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Psychedelicious
Psychonaut



Registered: 09/06/09
Posts: 238
Last seen: 12 years, 5 months
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Burning a hole in your injection port?
#15182866 - 10/05/11 06:43 PM (13 years, 3 months ago) |
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I recently inoculated a liquid culture (with whatmans filter for GE and one of those rubber medical inoculation ports) after flame-sterilizing the needle until glowing bright red with a butane torch. I squirted out a small amount of solution to "cool the needle" which basicaly evaporated violently on exiting the needle. After puncturing the rubber injection port, it seemed as if it left a visible hole in the port. Now, I know the port is thick, and maybe the hole was just on the outer surface of the port, but I'm afraid that it is a contamination vector.
Just to be safe, I put a tiny dab of silicon over it (not a glob, just a tiny layer enough to seal any hole) and set it aside.
Thoughts?
-------------------- <metatron> i dont think the surface area of a mushroom is quite ideal to administer psychoactive compounds anally
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Buthoscorpio
Registered: 01/05/11
Posts: 96
Last seen: 3 years, 4 months
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Quote:
Thoughts?
Wait and see what will happen.
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Guesstimate
Picklocker Of Consciousness


Registered: 09/16/11
Posts: 485
Last seen: 12 years, 3 months
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Re: Burning a hole in your injection port? [Re: Buthoscorpio]
#15185066 - 10/06/11 06:02 AM (13 years, 3 months ago) |
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dosent seem like anything dangerous just let it be not much u can do really.
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prismism



Registered: 05/11/09
Posts: 5,570
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Skip flame sterilizing. Unless you inoculate in open air or you're just plain inexperienced, you should be able to get away without doing it.
-------------------- ephemeral anomalous
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Burning a hole in your injection port? [Re: prismism]
#15186143 - 10/06/11 11:55 AM (13 years, 3 months ago) |
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You should also stop wearing condoms when you are intimate with women. I mean if you pull out you should be fine and more then likely will not get her pregnant.
In regards to the question:
As long as you have cooled the needle before puncturing the injection port you will be fine.
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prismism



Registered: 05/11/09
Posts: 5,570
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Quote:
EvilMushroom666 said: You should also stop wearing condoms when you are intimate with women. I mean if you pull out you should be fine and more then likely will not get her pregnant.
Your parallel blows. Condoms are for pussies. (see what I did there?)
-------------------- ephemeral anomalous
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Burning a hole in your injection port? [Re: prismism]
#15186604 - 10/06/11 01:34 PM (13 years, 3 months ago) |
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I cool the needle w/ an alcohol soaked paper towel before inoculations. Flame the needle between each jar. Personally, I hate those injection ports. You have to fill them w/ RTV anyways, so why not just skip them & use RTV. It's never failed me.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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SexySmurff
HyperQube



Registered: 06/21/04
Posts: 522
Loc: HyperSpace
Last seen: 4 years, 9 months
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lol, i don't get why it's so hard to wait for the needle to cool down.
-------------------- Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Burning a hole in your injection port? [Re: SexySmurff]
#15186666 - 10/06/11 01:44 PM (13 years, 3 months ago) |
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Me either 
Especially if you're working in a SAB. The time it takes for you to move the needle into the SAB is enough.
-------------------- AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING     
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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