle AND a wave.

hmmmmm, this reality seems not so consistent. :hehehe:


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