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OfflinePsychedelicious
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Registered: 09/06/09
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Burning a hole in your injection port?
    #15182866 - 10/05/11 06:43 PM (13 years, 3 months ago)

I recently inoculated a liquid culture (with whatmans filter for GE and one of those rubber medical inoculation ports) after flame-sterilizing the needle until glowing bright red with a butane torch. I squirted out a small amount of solution to "cool the needle" which basicaly evaporated violently on exiting the needle. After puncturing the rubber injection port, it seemed as if it left a visible hole in the port. Now, I know the port is thick, and maybe the hole was just on the outer surface of the port, but I'm afraid that it is a contamination vector.

Just to be safe, I put a tiny dab of silicon over it (not a glob, just a tiny layer enough to seal any hole) and set it aside.

Thoughts?


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<metatron> i dont think the surface area of a mushroom is quite ideal to administer psychoactive compounds anally

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OfflineButhoscorpio
Registered: 01/05/11
Posts: 96
Last seen: 3 years, 4 months
Re: Burning a hole in your injection port? [Re: Psychedelicious]
    #15184794 - 10/06/11 02:39 AM (13 years, 3 months ago)

Quote:

Thoughts?




Wait and see what will happen.

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OfflineGuesstimate
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Registered: 09/16/11
Posts: 485
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Re: Burning a hole in your injection port? [Re: Buthoscorpio]
    #15185066 - 10/06/11 06:02 AM (13 years, 3 months ago)

dosent seem like anything dangerous just let it be not much u can do really.

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Invisibleprismism
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Registered: 05/11/09
Posts: 5,570
Re: Burning a hole in your injection port? [Re: Psychedelicious]
    #15186027 - 10/06/11 11:25 AM (13 years, 3 months ago)

Skip flame sterilizing. Unless you inoculate in open air or you're just plain inexperienced, you should be able to get away without doing it.


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ephemeral anomalous

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InvisibleEvilMushroom666
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Re: Burning a hole in your injection port? [Re: prismism]
    #15186143 - 10/06/11 11:55 AM (13 years, 3 months ago)

You should also stop wearing condoms when you are intimate with women. I
mean if you pull out you should be fine and more then likely will not get
her pregnant.

In regards to the question:

As long as you have cooled the needle before puncturing the injection port
you will be fine.

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Invisibleprismism
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Registered: 05/11/09
Posts: 5,570
Re: Burning a hole in your injection port? [Re: EvilMushroom666]
    #15186231 - 10/06/11 12:15 PM (13 years, 3 months ago)

Quote:

EvilMushroom666 said:
You should also stop wearing condoms when you are intimate with women. I
mean if you pull out you should be fine and more then likely will not get
her pregnant.




Your parallel blows. Condoms are for pussies. (see what I did there?)


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ephemeral anomalous

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InvisibleTranscendingLife
I Don't Need a Life to Live
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Re: Burning a hole in your injection port? [Re: prismism]
    #15186604 - 10/06/11 01:34 PM (13 years, 3 months ago)

I cool the needle w/ an alcohol soaked paper towel before inoculations.
Flame the needle between each jar.
Personally, I hate those injection ports.
You have to fill them w/ RTV anyways, so why not just skip them & use RTV.
It's never failed me.

:2cents:


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AMU: We Quickly Answer Questions Here
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OfflineSexySmurff
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Re: Burning a hole in your injection port? [Re: TranscendingLife]
    #15186624 - 10/06/11 01:38 PM (13 years, 3 months ago)

lol, i don't get why it's so hard to wait for the needle to cool down.  :shrug:


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Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.

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InvisibleTranscendingLife
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Re: Burning a hole in your injection port? [Re: SexySmurff]
    #15186666 - 10/06/11 01:44 PM (13 years, 3 months ago)

Me either :shrug2:

Especially if you're working in a SAB.
The time it takes for you to move the needle into the SAB is enough.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

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