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Offline760Rollz
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Which tends to colonize faster?
    #15192728 - 10/07/11 05:32 PM (13 years, 3 months ago)

Of the two which tends to show sizes of colonization faster a BRF cake or an agar dish?


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OfflineTheMackologist
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Re: Which tends to colonize faster? [Re: 760Rollz]
    #15192733 - 10/07/11 05:33 PM (13 years, 3 months ago)

I believe an agar dish


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OfflineFuckMeRunnin
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Re: Which tends to colonize faster? [Re: TheMackologist]
    #15192791 - 10/07/11 05:47 PM (13 years, 3 months ago)

Honestly I have always had pretty slow growth of cubes on agar. I would say its about equal really. Depending on what method you use. Overall I would say its pretty even.


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OfflineLord_McLovin
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Re: Which tends to colonize faster? [Re: FuckMeRunnin]
    #15192954 - 10/07/11 06:24 PM (13 years, 3 months ago)

I agree.
Cubes seem to colonize pretty slowly on agar.
Why do you wanna know?


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Offline760Rollz
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Re: Which tends to colonize faster? [Re: Lord_McLovin]
    #15192999 - 10/07/11 06:39 PM (13 years, 3 months ago)

Because I inoculated some jars and dropped some spores on some agar the other night at the same time.  The spores were at least a 3rd generation hand me down and I cant imagine they are the most sterile of prints.  I am just wondering which would be more likely to show any signs of growth first.


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OfflineWing
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Re: Which tends to colonize faster? [Re: 760Rollz]
    #15193005 - 10/07/11 06:41 PM (13 years, 3 months ago)

I always see fast growth on agar no matter what method I use to inoculate (spore, mycelium, pin, etc.)


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OfflineLord_McLovin
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Re: Which tends to colonize faster? [Re: Wing]
    #15193033 - 10/07/11 06:46 PM (13 years, 3 months ago)

What kind of agar do you use and what is your definition of fast?


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Offline760Rollz
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Re: Which tends to colonize faster? [Re: Lord_McLovin]
    #15193063 - 10/07/11 06:56 PM (13 years, 3 months ago)

I use this recipe
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )

And I'm not really looking for fast for say.  I just want to know which one, the jars or the agar might start to show growth first.


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OfflineRogerRabbitM
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Re: Which tends to colonize faster? [Re: 760Rollz]
    #15193505 - 10/07/11 08:49 PM (13 years, 3 months ago)

You'll see growth in the flat, two dimensional plane of a petri dish days before you'll see it in any other substrate.

Anyone who thinks cubes grow slow on agar isn't using a proper agar recipe such as MEA.  They're rocket ship fast.
RR


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Offline760Rollz
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Re: Which tends to colonize faster? [Re: RogerRabbit]
    #15193557 - 10/07/11 08:58 PM (13 years, 3 months ago)

Thank you :biggrin:


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OfflineLord_McLovin
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Re: Which tends to colonize faster? [Re: RogerRabbit]
    #15196321 - 10/08/11 02:34 PM (13 years, 3 months ago)

Quote:

RogerRabbit said:
You'll see growth in the flat, two dimensional plane of a petri dish days before you'll see it in any other substrate.

Anyone who thinks cubes grow slow on agar isn't using a proper agar recipe such as MEA.  They're rocket ship fast.
RR




I use Stamet's PDYA recipe (as stated in TMC). Is it no good?
I don't have any caramellization issues, if you think that's the problem.


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Offline760Rollz
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Re: Which tends to colonize faster? [Re: Lord_McLovin]
    #15197429 - 10/08/11 07:12 PM (13 years, 3 months ago)

Today I was just turned onto an agar recipe that uses dog food. I was taught it by a "Trusted Cultivator" so I would say it's legit.  The recipe calls for 10 grams dry dog food, 10 grams agar, and 500ml water.  I just got done PC-ing 6 dishes of dog food agar.  I'm going to inoculate them with some Lipa Yai spores once they cool down.


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OfflineHeadFood
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Re: Which tends to colonize faster? [Re: RogerRabbit]
    #15198261 - 10/08/11 10:28 PM (13 years, 3 months ago)

Quote:

RogerRabbit said:
They're rocket ship fast.
RR





That's right....:rocket:ROCKET SHIP FAST! :rocket:


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