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smily
lookin 4 my ass wit both handz



Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 12 days, 10 hours
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LC Question update EvilmuSh666 mini LID TEK W/PICS
#15193034 - 10/07/11 06:47 PM (13 years, 3 months ago) |
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so while back got some real nice syringes and most all are coming to the last CC.
had pretty good or shall i say great luck with them.
so i guess im looking for a hand hold or some thing. 
I would be smart to make LC's of them before they are gone huh ???
if yes then ....
been looking round the Teks. but any one got a WOOT WOOT good one.
Edited by smily (10/07/11 08:32 PM)
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 13 years, 1 month
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Re: LC Question [Re: smily]
#15193040 - 10/07/11 06:49 PM (13 years, 3 months ago) |
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: LC Question [Re: 760Rollz]
#15193054 - 10/07/11 06:53 PM (13 years, 3 months ago) |
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My favorite LC recipe as far as sugar based LC's go is Light Malt Extract LC. Add .6 of a gram of LME to 400ML of water in a quart jar. For your jar lid silicone an injection port and whatman syringe filter into place as shown below. This allows you to be able to shake your jars which will distribute the mycelium without getting your filter wet or inviting in contaminates. PC for 40 minutes at 15 PSI and your good to go.

Here is an LME LC at 10 days from inoculation with a few kernels of colonized grain spawn. This was done in front of a laminar flow hood. Culture you are seeing in the picture is Shiitake.
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smily
lookin 4 my ass wit both handz



Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 12 days, 10 hours
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Evil whats up with the lids are they bought or made ???
and thanks ROLLS
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: LC Question [Re: smily]
#15193086 - 10/07/11 07:03 PM (13 years, 3 months ago) |
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Home made but you can go about getting yourself something similar in a few different ways.
TheMadSeason sells premade lids I do believe, or you can purchase the syringe filters and inoculation ports from them and make your own. I highly recommend this style of lid for LC's.
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 13 years, 1 month
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Those are pretty sick jars. Do you make the lids by simpley drilling small holes in them and then fitting it with the fittings?
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: LC Question [Re: 760Rollz]
#15193110 - 10/07/11 07:09 PM (13 years, 3 months ago) |
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RTV Silicone Whatman syringe filter Inoculation port Drill Some time
I drill my holes in the plastic lid as needed. Different sized inoculation ports will call for different sized holes. I then apply a thin layer of RTV to the backside of the inoculation port and fit it through the hole I have made in the jar lid. I then add more RTV around the sides of the port on both the top and bottom. I do the same for the syringe filter.
Here are some pictures of the process:

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smily
lookin 4 my ass wit both handz



Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 12 days, 10 hours
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Re: LC Question [Re: smily]
#15193114 - 10/07/11 07:10 PM (13 years, 3 months ago) |
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did you make a lid TEK by chance ????
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: LC Question [Re: smily]
#15193123 - 10/07/11 07:13 PM (13 years, 3 months ago) |
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I did not, its pretty straight forward tho, as long as you have a nice tight seal around your components and your metal/plastic jar lid you will be fine.
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 13 years, 1 month
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Re: LC Question [Re: smily]
#15193144 - 10/07/11 07:20 PM (13 years, 3 months ago) |
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Cool I totally dig it. Why the two holes though? Is one specifically to inject with and the other to withdraw the LC once mycelium grows. Please forgive me if this is a stupid question. I want to strat working with LCs and this seems like it is a great method to do so. I just want to fully understand whats going on here.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: LC Question [Re: 760Rollz]
#15193153 - 10/07/11 07:22 PM (13 years, 3 months ago) |
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The one hole is used to install your injection port for injecting your spore solution or withdrawing LC when your mixture is done colonizing. The other holes is your gas exchange.
A whatman syringe filter allows filtered air to enter the jar while PCing so that a vacume is not created while pressure cooking. As well the whatman allows for gas exchange during colonization, and equalized pressure as you withdraw your solution.
The Whatman syringe filters are rated at .2 micron.
Edited by EvilMushroom666 (10/07/11 07:33 PM)
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760Rollz
Out the game for now. Peace all



Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 13 years, 1 month
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Gotcha Thanks.
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Edited by 760Rollz (10/09/11 03:52 PM)
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Eburger
StrangerThenMost



Registered: 06/16/11
Posts: 114
Loc: NoWhereFast
Last seen: 4 years, 13 days
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What type of bits do you use to drill your holes? I always end up cracking some of the plastic lids with the 1/2 bit. Do you drill from the top down or the bottom side?
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smily
lookin 4 my ass wit both handz



Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 12 days, 10 hours
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Re: LC Question [Re: Eburger]
#15193408 - 10/07/11 08:29 PM (13 years, 3 months ago) |
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U crack it's because when it builds up from the digging process the chips cool and then the twist of the bit snaps the material.
on a saw blade you use the negative rake angle and this prevents that don't know what does translate andel bits
preheat your plastic a little bit to get it warm and drill it very slowly
I'm a cabinet maker and a carpenter so I can get through pretty much any material
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Slaithenn
Stranger

Registered: 05/19/10
Posts: 122
Last seen: 10 years, 8 months
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Re: LC Question [Re: Eburger]
#15193419 - 10/07/11 08:31 PM (13 years, 3 months ago) |
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Use sharp bits, be gentle, and don't be afraid to go slow... Put them up side down on a board or something you don't mind drilling into. 
Edit: I have no problem with spade bits for smaller then an inch holes, but for big holes fostner or something like that bits are amazing.
Also, the injection port's are a bit over rated IMHO (of corse). A small hole and some silicone works just fine fore me.
Edited by Slaithenn (10/07/11 08:34 PM)
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smily
lookin 4 my ass wit both handz



Registered: 07/13/06
Posts: 2,592
Loc: Lee HO FooKs
Last seen: 12 days, 10 hours
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Quote:
Slaithenn said:
Also, the injection port's are a bit over rated IMHO (of corse). A small hole and some silicone works just fine fore me.
Sure, but this is like the GO PRO option for longevity.
To each their own........
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Slaithenn
Stranger

Registered: 05/19/10
Posts: 122
Last seen: 10 years, 8 months
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Re: LC Question [Re: smily]
#15193587 - 10/07/11 09:05 PM (13 years, 3 months ago) |
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Got lots of silicone on hand any way, takes two seconds to make, requires less bit changes, it's cheaper, and holds up almost as well. So due to the facts that it takes less manual labor and it's cheaper. I just assume put a small hole with two dabs of silicone. Done right, it looks very clean.
Not that injector ports aren't nice.... Just don't see the point in going out of your way to get them is all. By all means though, if you like them go for it!
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